Rad51d (NM_011235) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR204841
Rad51d (Myc-DDK-tagged) - Mouse RAD51-like 3 (S. cerevisiae) (Rad51l3)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rad51d
Synonyms R51H; R51H3; Rad5; Rad51l3; TRAD; Trad-d5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR204841 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCATGCTCAGGGCAGGGCTGTGCCCGGGCCTCACCGAGGAGACCGTCCAGCTTCTCAGAGGCCGAA
AGATAAAAACAGTGGCAGACCTGGCAGCTGCTGACTTGGAGGAAGTAGCCCAGAAGTGTGGCTTGTCCTA
CAAGGCCCTCGTTGCCCTGAGGAGGGTGTTGCTGGCGCAGTTCTCGGCTTTCCCCTTAAATGGCGCAGAT
CTCTATGAGGAACTGAAGACTTCCACGGCCATCCTGTCCACCGGCATCGGAAGCCTGGACAAACTACTTG
ATGCTGGCCTCTATACTGGGGAGGTGACTGAAATTGTGGGTGGCCCAGGTAGCGGCAAAACCCAGGTGTG
TCTCTGTGTGGCTGCAAATGTGGCCCATAGCCTGCAGCAGAATGTACTGTATGTGGATTCCAATGGAGGA
ATGACGGCGTCCCGCCTCCTCCAGCTACTACAGGCTAGAACCCAAGATGAGGAGAAACAGGCAAGTGCTC
TCCAGAGGATACAGGTGGTGCGTTCATTTGACATCTTCCGGATGCTAGATATGCTACAGGACCTTCGCGG
CACCATAGCCCAGCAGGAAGCAACTTCTTCAGGCGCCGTGAAGGTTGTGATTGTGGACTCGGTCACTGCA
GTGGTCGCCCCACTTCTGGGAGGTCAGCAGAGGGAAGGCCTGGCCTTGATGATGCAGCTGGCCCGAGAGC
TCAAGATCCTGGCCCGGGACCTGGGTGTGGCAGTGGTGGTGACCAACCACTTGACTCGAGATTGGGATGG
TAGAAGATTCAAACCTGCCCTTGGACGCTCCTGGAGCTTTGTGCCCAGTACCCGGATTCTCCTGGATGTC
ACTGAGGGGGCTGGGACACTCGGTAGCAGCCAACGCACAGTATGTCTGACCAAGTCTCCCCGCCAGCCAA
CGGGTCTGCAGGAGATGATAGACATTGGGACATTGGGGACTGAGGAGCAGAGCCCAGAATTACCTGGCAA
GCAGACG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR204841 protein sequence
Red=Cloning site Green=Tags(s)

MGMLRAGLCPGLTEETVQLLRGRKIKTVADLAAADLEEVAQKCGLSYKALVALRRVLLAQFSAFPLNGAD
LYEELKTSTAILSTGIGSLDKLLDAGLYTGEVTEIVGGPGSGKTQVCLCVAANVAHSLQQNVLYVDSNGG
MTASRLLQLLQARTQDEEKQASALQRIQVVRSFDIFRMLDMLQDLRGTIAQQEATSSGAVKVVIVDSVTA
VVAPLLGGQQREGLALMMQLARELKILARDLGVAVVVTNHLTRDWDGRRFKPALGRSWSFVPSTRILLDV
TEGAGTLGSSQRTVCLTKSPRQPTGLQEMIDIGTLGTEEQSPELPGKQT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_011235
ORF Size 987 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_011235.4, NP_035365.1
RefSeq Size 7072 bp
RefSeq ORF 990 bp
Locus ID 19364
UniProt ID O55230
Cytogenetics 11 50.3 cM
MW 35.3 kDa
Summary This gene belongs to the Rad51 gene family whose products play a major role in homologous recombination and DNA repair. The encoded protein interacts with other proteins of this family, including Rad51b, Rad51c and Xrcc2, and plays an essential role in both DNA repair and telomere maintenance. In humans, germline mutations in this gene may be associated with predisposition to ovarian cancer. Alternative splicing of this gene results in multiple transcript variants. provided by RefSeq, May 2013
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"NM_011235" in other vectors (4)
SKU Description Size Price
MC207374 Rad51d (untagged) - Mouse RAD51-like 3 (S. cerevisiae) (Rad51l3), (10ug) 10 ug
$330.00
MG204841 Rad51d (tGFP-tagged) - Mouse RAD51-like 3 (S. cerevisiae) (Rad51l3) 10 ug
$500.00
MR204841L3 Lenti ORF clone of Rad51d (Myc-DDK-tagged) - Mouse RAD51-like 3 (S. cerevisiae) (Rad51l3) 10 ug
$600.00
MR204841L4 Lenti ORF clone of Rad51d (mGFP-tagged) - Mouse RAD51-like 3 (S. cerevisiae) (Rad51l3) 10 ug
$600.00

Plasmid Map for MR204841

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Cloning scheme for MR204841

Circular map for MR204841

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