Mat2b (NM_134017) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR204961
Mat2b (Myc-DDK-tagged) - Mouse methionine adenosyltransferase II, beta (Mat2b), transcript variant 1
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mat2b
Synonyms 1110064C04Rik; 2410018D16Rik; AI182287; AU022853; MAT-II; MATIIbeta; TGR
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR204961 representing NM_134017
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGGGCCGGGAGAAGGAGCTCTCCATCCACTTCGTCCCCGGCTGCTGCCAGCTGGTGGAGGAGGAAG
TGAACATCCCTAGCCGGCGGGTTCTCATTACTGGTGCCACTGGGCTTCTTGGCAGAGCAGTTTACAAAGA
GTTTCAGCAGAGCAACTGGCACACCGTTGGCTGTGGCTTTCGAAGAGCAAGACCAAAATTCGAACAAGTG
AACCTGTTGGATTCTGAAGCTGTTCATCACCTCATTCATGATTTCCAGCCTCATGTCATAGTGCATTGTG
CTGCAGAGAGAAGACCTGATGTTGTTGAGAGTCAGCCAGATGCTGCTTCCCAGCTGAATGTGGGTGCCTC
TGGGAACTTGGCAAAGGAGGCAGCTGCGATTGGAGCATTTCTCATCTACATTAGCTCAGATTATGTGTTT
GATGGCACAAATCCCCCTTACACAGAAGAAGATATACCAAGTCCCCTGAATCTATATGGAAAAACAAAAT
TAGATGGAGAAAAAGCAGTCCTGGAGAATAATTTAGGGGCTGCTGTGTTGAGAATTCCTGTTCTGTATGG
GGAAGTTGAAAAGCTTGAAGAAAGTGCTGTGACTGTTATGTTCGACAAGGTGCAGTTCAGCAACAAGTCA
GCAAACATGGACCATTGGCAGCAGAGGTTCCCCACACATGTGAAAGACGTAGCCAGTGTGTGCCGGCAGC
TGGCAGAGAAGAGGATGCTGGATCCATCCATTAAGGGAACCTTTCACTGGTCTGGCAATGAGCAGATGAC
CAAGTATGAAATGGCGTGTGCAATTGCAGATGCCTTCAACCTCCCCAGCAGCCACTTAAGACCTATTACT
GACAGTCCTGTCATAGGAGCACAGCGCCCCAAAAATGCTCAGCTCGATTGCTCCAAATTGGAGACGTTGG
GCATTGGCCAGAGGACACCATTTCGAACTGGAATCAAAGAATCTCTCTGGCCTTTCCTCATCGACAAGAG
ATGGAGACAGACTGTCTTTCAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR204961 representing NM_134017
Red=Cloning site Green=Tags(s)

MVGREKELSIHFVPGCCQLVEEEVNIPSRRVLITGATGLLGRAVYKEFQQSNWHTVGCGFRRARPKFEQV
NLLDSEAVHHLIHDFQPHVIVHCAAERRPDVVESQPDAASQLNVGASGNLAKEAAAIGAFLIYISSDYVF
DGTNPPYTEEDIPSPLNLYGKTKLDGEKAVLENNLGAAVLRIPVLYGEVEKLEESAVTVMFDKVQFSNKS
ANMDHWQQRFPTHVKDVASVCRQLAEKRMLDPSIKGTFHWSGNEQMTKYEMACAIADAFNLPSSHLRPIT
DSPVIGAQRPKNAQLDCSKLETLGIGQRTPFRTGIKESLWPFLIDKRWRQTVFH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_134017
ORF Size 1002 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_134017.2, NP_598778.1
RefSeq Size 1870 bp
RefSeq ORF 1005 bp
Locus ID 108645
UniProt ID Q99LB6
Cytogenetics 11 A5
MW 37.4 kDa
Summary Regulatory subunit of S-adenosylmethionine synthetase 2, an enzyme that catalyzes the formation of S-adenosylmethionine from methionine and ATP. Regulates MAT2A catalytic activity by changing its kinetic properties, increasing its affinity for L-methionine. Can bind NADP (in vitro).UniProtKB/Swiss-Prot Function
Reviews
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_134017" in other vectors (3)
SKU Description Size Price
MG204961 Mat2b (tGFP-tagged) - Mouse methionine adenosyltransferase II, beta (Mat2b) 10 ug
$657.00
MR204961L3 Lenti ORF clone of Mat2b (Myc-DDK-tagged) - Mouse methionine adenosyltransferase II, beta (Mat2b), transcript variant 1 10 ug
$757.00
MR204961L4 Lenti ORF clone of Mat2b (mGFP-tagged) - Mouse methionine adenosyltransferase II, beta (Mat2b), transcript variant 1 10 ug
$757.00

Plasmid Map for MR204961

Download sequence for

Cloning scheme for MR204961

Circular map for MR204961

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products