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Nagk (NM_001164187) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR205154
Nagk (Myc-DDK-tagged) - Mouse N-acetylglucosamine kinase (Nagk), transcript variant 2
Interest in protein/lysate? Submit request here!
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Nagk
Synonyms Gnk
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR205154 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGCGCTTTATGGTGGCGTGGAAGGGGGAGGCACACGGTCCAAAGTCCTTTTACTTTCTGAGGATG
GGCAGATCCTGGCAGAAGCAGATGGACTGAGCACAAACCACTGGCTGATTGGCACAGACCAGTGTGTGGA
GAGGATCAATGAGATGGTGGACAGGGCCAAACAGAAGGCTGGAGTGGATCCTCTGGTGCCCCTTCGAAGC
CTGGGCCTGTCCCTGAGTGGTGGGGAGCAGGAGGATGCAGTGAGGCTCCTGATTGAGGAGTTGAGGCACC
GCTTTCCCAACCTGAGTGAAAACTACTTAATCACCACGGATGCAGCAGGTTCCATCGCCACAGCCACACC
GGACGGTGGGATTGTGCTCATCTCTGGAACAGGCTCCAACTGTAGGCTTATCAACCCTGATGGCTCCGAG
AGTGGCTGTGGAGGCTGGGGCCACATGATGGGAGACGAGGGCTCAGCCTACTGGATTGCACACCAAGCTG
TGAAAATTGTGTTTGATTCCATCGACAACCTGGAGGCAGCTCCTCATGATATTGGCCATGTCAAGCAGGC
CATGTTCGACTATTTCCAGGTGCCAGATCGGCTTGGAATCCTCACCCATTTGTATAGGGACTTTGATAAA
TGCAAGTTTGCTGGATTTTGCCAGAAAATTGCAGAAGGTGCACATCAGGGAGACCCTCTTTCCAGGTACA
TCTTCAGGAAGGCTGGAGAGATGCTGGGCAGACATGTTGTGGCAGTATTGCCAGAGATTGACCCGGTTTT
GTTCCAAGGGGAGCTTGGCCTCCCCATTCTGTGTGTGGGCTCAGTGTGGAAGAGCTGGGAGCTCCTGAAG
GAAGGCTTTCTCCTGGCGCTGACGCTGGGCCGAGAGCAGCAGGCACAGAACTCCTTCTCCAGCTTCACCC
TGATGAAGTTGCGGCATTCTTCTGCATTGGGGGGTGCCAGCCTGGGGGCCAGGCACATTGGATACCACCT
TCCCATGGACTACAGTATCAATGCCATTGCCTTCTATTCCTATACCTTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR205154 protein sequence
Red=Cloning site Green=Tags(s)

MAALYGGVEGGGTRSKVLLLSEDGQILAEADGLSTNHWLIGTDQCVERINEMVDRAKQKAGVDPLVPLRS
LGLSLSGGEQEDAVRLLIEELRHRFPNLSENYLITTDAAGSIATATPDGGIVLISGTGSNCRLINPDGSE
SGCGGWGHMMGDEGSAYWIAHQAVKIVFDSIDNLEAAPHDIGHVKQAMFDYFQVPDRLGILTHLYRDFDK
CKFAGFCQKIAEGAHQGDPLSRYIFRKAGEMLGRHVVAVLPEIDPVLFQGELGLPILCVGSVWKSWELLK
EGFLLALTLGREQQAQNSFSSFTLMKLRHSSALGGASLGARHIGYHLPMDYSINAIAFYSYTF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001164187
ORF Size 1029 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq Size 1321 bp
RefSeq ORF 879 bp
Locus ID 56174
Cytogenetics 6 C3
MW 37.3 kDa
Summary Converts endogenous N-acetylglucosamine (GlcNAc), a major component of complex carbohydrates, from lysosomal degradation or nutritional sources into GlcNAc 6-phosphate. Involved in the N-glycolylneuraminic acid (Neu5Gc) degradation pathway. Also has ManNAc kinase activity.UniProtKB/Swiss-Prot Function
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"NM_001164187" in other vectors (2)
SKU Description Size Price
MR205154L3 Lenti ORF clone of Nagk (Myc-DDK-tagged) - Mouse N-acetylglucosamine kinase (Nagk), transcript variant 2 10 ug
$757.00
MR205154L4 Lenti ORF clone of Nagk (mGFP-tagged) - Mouse N-acetylglucosamine kinase (Nagk), transcript variant 2 10 ug
$757.00

Plasmid Map for MR205154

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR205154
Circular map for MR205154

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