Cnn3 (NM_028044) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR204859
Cnn3 (Myc-DDK-tagged) - Mouse calponin 3, acidic (Cnn3)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cnn3
Synonyms 1600014M03Rik; C85854; Calpo3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR204859 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCCACTTCAACAAGGGCCCTTCCTACGGGCTCTCCGCCGAAGTTAAGAACAAGATTGCATCCAAGT
ATGACCAGCAGGCCGAGGAAGATCTGCGCAACTGGATAGAAGAGGTGACAGGCCTAGGCATTGGCACCAA
CTTCCAGCTGGGGCTGAAGGACGGCATCATATTGTGCGAACTCATAAACAAGCTACAGCCAGGCTCTGTG
AAGAAAGTCAACGAATCCTCACTAAATTGGCCCCAGTTGGAGAATATCGGCAACTTCATTAAAGCTATCC
AGGCTTACGGTATGAAGCCCCATGATATATTTGAAGCAAACGACCTCTTTGAGAATGGCAACATGACCCA
GGTTCAGACGACGCTGGTGGCTCTAGCAGGTCTGGCGAAAACAAAAGGATTCCATACAACCATTGACATT
GGCGTTAAGTATGCAGAAAAACAAACAAGACGTTTTGATGAAGGCAAATTAAAGGCTGGCCAGAGTGTAA
TTGGTTTACAGATGGGTACCAACAAATGTGCCAGCCAGGCGGGCATGACAGCCTATGGGACTCGGAGGCA
TCTTTATGATCCCAAGATGCAGACGGACAAACCCTTTGACCAGACCACGATTAGCCTGCAGATGGGCACC
AACAAAGGGGCCAGCCAGGCTGGGATGTTAGCACCGGGCACCAGAAGAGACATCTATGACCAGAAGCTGA
CATTACAGCCAGTGGACAACTCGACCATTTCTCTACAGATGGGCACCAACAAAGTTGCTTCCCAGAAAGG
AATGAGCGTGTATGGGCTTGGGCGGCAAGTATATGACCCCAAGTACTGTGCCGCACCCACAGAACCTGTC
ATTCACAACGGAAGCCAGGGCACGGGCACCAATGGGTCGGAAATCAGTGATAGCGATTATCAGGCAGAAT
ACCCCGATGAATATCATGGCGAGTACCCAGACGACTACCCTCGGGAGTACCAGTATGGCGACGACCAGGG
CATTGATTAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR204859 protein sequence
Red=Cloning site Green=Tags(s)

MTHFNKGPSYGLSAEVKNKIASKYDQQAEEDLRNWIEEVTGLGIGTNFQLGLKDGIILCELINKLQPGSV
KKVNESSLNWPQLENIGNFIKAIQAYGMKPHDIFEANDLFENGNMTQVQTTLVALAGLAKTKGFHTTIDI
GVKYAEKQTRRFDEGKLKAGQSVIGLQMGTNKCASQAGMTAYGTRRHLYDPKMQTDKPFDQTTISLQMGT
NKGASQAGMLAPGTRRDIYDQKLTLQPVDNSTISLQMGTNKVASQKGMSVYGLGRQVYDPKYCAAPTEPV
IHNGSQGTGTNGSEISDSDYQAEYPDEYHGEYPDDYPREYQYGDDQGIDY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_028044
ORF Size 990 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028044.2, NP_082320.1
RefSeq Size 2003 bp
RefSeq ORF 993 bp
Locus ID 71994
UniProt ID Q9DAW9
Cytogenetics 3 G1
MW 36.4 kDa
Summary Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_028044" in other vectors (4)
SKU Description Size Price
MC207711 Cnn3 (untagged) - Mouse calponin 3, acidic (Cnn3), (10ug) 10 ug
$330.00
MG204859 Cnn3 (tGFP-tagged) - Mouse calponin 3, acidic (Cnn3) 10 ug
$500.00
MR204859L3 Lenti ORF clone of Cnn3 (Myc-DDK-tagged) - Mouse calponin 3, acidic (Cnn3) 10 ug
$600.00
MR204859L4 Lenti ORF clone of Cnn3 (mGFP-tagged) - Mouse calponin 3, acidic (Cnn3) 10 ug
$600.00

Plasmid Map for MR204859

Download sequence for

Cloning scheme for MR204859

Circular map for MR204859

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