Cnmd (NM_010701) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR204953
Lect1 (Myc-DDK-tagged) - Mouse leukocyte cell derived chemotaxin 1 (Lect1)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cnmd
Synonyms Bricd3; ChM-I; Chmd; Lect1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR204953 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACAGAGAACTCAGACAAAGTTCCTATCACCATGGTAGGGCCTGAGGACGTTGAGTTTTGCAGTCCCC
CGGCGTACACCACCGTCACCGTGAAGCCCTCCGGGAGCCCAACACGGCTGCTCAAGGTAGGAGCTGTGGT
CCTCATTTCTGGCGCGGTACTGCTGCTCTTCGGGGCCATCGGGGCCTTCTACTTCTGGAAGGGGAATGAC
AATCACATTTACAATGTTCATTACAGTATGAGTATCAATGGGAAACTACAAGATGGGTCAATGGAAATAG
ATGCTGTGAACAACTTGGAGACCTTTAAAATGGGAAGCGGAGCGGAAGAAGCAATTGAAGTCAACGATTT
TAAAAATGGCATCACTGGGATCCGTTTTGCTGGAGGAGAGAAGTGCTACATCAAAGCACAGGTGAAGGCT
CGCATCCCTGAGGTGGGCACAGTGACCAAGCAGAGCATCTCTGAACTGGAAGGCAAGATCATGCCAGCTA
ACTACGAAGAGAACTCGCTGATTTGGGTGGCCGTGGACCAGCCTGTGAAGGACAGCAGCTTCTTGAGCTC
TAAAATCCTTGAACTCTGTGGCGACCTGCCGATTTTCTGGCTTAAGCCCATGTATCCAAAAGAAATCCAG
AGAGAGAGAAGAGAAGTCGTGAGAAACAGTGCTCCCTCTACCACAAGAAGACCACACAGCGAACCTCGAG
GTAACGCAGGCCCTGGAAGACTGAGTAACGGAACCAGACCCAATGTTCAGGACGACGCAGAACCTTTCAA
CCCTGACAATCCTTACCACCAGCAGGAAGGAGAAAGCATGACATTTGACCCTAGACTGGACCATGAAGGG
ATCTGCTGTATAGAATGCAGGCGGAGCTACACCCACTGCCAGAAGATCTGTGAACCACTGGGAGGCTACT
ATCCATGGCCTTACAATTACCAAGGATGCCGCTCGGCCTGCAGAGTCGTCATGCCATGCAGCTGGTGGGT
GGCCCGCATCTTGGGCATGGTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR204953 protein sequence
Red=Cloning site Green=Tags(s)

MTENSDKVPITMVGPEDVEFCSPPAYTTVTVKPSGSPTRLLKVGAVVLISGAVLLLFGAIGAFYFWKGND
NHIYNVHYSMSINGKLQDGSMEIDAVNNLETFKMGSGAEEAIEVNDFKNGITGIRFAGGEKCYIKAQVKA
RIPEVGTVTKQSISELEGKIMPANYEENSLIWVAVDQPVKDSSFLSSKILELCGDLPIFWLKPMYPKEIQ
RERREVVRNSAPSTTRRPHSEPRGNAGPGRLSNGTRPNVQDDAEPFNPDNPYHQQEGESMTFDPRLDHEG
ICCIECRRSYTHCQKICEPLGGYYPWPYNYQGCRSACRVVMPCSWWVARILGMV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_010701
ORF Size 1002 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_010701.3
RefSeq Size 1436 bp
RefSeq ORF 1005 bp
Locus ID 16840
UniProt ID Q9Z1F6
Cytogenetics 14 D3
MW 37.2 kDa
Summary Bifunctional growth regulator that stimulates the growth of cultured chondrocytes in the presence of basic fibroblast growth factor (FGF) but inhibits the growth of cultured vascular endothelial cells. May contribute to the rapid growth of cartilage and vascular invasion prior to the replacement of cartilage by bone during endochondral bone development (By similarity). Inhibits in vitro tube formation and mobilization of endothelial cells (By similarity). Plays a role as antiangiogenic factor in cardiac valves to suppress neovascularization.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_010701" in other vectors (4)
SKU Description Size Price
MC205453 Lect1 (untagged) - Mouse leukocyte cell derived chemotaxin 1 (Lect1), (10ug) 10 ug
$457.00
MG204953 Lect1 (tGFP-tagged) - Mouse leukocyte cell derived chemotaxin 1 (Lect1) 10 ug
$657.00
MR204953L3 Lenti ORF clone of Lect1 (Myc-DDK-tagged) - Mouse leukocyte cell derived chemotaxin 1 (Lect1) 10 ug
$757.00
MR204953L4 Lenti ORF clone of Lect1 (mGFP-tagged) - Mouse leukocyte cell derived chemotaxin 1 (Lect1) 10 ug
$757.00

Plasmid Map for MR204953

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Cloning scheme for MR204953

Circular map for MR204953

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