B3galnt1 (NM_020026) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR204877
B3galnt1 (Myc-DDK-tagged) - Mouse UDP-GalNAc:betaGlcNAc beta 1,3-galactosaminyltransferase, polypeptide 1 (B3galnt1)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol B3galnt1
Synonyms B3galt3; b3GT3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR204877 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCCGGCTGTCTTGACTGCCCTTCCCAATAGGATGTCACTGAGATCCCTCAAGTGGAGCCTGCTGC
TGCTGTCCCTGCTGAGCTTCCTGGTGATATGGTACCTCAGCCTCCCCCACTACAATGTGATCGAGCGCGT
CAACTGGATGTACTTCTATGAGTATGAGCCAATTTACAGACAAGACTTTCGCTTCACGCTCCGCGAACAC
TCGAACTGTTCTCATCAGAACCCATTCCTGGTCATCCTGGTGACCTCACGCCCCTCAGATGTGAAAGCCA
GACAAGCCATTAGAGTTACTTGGGGTGAGAAAAAGTCCTGGTGGGGATATGAGGTGCTCACGTTTTTCTT
ACTAGGCCAGCAGGCTGAAAGGGAAGACAAAACGTTAGCCCTGTCCTTGGAGGATGAGCACGTTCTCTAT
GGTGATATTATACGGCAAGACTTTCTAGACACATATAATAACTTGACCTTGAAAACCATTATGGCTTTCA
GGTGGGTAATGGAGTTTTGCCCCAATGCCAAGTATATTATGAAAACAGACACTGATGTTTTCATCAACAC
TGGCAATTTAGTCAAGTATCTTTTAAACCTAAACCACTCAGAGAAGTTTTTCACGGGCTATCCTCTAATT
GATAACTATTCCTATAGAGGATTTTTCCATAAAAACCACATTTCATACCAAGAGTACCCCTTCAAGGTGT
TCCCTCCCTACTGCAGCGGGCTGGGCTACATTATGTCCGGCGACCTGGTGCCCAGGGTCTACGAGATGAT
GAGTCACGTGAAGCCCATCAAGTTTGAAGACGTTTATGTTGGCATCTGTTTGAATTTGTTAAAAGTGGAC
ATTCATATTCCAGAAGACACAAACCTTTTCTTTCTGTACAGAATCCACTTGGATGTATGTCAGCTCAGAC
GCGTGATTGCAGCCCATGGCTTTTCTTCCAAGGAGATCATCACATTCTGGCAGGTGATGCTGAGGAACAC
CACATGCCATTAC


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR204877 protein sequence
Red=Cloning site Green=Tags(s)

MAPAVLTALPNRMSLRSLKWSLLLLSLLSFLVIWYLSLPHYNVIERVNWMYFYEYEPIYRQDFRFTLREH
SNCSHQNPFLVILVTSRPSDVKARQAIRVTWGEKKSWWGYEVLTFFLLGQQAEREDKTLALSLEDEHVLY
GDIIRQDFLDTYNNLTLKTIMAFRWVMEFCPNAKYIMKTDTDVFINTGNLVKYLLNLNHSEKFFTGYPLI
DNYSYRGFFHKNHISYQEYPFKVFPPYCSGLGYIMSGDLVPRVYEMMSHVKPIKFEDVYVGICLNLLKVD
IHIPEDTNLFFLYRIHLDVCQLRRVIAAHGFSSKEIITFWQVMLRNTTCHY

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_020026
ORF Size 993 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_020026.1
RefSeq Size 2283 bp
RefSeq ORF 996 bp
Locus ID 26879
UniProt ID Q920V1
Cytogenetics 3 E1
MW 39.4 kDa
Summary Transfers N-acetylgalactosamine onto globotriaosylceramide (PubMed:9417047). Plays a critical role in preimplantation stage embryonic development (PubMed:11463849).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_020026" in other vectors (4)
SKU Description Size Price
MC200320 B3galnt1 (untagged) - Mouse UDP-GalNAc:betaGlcNAc beta 1,3-galactosaminyltransferase, polypeptide 1 (B3galnt1), (10ug) 10 ug
$300.00
MG204877 B3galnt1 (tGFP-tagged) - Mouse UDP-GalNAc:betaGlcNAc beta 1,3-galactosaminyltransferase, polypeptide 1 (B3galnt1) 10 ug
$500.00
MR204877L3 Lenti ORF clone of B3galnt1 (Myc-DDK-tagged) - Mouse UDP-GalNAc:betaGlcNAc beta 1,3-galactosaminyltransferase, polypeptide 1 (B3galnt1) 10 ug
$600.00
MR204877L4 Lenti ORF clone of B3galnt1 (mGFP-tagged) - Mouse UDP-GalNAc:betaGlcNAc beta 1,3-galactosaminyltransferase, polypeptide 1 (B3galnt1) 10 ug
$600.00

Plasmid Map for MR204877

Download sequence for

Cloning scheme for MR204877

Circular map for MR204877

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