Emb (NM_010330) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR204860
Emb (Myc-DDK-tagged) - Mouse embigin (Emb)
  $450.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Emb
Synonyms AL022799; Gp70
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR204860 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGCTCGCACACTGGCCTGAGGGCGCTTGTGGCGCCTGGTTACCCGCTCCTCCTTCTATGTCTTCTGG
CGGCGACGCGCCCTGATCCGGCTGAGGGCGATCCCACAGATCCAACTTTTACAAGTCTACCTGTTCGAGA
AGAAATGATGGCGAAATACTCAAACCTTTCCTTAAAGAGCTGTAATATCTCAGTGACGGAAAAGTCCAAT
GTATCAGTAGAAGAGAACGTAATTTTGGAAAAGCCTTCTCATGTGGAACTCAAATGCGTGTACACAGCAA
CTAAGGATTTGAACTTGATGAATGTGACTTGGAAGAAAGATGATGAGCCCCTTGAGACTACGGGTGACTT
CAATACAACTAAAATGGGCAACACCTTAACCAGTCAGTACAGGTTCATCGTTTTTAATAGCAAACAATTG
GGAAAATATTCTTGTGTCTTTGGAGAAAAGGAACTAAGAGGGACCTTTAACATCCACGTACCCAAAGCTC
ATGGGAAAAAAAAGTCGTTGATCGCTTACGTGGGGGATTCTACTGTGCTGAAGTGTGTATGTCAAGATTG
TCTTCCTTTAAATTGGACTTGGTACATGGGTAATGAAACCGCACAGGTTCCCATTGACGCTCACTCGAAT
GAAAAGTATATCATCAATGGTTCCCATGCCAATGAAACAAGGCTCAAGATTAAGCATCTTTTGGAGGAAG
ATGGAGGATCCTACTGGTGTCGTGCCACCTTCCAGTTAGGGGAGAGTGAGGAGCAGAATGAGCTGGTTGT
GCTGAGCTTCCTGGTGCCCCTCAAGCCATTTCTGGCCATACTTGCCGAAGTCATCCTCTTGGTGGCCATC
ATTCTGCTTTGTGAAGTGTACACACACAAGAAAAAGAATGACCCAGATGCTGGGAAAGAATTTGAACAAA
TTGAACAGCTGAAATCAGATGATAGCAATGGCATAGAAAACAATGTCCCCCGGTACAGAAAAACTGACTC
TGCAGATCAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR204860 protein sequence
Red=Cloning site Green=Tags(s)

MRSHTGLRALVAPGYPLLLLCLLAATRPDPAEGDPTDPTFTSLPVREEMMAKYSNLSLKSCNISVTEKSN
VSVEENVILEKPSHVELKCVYTATKDLNLMNVTWKKDDEPLETTGDFNTTKMGNTLTSQYRFIVFNSKQL
GKYSCVFGEKELRGTFNIHVPKAHGKKKSLIAYVGDSTVLKCVCQDCLPLNWTWYMGNETAQVPIDAHSN
EKYIINGSHANETRLKIKHLLEEDGGSYWCRATFQLGESEEQNELVVLSFLVPLKPFLAILAEVILLVAI
ILLCEVYTHKKKNDPDAGKEFEQIEQLKSDDSNGIENNVPRYRKTDSADQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_010330
ORF Size 990 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_010330.4
RefSeq Size 2622 bp
RefSeq ORF 993 bp
Locus ID 13723
UniProt ID P21995
Cytogenetics 13 D2.3
MW 37.1 kDa
Summary Plays a role in targeting the monocarboxylate transporters SLC16A1 and SLC16A7 to the cell membrane (By similarity). Plays a role in the outgrowth of motoneurons and in the formation of neuromuscular junctions. Following muscle denervation, promotes nerve terminal sprouting and the formation of additional acetylcholine receptor clusters at synaptic sites without affecting terminal Schwann cell number or morphology. Delays the retraction of terminal sprouts following re-innervation of denervated endplates.UniProtKB/Swiss-Prot Function
Reviews
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_010330" in other vectors (4)
SKU Description Size Price
MC208434 Emb (untagged) - Mouse embigin (Emb), (10ug) 10 ug
$450.00
MG204860 Emb (tGFP-tagged) - Mouse embigin (Emb) 10 ug
$650.00
MR204860L3 Lenti ORF clone of Emb (Myc-DDK-tagged) - Mouse embigin (Emb) 10 ug
$750.00
MR204860L4 Lenti ORF clone of Emb (mGFP-tagged) - Mouse embigin (Emb) 10 ug
$750.00

Plasmid Map for MR204860

Download sequence for

Cloning scheme for MR204860

Circular map for MR204860

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products