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Kif12 (BC022225) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR205147
Kif12 (Myc-DDK-tagged) - Mouse kinesin family member 12 (cDNA clone MGC:19162 IMAGE:4222522)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Kif12
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR205147 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGAACGTGGGTCTCCCGATGGAGACCCAGCACGGAACCTGGAGCAAGGGCCAGAAGGATCAGAAA
CTCCCATTCAAGTGGTGCTCAGGGTGCGTCCCATGAGCACAGTGGAGCTGCGTCGAGGGGAGCAGAGTGC
ATTGCACTGTTCGGGGACCAGGACTCTGCAGGTGAGCCCGCCGGGTGGAGGCCCCGATGTGGCGTTCCGC
TTTGGCGCCGTACTGGACGGGGCGCGCACGCAGGAGGACGTGTTCAGAGCTTGCGGTGTGAAGCGCCTGG
GTGAGCTGGCGCTGCGCGGTTTCTCCTGCACGGTCTTCACGTTTGGCCAGACTGGTTCTGGGAAGACCTA
CACTCTGACTGGACCTCCTCCGCAGGGGGAAGGGGTGCCCGTCCCCCCAAGCCTGGCTGGCATCATGCAG
AGGACCTTCACCTGGCTATTAGATCGCGTGCAGCACCTGGATTCCCCGGTCACCCTCCGAGCTTCCTACC
TGGAGATCTACAATGAGCAGGTTTGGGACTTGCTGAGTCTGGGGTCTCCACGGCCTCTGCCTGTTAGATG
GACCAAGGCACGAGGCTTCTATGTGGAACAGCTTCGGGTGGTGGAGTTTGGCAGTCTGGAGGCTTTGATG
GAGCTCCTGCAGATGGGTCTCAGCCGTCGTCGGAGCTCCTCACATACCTTGAACCAGGCCTCCAGCAGAA
GCCATGCCCTGCTTACACTCCACATCAGCCGCCCAACTGTGAGTGTCCAGCCTGTGGAAGGGCTGGCTCA
GGGATACCCATTGAGACTCTCTGCCTTTGCATGGATAAAACAACTTGGCTCTGTTGGGTGTATGGGGAGG
AAACCTTTGACCTTGAGACACTGGGACTTTAATACAGACAGCCCTCCCCAAGTTCCCATCAAACCCTGGT
TCACCAGTCTCAGCAGGTACCTCCTGTGGACCTTGGGGAGCCCCCTGTTGGAGGGAAGCTGTGCTTTGTG
GATCTTGCAGGCAGTGAGAAAGTGGCAGCCACAGGATCCCAAGGGCAGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR205147 protein sequence
Red=Cloning site Green=Tags(s)

MEERGSPDGDPARNLEQGPEGSETPIQVVLRVRPMSTVELRRGEQSALHCSGTRTLQVSPPGGGPDVAFR
FGAVLDGARTQEDVFRACGVKRLGELALRGFSCTVFTFGQTGSGKTYTLTGPPPQGEGVPVPPSLAGIMQ
RTFTWLLDRVQHLDSPVTLRASYLEIYNEQVWDLLSLGSPRPLPVRWTKARGFYVEQLRVVEFGSLEALM
ELLQMGLSRRRSSSHTLNQASSRSHALLTLHISRPTVSVQPVEGLAQGYPLRLSAFAWIKQLGSVGCMGR
KPLTLRHWDFNTDSPPQVPIKPWFTSLSRYLLWTLGSPLLEGSCALWILQAVRKWQPQDPKGS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC022225
ORF Size 1029 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC022225, AAH22225
RefSeq Size 3402 bp
RefSeq ORF 1031 bp
Locus ID 16552
Cytogenetics 4 B3
MW 37.9 kDa
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"BC022225" in other vectors (4)
SKU Description Size Price
MC217714 Kif12 (untagged) - Mouse kinesin family member 12 (cDNA clone MGC:19162 IMAGE:4222522), (10ug) 10 ug
$503.00
MG205147 Kif12 (tGFP-tagged) - Mouse kinesin family member 12 (cDNA clone MGC:19162 IMAGE:4222522) 10 ug
$657.00
MR205147L3 Lenti ORF clone of Kif12 (Myc-DDK-tagged) - Mouse kinesin family member 12 (cDNA clone MGC:19162 IMAGE:4222522) 10 ug
$757.00
MR205147L4 Lenti ORF clone of Kif12 (mGFP-tagged) - Mouse kinesin family member 12 (cDNA clone MGC:19162 IMAGE:4222522) 10 ug
$757.00

Plasmid Map for MR205147

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR205147
Circular map for MR205147

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