Ncf4 (NM_008677) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR205075
Ncf4 (Myc-DDK-tagged) - Mouse neutrophil cytosolic factor 4 (Ncf4)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ncf4
Synonyms AI451400; p40phox
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR205075 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCTGGCCCAGCAGCTGCGATCAGAGAGCGACTTTGAGCAGCTTCCAGACGATGTGGCCGTCTCAG
CCAACATCGCTGACATCGAGGAGAAGAGAGGCTTCACCAGCCACTTTGTTTTTGTCATCGAGGTCAAAAC
AAAAGGAGGGTCCAAGTATCTCATCTACCGCCGCTATCGCCAGTTCTACGCCCTGCAGAGCAAGCTGGAG
GAGCGGTTTGGGCCAGAGAGCAAGAACAGCCCTTTCACCTGCAACCTGCCCACATTGCCAGCCAAAGTCT
ACATGGGCGCAAAACAAGAGATCGCTGAGACTCGGATCCCGGCCCTCAATGCCTACATGAAGAACCTCCT
GAGCCTGCCGGTCTGCGTGCTGATGGACCCCGACGTCAGGATCTTCTTCTATCAGTCTGCATACGATGCT
GAGCAGGTGCCCCAGGCACTCCGCAGGCTCCGACCGCGCACGCGCAAAATCAAGGGTGTGTCTCCACAAG
GGGCCATCATGGATCGCATGGAAGCGCCAAGAGCAGAGGCCTTGTTTGACTTCACTGGGAACAGCAAATT
GGAGCTAAGTTTCAAAGCTGGAGATGTGATCTTCCTTCTCAGTAAGATCAACAAAGACTGGCTGGAGGGC
ACCTCCCAGGGAGCCACAGGCATCTTCCCAGGGTCCTTCGTGAAGATCCTTAAGGACTTTCCCGAGGACG
AGGACACCACCAACTGGCTACGATGCTACTTCTATGAAGACACAGGCAAAACCATCAAGGACATTGCGGT
GGAGGAGGACCTCAGCAGCACGCCCCTGTTCAAAGACCTGCTAGCGCTCATGAGGCGTGAGTTCCAGAGA
GAAGACATCGCCCTTAGCTACCAGGATGCTGAGGGGGACTTGGTTAGGCTGCTGTCAGATGAGGATGTGG
GACTCATGGTGAAGCAAGCCCGAGGCCTCCCTTCCCAGAAGCGCCTCTTCCCCTGGAAGCTGCATGTCAC
ACAGAAGGACAACTACAGTGTCTACAACACTGTCCCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR205075 protein sequence
Red=Cloning site Green=Tags(s)

MALAQQLRSESDFEQLPDDVAVSANIADIEEKRGFTSHFVFVIEVKTKGGSKYLIYRRYRQFYALQSKLE
ERFGPESKNSPFTCNLPTLPAKVYMGAKQEIAETRIPALNAYMKNLLSLPVCVLMDPDVRIFFYQSAYDA
EQVPQALRRLRPRTRKIKGVSPQGAIMDRMEAPRAEALFDFTGNSKLELSFKAGDVIFLLSKINKDWLEG
TSQGATGIFPGSFVKILKDFPEDEDTTNWLRCYFYEDTGKTIKDIAVEEDLSSTPLFKDLLALMRREFQR
EDIALSYQDAEGDLVRLLSDEDVGLMVKQARGLPSQKRLFPWKLHVTQKDNYSVYNTVP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008677
ORF Size 1017 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008677.1, NP_032703.1
RefSeq Size 1463 bp
RefSeq ORF 1020 bp
Locus ID 17972
UniProt ID P97369
Cytogenetics 15 37.1 cM
MW 38.7 kDa
Summary Component of the NADPH-oxidase, a multicomponent enzyme system responsible for the oxidative burst in which electrons are transported from NADPH to molecular oxygen, generating reactive oxidant intermediates. It may be important for the assembly and/or activation of the NADPH-oxidase complex.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_008677" in other vectors (3)
SKU Description Size Price
MG205075 Ncf4 (tGFP-tagged) - Mouse neutrophil cytosolic factor 4 (Ncf4) 10 ug
$657.00
MR205075L3 Lenti ORF clone of Ncf4 (Myc-DDK-tagged) - Mouse neutrophil cytosolic factor 4 (Ncf4) 10 ug
$757.00
MR205075L4 Lenti ORF clone of Ncf4 (mGFP-tagged) - Mouse neutrophil cytosolic factor 4 (Ncf4) 10 ug
$757.00

Plasmid Map for MR205075

Download sequence for

Cloning scheme for MR205075

Circular map for MR205075

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