Tmod4 (NM_016712) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR205184
Tmod4 (Myc-DDK-tagged) - Mouse tropomodulin 4 (Tmod4)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tmod4
Synonyms MTMOD; Sk-Tmod
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR205184 representing NM_016712
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCATCCTATCAGAAGGAACTGGAGAAATACAGAGACATCGACGAAGATGAGATCCTAAGGACCCTGA
GCCCTGAAGAGCTAGAACAGCTGGACTGTGAGCTACAAGAAATGGATCCTGAGAACATGCTCTTGCCAGC
TGGCCTGAGACAACGTGACCAGACAAAGAAGAGTCCAACAGGGCCACTGGACAGAGATGCCCTATTGCAG
TACCTGGAGCAACAGGCGCTAGAAGTCAAAGAGCGTGATGACTTGGTGCCCTACACTGGCGAGAAGAAGG
GAAAACCCTTTATTCAGCCTAAGAGAGAAATCCCAGCACAGGAACAGATTACGCTGGAGCCTGAGCTGGA
GGAAGCGCTGTCCCATGCTACAGATGCTGAAATGTGTGACATTGCAGCCATCCTGGGCATGTATACACTG
ATGAGCAACAAGCAATATTATGATGCCATCTGCAGTGGAGAGATCTGCAACACAGAAGGCATTAGCAGTG
TGGTACAGCCTGACAAGTATAAGCCAGTACCAGATGAACCTCCAAATCCCACAAACATTGAGGAGATGCT
AAAGAGGGTACGGAGTAATGACAAAGAGCTGGAGGAGGTGAACCTCAATAATATCCAGGACATCCCAATA
CCTGTGCTAAGTGACTTGTGTGAAGCAATGAAAACCAATACCTATGTCCGGAGCTTCAGTCTGGTGGCCA
CAAAGAGTGGTGACCCAATCGCCAATGCGGTGGCTGACATGTTGCGTGAGAACCGCAGTCTCCAGAGCCT
GAACATTGAGTCTAACTTCATCAGCAGCACAGGGCTCATGGCTGTGCTAAAGGCAGTTCGGGAAAATGCT
ACTCTCACTGAGCTCCGTGTAGATAACCAGCGCCAGTGGCCTGGTGATGCGGTAGAGATGGAGATGGCCA
CTGTGCTAGAGCAGTGTCCGTCTATTGTCCGCTTCGGGTACCACTTTACACAACAAGGACCACGAGCTCG
GGCAGCCCACGCCATGACCCGGAACAATGAGCTGCGTCGTCAGCAAAAGAAGAGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR205184 representing NM_016712
Red=Cloning site Green=Tags(s)

MSSYQKELEKYRDIDEDEILRTLSPEELEQLDCELQEMDPENMLLPAGLRQRDQTKKSPTGPLDRDALLQ
YLEQQALEVKERDDLVPYTGEKKGKPFIQPKREIPAQEQITLEPELEEALSHATDAEMCDIAAILGMYTL
MSNKQYYDAICSGEICNTEGISSVVQPDKYKPVPDEPPNPTNIEEMLKRVRSNDKELEEVNLNNIQDIPI
PVLSDLCEAMKTNTYVRSFSLVATKSGDPIANAVADMLRENRSLQSLNIESNFISSTGLMAVLKAVRENA
TLTELRVDNQRQWPGDAVEMEMATVLEQCPSIVRFGYHFTQQGPRARAAHAMTRNNELRRQQKKR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_016712
ORF Size 1035 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016712.4
RefSeq Size 1241 bp
RefSeq ORF 1038 bp
Locus ID 50874
UniProt ID Q9JLH8
Cytogenetics 3 40.74 cM
MW 39.3 kDa
Summary Blocks the elongation and depolymerization of the actin filaments at the pointed end. The Tmod/TM complex contributes to the formation of the short actin protofilament, which in turn defines the geometry of the membrane skeleton (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_016712" in other vectors (4)
SKU Description Size Price
MC209871 Tmod4 (untagged) - Mouse tropomodulin 4 (Tmod4), (10ug) 10 ug
$503.00
MG205184 Tmod4 (tGFP-tagged) - Mouse tropomodulin 4 (Tmod4) 10 ug
$657.00
MR205184L3 Lenti ORF clone of Tmod4 (Myc-DDK-tagged) - Mouse tropomodulin 4 (Tmod4) 10 ug
$757.00
MR205184L4 Lenti ORF clone of Tmod4 (mGFP-tagged) - Mouse tropomodulin 4 (Tmod4) 10 ug
$757.00

Plasmid Map for MR205184

Download sequence for

Cloning scheme for MR205184

Circular map for MR205184

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