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BC051665 (NM_199148) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR204864
BC051665 (Myc-DDK-tagged) - Mouse cDNA sequence BC051665 (BC051665)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol BC051665
Synonyms MGC60730
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR204864 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACTCCAGTTTTCCTCCTGGCCACCCTGTGCTTAGGGGTGGTCTCAGCTGCTCCAGCACACGATCCCA
GTTTGGATGCTGTATGGGAGGAATGGAAGACAAAACACAGGAAAACATACAGCATGAATGAAGAAGCACA
GAAGAGAGCAGTGTGGGAGAATAACATGAAGATGATCGGCCTGCACAACGAGGACTATCTGAAGGGGAAG
CATGGCTTCAACTTGGAGATGAACGCCTTTGGTGATTTGACCAATACAGAATTCAGAGAGTTGATGACTG
GCTTTCAAAGCATGGGGCACAAGGAGATGACCATCTTCCAGGAGCCCTTGCTGGGTGATGTCCCCAAGTC
TGTTGATTGGAGAGATCATGGCTATGTGACTCCTGTGAAAGACCAGGGTCATTGTGGATCTTGTTGGGCT
TTTAGTGCAGTTGGTTCCTTGGAAGGACAAATATTCAGAAAAACAGGCAAACTGGTCCCTCTGAGTGAAC
AGAACCTAATGGACTGCTCCTGGTCCTATGGCAACGTAGGTTGTAATGGTGGCTTGATGGAACTAGCCTT
CCAGTATGTGAAGGAAAACAGAGGCCTGGACACAAGGGAGTCCTACGCATATGAAGCCTGGGATGGACCC
TGCAGGTACGATCCTAAGTACTCTGCAGTTAACATCACAGGCTTTGTGAAAGTCCCATTGAGCGAAGATG
CCCTGATGAATGCCGTGGCCAGTGTGGGGCCCGTCTCAGTTGGAATTGACACTCATCACCACTCCTTCAG
GTTCTACAGAGGTGGTACGTACTATGAGCCAGACTGTAGCAGCACTAATCTGGATCATGCTGTCCTGGTA
GTTGGCTATGGTGAAGAATCAGATGGCAGGAAGTACTGGCTGGTCAAGAACAGCTGGGGTGAAGACTGGG
GCATGGATGGCTACATAAAGATGGCCAAAGACAGGGACAACAACTGTGGGATTGCTACCTATGCCATCTA
CCCCACCGTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR204864 protein sequence
Red=Cloning site Green=Tags(s)

MTPVFLLATLCLGVVSAAPAHDPSLDAVWEEWKTKHRKTYSMNEEAQKRAVWENNMKMIGLHNEDYLKGK
HGFNLEMNAFGDLTNTEFRELMTGFQSMGHKEMTIFQEPLLGDVPKSVDWRDHGYVTPVKDQGHCGSCWA
FSAVGSLEGQIFRKTGKLVPLSEQNLMDCSWSYGNVGCNGGLMELAFQYVKENRGLDTRESYAYEAWDGP
CRYDPKYSAVNITGFVKVPLSEDALMNAVASVGPVSVGIDTHHHSFRFYRGGTYYEPDCSSTNLDHAVLV
VGYGEESDGRKYWLVKNSWGEDWGMDGYIKMAKDRDNNCGIATYAIYPTV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_199148
ORF Size 990 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_199148.1, NP_954599.1
RefSeq Size 1382 bp
RefSeq ORF 993 bp
Locus ID 218275
Cytogenetics 13 B2
MW 37.1 kDa
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"NM_199148" in other vectors (4)
SKU Description Size Price
MC201883 BC051665 (untagged) - Mouse cDNA sequence BC051665 (BC051665), (10ug) 10 ug
$300.00
MG204864 BC051665 (tGFP-tagged) - Mouse cDNA sequence BC051665 (BC051665) 10 ug
$500.00
MR204864L3 Lenti ORF clone of BC051665 (Myc-DDK-tagged) - Mouse cDNA sequence BC051665 (BC051665) 10 ug
$600.00
MR204864L4 Lenti ORF clone of BC051665 (mGFP-tagged) - Mouse cDNA sequence BC051665 (BC051665) 10 ug
$600.00

Plasmid Map for MR204864

Download sequence for

file-download TXT (FASTA format)

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Cloning scheme for MR204864
Circular map for MR204864

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