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Amd1 (NM_009665) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR204954
Amd1 (Myc-DDK-tagged) - Mouse S-adenosylmethionine decarboxylase 1 (Amd1)
Interest in protein/lysate? Submit request here!
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Amd1
Synonyms AdoMetDC; adoMetDC1; Amd-1; SAMDC; SAMDC 1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR204954 representing NM_009665
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAGCTGCACATTTTTTCGAAGGGACGGAGAAACTGCTGGAGGTCTGGTTTTCCAGACAGCAGTCCG
ACGCAAGCCAGGGATCTGGGGATCTTCGTACCATCCCAAGATCTGAATGGGATGTCCTTTTGAAGGATGT
GCAGTGCTCAATCATAAGTGTGACAAAGACTGACAAGCAGGAAGCTTATGTACTCAGTGAGAGTAGCATG
TTTGTCTCCAAGAGACGTTTCATTTTGAAGACATGTGGTACCACCCTCTTACTGAAAGCACTGGTTCCCC
TGTTGAAGCTTGCTAGGGATTACAGTGGGTTTGACTCAATTCAAAGCTTCTTTTATTCTCGTAAGAATTT
CATGAAGCCTTCTCACCAAGGGTACCCACACCGGAATTTCCAGGAAGAAATCGAGTTTCTTAATGCAATT
TTCCCAAATGGAGCAGCATATTGTATGGGACGCATGAATTCTGACTGCTGGTACTTGTATACTTTGGATT
TCCCAGAGAGCCGAGTAATCAGTCAGCCAGATCAAACACTGGAAATTCTGATGAGTGAGCTTGACCCAGC
AGTTATGGACCAGTTCTACATGAAAGATGGTGTTACTGCAAAGGATGTCACTCGTGAGAGTGGAATTCGT
GACCTGATACCAGGTTCTGTCATTGATGCCACACTGTTCAATCCTTGTGGCTACTCGATGAATGGAATGA
AATCGGATGGAACATATTGGACTATTCACATCACTCCAGAACCAGAATTTTCTTATGTTAGCTTTGAAAC
AAACCTAAGTCAGACCTCCTATGACGACCTGATCAGGAAAGTTGTGGAAGTCTTCAAGCCAGGAAAATTT
GTGACCACCTTGTTTGTTAATCAGAGTTCTAAATGTCGCACAGTGCTTTCTTCGCCCCAGAAGATTGACG
GCTTTAAACGTCTTGATTGCCAGAGTGCTATGTTCAACGATTACAATTTTGTTTTTACCAGTTTTGCTAA
GAAACAGCAACAACAGCAGAGT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR204954 representing NM_009665
Red=Cloning site Green=Tags(s)

MEAAHFFEGTEKLLEVWFSRQQSDASQGSGDLRTIPRSEWDVLLKDVQCSIISVTKTDKQEAYVLSESSM
FVSKRRFILKTCGTTLLLKALVPLLKLARDYSGFDSIQSFFYSRKNFMKPSHQGYPHRNFQEEIEFLNAI
FPNGAAYCMGRMNSDCWYLYTLDFPESRVISQPDQTLEILMSELDPAVMDQFYMKDGVTAKDVTRESGIR
DLIPGSVIDATLFNPCGYSMNGMKSDGTYWTIHITPEPEFSYVSFETNLSQTSYDDLIRKVVEVFKPGKF
VTTLFVNQSSKCRTVLSSPQKIDGFKRLDCQSAMFNDYNFVFTSFAKKQQQQQS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009665
ORF Size 1002 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009665.5, NP_033795.1
RefSeq Size 3206 bp
RefSeq ORF 1005 bp
Locus ID 11702
UniProt ID P0DMN7
Cytogenetics 10 21.85 cM
MW 38.3 kDa
Summary Essential for biosynthesis of the polyamines spermidine and spermine. Promotes maintenance and self-renewal of embryonic stem cells, by maintaining spermine levels.UniProtKB/Swiss-Prot Function
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"NM_009665" in other vectors (3)
SKU Description Size Price
MC202776 Amd1 (untagged) - Mouse S-adenosylmethionine decarboxylase 1 (Amd1), (10ug) 10 ug
$457.00
MR204954L3 Lenti ORF clone of Amd1 (Myc-DDK-tagged) - Mouse S-adenosylmethionine decarboxylase 1 (Amd1) 10 ug
$757.00
MR204954L4 Lenti ORF clone of Amd1 (mGFP-tagged) - Mouse S-adenosylmethionine decarboxylase 1 (Amd1) 10 ug
$757.00

Plasmid Map for MR204954

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR204954
Circular map for MR204954

file-alt Citations

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