Psrc1 (NM_019976) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR204837
Psrc1 (Myc-DDK-tagged) - Mouse proline/serine-rich coiled-coil 1 (Psrc1), transcript variant 2
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Psrc1
Synonyms 5430413I02Rik; AL033312; Dda3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR204837 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGATCTGAAAGAGGATATCAAGTTCATTGTGGACGAGACCTTGGACTTCGGAGGGCTGTCTCCAT
CTGACAGTCATGAGGAAGAAGACATAACAGTATTAGTGAGTCCAGAGAAACCACTTCGACGGGGCCTCGC
CCATCGGAGTAACCCAAATGAAGTAGCTCCCGCCCTCCAGGGTGTGCGGTTTAGCTTGGGCCCGCTCAGC
CCAGAGAAGCTGGAAGAGATTCTTGATGAAGCCAACCGCCTGGCGGCTCAGCTGGAGGAGTGTGCCCTGA
AAGATCGGGAGAGGGCTGGTACAGGCCCTGGAAGGCCCAGCCCCAGAGGGAAACCCAGTCCTCGGCGGGA
GACCTTCGTCCTGAAGGATAGCCCTGTCCGAGATCTGCTGCCCACCGTGAGTTCTTGGAGCACCCCACCT
CCAAGCAGCCTAGCTGGGCTCCGGAGCAGTGATAAAAAGGGGTCAGCCAGGGCTGTCCGGGTGGCATCCG
GAAAGAAGCCCTCCAGCATAAAGAAGGAATCACCCACTTGCAATCTGTTCCCTGCATCCAAAAGCCCGGG
GCGCTCTCCTCTTGCACAACCAATTCTTCCACCTCGGCGGAAAACTGGGTTCGGTGCCCGGACAACAGCA
AGCCCACCAATTCCTGTCAGACCAGTTCCACAGTCCTCAGCTAGCAACTCCCAATGTTCATCCCGGCTCC
AGGGAGCAGCTGTCAAGTCTTCCAGTCGACTCCCTGTCCCTTCAGCCATCCCCAAGCCTGCCACCCGAGT
GCCACTCATTGGGCGGAGTCTACCACCTGGAAAAGGTGCCCTAGCTCCAGATTCTCTCTCAACTCAGAAA
GGGCATCCAAGCGCCATAGGGCACAGAGCCTCTGTTTCCCAGAAAACAAACCTTCCAACCACCAGTGCGG
CTCGAGGCAGGACCACCAGTGCCGCTCGAGGCAGGGCGCAGCCCCTCAGGAAAGCTGCAGTCCCTGGACC
GACTAGG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR204837 protein sequence
Red=Cloning site Green=Tags(s)

MEDLKEDIKFIVDETLDFGGLSPSDSHEEEDITVLVSPEKPLRRGLAHRSNPNEVAPALQGVRFSLGPLS
PEKLEEILDEANRLAAQLEECALKDRERAGTGPGRPSPRGKPSPRRETFVLKDSPVRDLLPTVSSWSTPP
PSSLAGLRSSDKKGSARAVRVASGKKPSSIKKESPTCNLFPASKSPGRSPLAQPILPPRRKTGFGARTTA
SPPIPVRPVPQSSASNSQCSSRLQGAAVKSSSRLPVPSAIPKPATRVPLIGRSLPPGKGALAPDSLSTQK
GHPSAIGHRASVSQKTNLPTTSAARGRTTSAARGRAQPLRKAAVPGPTR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_019976
ORF Size 987 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019976.3
RefSeq Size 1739 bp
RefSeq ORF 990 bp
Locus ID 56742
UniProt ID Q9D0P7
Cytogenetics 3 F3
MW 34.7 kDa
Summary Required for normal progression through mitosis. Required for normal congress of chromosomes at the metaphase plate, and for normal rate of chromosomal segregation during anaphase. Plays a role in the regulation of mitotic spindle dynamics. Increases the rate of turnover of microtubules on metaphase spindles, and contributes to the generation of normal tension across sister kinetochores. Recruits KIF2A and ANKRD53 to the mitotic spindle and spindle poles. May participate in p53/TP53-regulated growth suppression (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_019976" in other vectors (4)
SKU Description Size Price
MC205367 Psrc1 (untagged) - Mouse proline/serine-rich coiled-coil 1 (Psrc1), transcript variant 2, (10ug) 10 ug
$300.00
MG204837 Psrc1 (tGFP-tagged) - Mouse proline/serine-rich coiled-coil 1 (Psrc1) 10 ug
$500.00
MR204837L3 Lenti ORF clone of Psrc1 (Myc-DDK-tagged) - Mouse proline/serine-rich coiled-coil 1 (Psrc1), transcript variant 2 10 ug
$600.00
MR204837L4 Lenti ORF clone of Psrc1 (mGFP-tagged) - Mouse proline/serine-rich coiled-coil 1 (Psrc1), transcript variant 2 10 ug
$600.00

Plasmid Map for MR204837

Download sequence for

Cloning scheme for MR204837

Circular map for MR204837

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