Rad51ap1 (NM_009013) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR205010
Rad51ap1 (Myc-DDK-tagged) - Mouse RAD51 associated protein 1 (Rad51ap1)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rad51ap1
Synonyms 2510006L10Rik; RAB22
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR205010 representing NM_009013
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGCGTCCTATCAGAAATAGAAAACCAATAAATTATTCACAGTTTGAAGATTCTGGCAATGACTCTG
ATGATGATTTTATTTCTTCATCCACACCTGTAAACAAGTCAAAAACTGTGCCAAAGGTATTAAAGCAAGA
CAAACCAAAACCTAACTTGAAGAATCTCCAAAAAGAAGAGGTCTTACCAACAGAACCCCCTAAAAAAAGG
GTGGCTTTAGATGACAAGGTCTTCCAAAGAGGTCTAGAAGTTGCCCTGGCTTTATCTGTGAAGGAACTTC
CAACACTCACCAACCAAGTAAAGAAGTCCAAAGAGAAAAGCACTGACAAACAAGGCAAAGAGAAAACAGA
AAATACGGGTAAACCTCCTCATGTCTCTAACTGCAGTGTAGCCAGTGATGATGTAGAAGATTTGGACAAG
ATCACAGAGGAGGGGGACGCCTCGAGTGTTGAAGGGGAGAGGAAGTCACCATCTCAAGCCAAGGCTCCGC
GGAGGAGAGCACCTTCTGAAGGCAGCGATGGCAGCAGTGCTAACGACACGGAGTCGGAGAGCGCCACTGG
TGAAGGTTCAGAAAGTGATCCTGATTTTGATGAGAGTAAAGAAAGTGATGAAGACTTTGGTGTGAGAAGA
AGTAAAGAATCAAAGAAGAAAACAGTGCAGAAGAAACCTGCAGGGGAAAAGAAGGAAAGGAAATCAAAAC
CCAAATGTGAGGCATCAGTGACTTCAGTAGACCCAGCTCCAGCTGCCATCAAGTCAGGATCTCCATCTTT
ACCCCAGGCAGTTGGTCTTCCTTCTGAGGCCACTAGGAAACCAGCAATAATGTGCAGCCCTTCAGCTGAA
AGCAAGAGGCCCAAGTGGGTCCCCCCAGCTGCCTCTGGGAGCAGGAACTCTAGCAGCAATGCATTGGCAG
GAACACCTGCCAAGTCCCCGAGTCAGAGCCTGCGTCTGGGCCTCTCCCGATTAGCGCCAGTTAAGCGCTT
GCATCCAAGTGCCACAAGCAGCCAAGTGCGG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR205010 representing NM_009013
Red=Cloning site Green=Tags(s)

MVRPIRNRKPINYSQFEDSGNDSDDDFISSSTPVNKSKTVPKVLKQDKPKPNLKNLQKEEVLPTEPPKKR
VALDDKVFQRGLEVALALSVKELPTLTNQVKKSKEKSTDKQGKEKTENTGKPPHVSNCSVASDDVEDLDK
ITEEGDASSVEGERKSPSQAKAPRRRAPSEGSDGSSANDTESESATGEGSESDPDFDESKESDEDFGVRR
SKESKKKTVQKKPAGEKKERKSKPKCEASVTSVDPAPAAIKSGSPSLPQAVGLPSEATRKPAIMCSPSAE
SKRPKWVPPAASGSRNSSSNALAGTPAKSPSQSLRLGLSRLAPVKRLHPSATSSQVR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009013
ORF Size 1011 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009013.4
RefSeq Size 1835 bp
RefSeq ORF 1014 bp
Locus ID 19362
UniProt ID Q8C551
Cytogenetics 6 61.92 cM
MW 36.7 kDa
Summary May participate in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Functionally cooperates with PALB2 in promoting of D-loop formation by RAD51. Binds to single and double stranded DNA, and is capable of aggregating DNA. Also binds RNA (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_009013" in other vectors (4)
SKU Description Size Price
MC209282 Rad51ap1 (untagged) - Mouse RAD51 associated protein 1 (Rad51ap1), (10ug) 10 ug
$503.00
MG205010 Rad51ap1 (tGFP-tagged) - Mouse RAD51 associated protein 1 (Rad51ap1) 10 ug
$489.00 $657.00
MR205010L3 Lenti ORF clone of Rad51ap1 (Myc-DDK-tagged) - Mouse RAD51 associated protein 1 (Rad51ap1) 10 ug
$757.00
MR205010L4 Lenti ORF clone of Rad51ap1 (mGFP-tagged) - Mouse RAD51 associated protein 1 (Rad51ap1) 10 ug
$757.00

Plasmid Map for MR205010

Download sequence for

Cloning scheme for MR205010

Circular map for MR205010

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