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Pdlim4 (NM_019417) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR204849
Pdlim4 (Myc-DDK-tagged) - Mouse PDZ and LIM domain 4 (Pdlim4)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pdlim4
Synonyms Ril
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR204849 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCCACTCGGTGACCCTGCGCGGCCCTTCACCCTGGGGCTTCCGCCTGGTGGGCGGCCGGGACTTCA
GTGCTCCCCTCACCATCTCGCGGGTTCATGCTGGGAGCAAAGCGGCCCTGGCTGCCCTGTGCCCAGGTGA
CTTGATCCAAGCCATCAACGGCGAGAGCACTGAGCTCATGACACACCTGGAGGCCCAGAACCGCATTAAA
GGCTGCCACGATCATCTTACGCTCTCGGTCAGCAGGCCTGAGAATAAGAACTGGCCCAGTGCTCCTGATG
ACAAGGCTCAAGCACATAGGATCCACATTGACCCTGAGTCCCAGGACTGCAGTCCAGCCACCAGCAGGCG
CTCCTCAGTCTCTGGGATTAGTCTGGAGGACAACAGATCGGGCCTGGGATCTCCATATGGTCAGCCACCT
CGCCTTCCAGTCCCTCACAATGGCAGCAGCAACGAGGCCACCCTGCCAGCCCAGATGAGCGCTCTGCATG
TGTCTCCACCCACCAGTGCCGACACAGCCAGGGTTCTCCCTCGGAACCGGGACTGCAGGGTAGACCTGGG
TTCAGAGGTATACAGGATGTTAAGGGAGCCAGCAGAGCCCACAGCCTCGGAGCCGAAACAGTCAGGATCC
TTCCGCTACCTGCAGGGCATGCTAGAGGCTGGCGAGGGCGGGGATCGGCCTGGGTCTGGTGGTCCCCGGA
ACCTCAAACCAGCAGCCAGCAAGCTCGGTGCTCCACTGAGTGGCCTGCAGGGGCTACCAGAGTGCACGCG
CTGCGGCCACGGGATCGTGGGAACCATTGTCAAGGCGAGAGACAAGCTCTACCATCCGGAGTGCTTCATG
TGCAGCGACTGCGGCCTGAATCTCAAACAGCGTGGGTACTTCTTCCTAGATGAACGGCTGTACTGTGAGA
ATCACGCCAAGGCTCGAGTCAAGCCGCCGGAGGGATATGATGTGGTGGCTGTATACCCCAATGCTAAGGT
GGAACTTGTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR204849 protein sequence
Red=Cloning site Green=Tags(s)

MTHSVTLRGPSPWGFRLVGGRDFSAPLTISRVHAGSKAALAALCPGDLIQAINGESTELMTHLEAQNRIK
GCHDHLTLSVSRPENKNWPSAPDDKAQAHRIHIDPESQDCSPATSRRSSVSGISLEDNRSGLGSPYGQPP
RLPVPHNGSSNEATLPAQMSALHVSPPTSADTARVLPRNRDCRVDLGSEVYRMLREPAEPTASEPKQSGS
FRYLQGMLEAGEGGDRPGSGGPRNLKPAASKLGAPLSGLQGLPECTRCGHGIVGTIVKARDKLYHPECFM
CSDCGLNLKQRGYFFLDERLYCENHAKARVKPPEGYDVVAVYPNAKVELV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_019417
ORF Size 990 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019417.3
RefSeq Size 1189 bp
RefSeq ORF 993 bp
Locus ID 30794
UniProt ID P70271
Cytogenetics 11 32.13 cM
MW 35.6 kDa
Summary Suppresses SRC activation by recognizing and binding to active SRC and facilitating PTPN13-mediated dephosphorylation of SRC 'Tyr-419' leading to its inactivation. Inactivated SRC dissociates from this protein allowing the initiation of a new SRC inactivation cycle. Involved in reorganization of the actin cytoskeleton (By similarity). In nonmuscle cells, binds to ACTN1 (alpha-actinin-1), increases the affinity of ACTN1 to F-actin (filamentous actin), and promotes formation of actin stress fibers. Involved in regulation of the synaptic AMPA receptor transport in dendritic spines of hippocampal pyramidal neurons directing the receptors toward an insertion at the postsynaptic membrane. Links endosomal surface-internalized GRIA1-containing AMPA receptors to the alpha-actinin/actin cytoskeleton. Increases AMPA receptor-mediated excitatory postsynaptic currents in neurons (By similarity).UniProtKB/Swiss-Prot Function
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"NM_019417" in other vectors (4)
SKU Description Size Price
MC204696 Pdlim4 (untagged) - Mouse PDZ and LIM domain 4 (Pdlim4), (10ug) 10 ug
$300.00
MG204849 Pdlim4 (tGFP-tagged) - Mouse PDZ and LIM domain 4 (Pdlim4) 10 ug
$500.00
MR204849L3 Lenti ORF clone of Pdlim4 (Myc-DDK-tagged) - Mouse PDZ and LIM domain 4 (Pdlim4) 10 ug
$600.00
MR204849L4 Lenti ORF clone of Pdlim4 (mGFP-tagged) - Mouse PDZ and LIM domain 4 (Pdlim4) 10 ug
$600.00

Plasmid Map for MR204849

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR204849
Circular map for MR204849

file-alt Citations

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