Spon2 (NM_133903) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR204867
Spon2 (Myc-DDK-tagged) - Mouse spondin 2, extracellular matrix protein (Spon2)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Spon2
Synonyms 2310045I24Rik; AI504350; M-spondin; Mindin; Mspondin
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR204867 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAAACTTGAGTCTTGCCCTGGGCAGAGCTCTTTGGGTCTTCCTCCTGGCCATGATAGGCTCCACCA
CGAGCCAGCCGCTGGGGGGAGAATCGGTTTGTACAGCCCGGCCTCTGGCTAGATACAGCATCACTTTCAT
TGGCAAGTGGAGCCAGACAGCATTTCCCAAGCAGTACCCCCTGTTCCGGCCCCCTGCACAGTGGTCCTCT
CTGCTGGGGGCAGCTCACAGCTCTGACTACAGCATGTGGCGGAAGAATGAGTATGTCAGCAATGGGCTGA
GGGACTTTGCTGAGCGTGGTGAGGCCTGGGCACTGATGAAGGAGATCGAAGCTGCAGGAGAGAAACTTCA
GAGTGTGCACGCAGTGTTCTCAGCTCCTGCCATCCCTAGCGGTACCGGGCAGACTTCCACAGAGTTAGAG
GTGCACCCTAGGCACTCGCTGGTGTCCTTCGTGGTACGCATTGTCCCCAGCCCTGACTGGTTTGTGGGCA
TCGACAGTCTGGACCTGTGTGAGGGAGGCCGCTGGAAGGAGCAGGTGGTCCTGGATCTTTATCCACACGA
TGCAGGGACCGACAGTGGTTTCACCTTCTCCTCCCCTAACTTTGCAACTATCCCACAAGACACAGTGACT
GAGATAACAGCCTCATCGCCCAGCCACCCAGCAAATTCATTCTACTACCCACGCCTCAAGTCCTTACCTC
CCATCGCCAAAGTGACCTTCGTGCGGCTACAGCATAGTCCCAGGGCCTTTGCCCCACCTTCCCTGGACCT
GGCCAGCCGAGGCAACGAAATCGTTGACAGCCTTTCAGTTCCAGAGACACCGCTGGACTGTGAGGTTTCC
CTGTGGTCATCCTGGGGACTGTGTGGAGGACCATGTGGAAAGTTGGGAGCCAAGAGCAGAACTCGCTATG
TCCGTGTTCAGCCTGCTAACAATGGGACTCCCTGTCCCGAGCTTGAAGAAGAGGCCGAGTGTGCCCCAGA
TAACTGCGTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR204867 protein sequence
Red=Cloning site Green=Tags(s)

MENLSLALGRALWVFLLAMIGSTTSQPLGGESVCTARPLARYSITFIGKWSQTAFPKQYPLFRPPAQWSS
LLGAAHSSDYSMWRKNEYVSNGLRDFAERGEAWALMKEIEAAGEKLQSVHAVFSAPAIPSGTGQTSTELE
VHPRHSLVSFVVRIVPSPDWFVGIDSLDLCEGGRWKEQVVLDLYPHDAGTDSGFTFSSPNFATIPQDTVT
EITASSPSHPANSFYYPRLKSLPPIAKVTFVRLQHSPRAFAPPSLDLASRGNEIVDSLSVPETPLDCEVS
LWSSWGLCGGPCGKLGAKSRTRYVRVQPANNGTPCPELEEEAECAPDNCV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_133903
ORF Size 990 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_133903.1
RefSeq Size 2237 bp
RefSeq ORF 993 bp
Locus ID 100689
UniProt ID Q8BMS2
Cytogenetics 5 B1
MW 36 kDa
Summary Cell adhesion protein that promotes adhesion and outgrowth of hippocampal embryonic neurons. Binds directly to bacteria and their components and functions as an opsonin for macrophage phagocytosis of bacteria. Binds bacterial lipopolysaccharide. Essential in the initiation of the innate immune response and represents a unique pattern-recognition molecule in the ECM for microbial pathogens.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_133903" in other vectors (4)
SKU Description Size Price
MC201295 Spon2 (untagged) - Mouse spondin 2, extracellular matrix protein (Spon2), (10ug) 10 ug
$300.00
MG204867 Spon2 (tGFP-tagged) - Mouse spondin 2, extracellular matrix protein (Spon2) 10 ug
$489.00 $500.00
MR204867L3 Lenti ORF clone of Spon2 (Myc-DDK-tagged) - Mouse spondin 2, extracellular matrix protein (Spon2) 10 ug
$600.00
MR204867L4 Lenti ORF clone of Spon2 (mGFP-tagged) - Mouse spondin 2, extracellular matrix protein (Spon2) 10 ug
$600.00

Plasmid Map for MR204867

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Cloning scheme for MR204867

Circular map for MR204867

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