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5730593N15Rik (BC025832) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR204813
5730593N15Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 5730593N15 gene (cDNA clone MGC:37936 IMAGE:5125723)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "5730593N15Rik or BC025832"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol 5730593N15Rik
Synonyms 5730593N15Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR204813 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGGGCTCACCTGATCCCTGTTACAGCTTCATCCCTTACTGCTCCAGTGACGTTTGGAGTGGGGCTT
CACCCAAGTCTGACAAGAATGAATATGCCTTCATGGGCTCCCTCATCATCCAGGAGGTGGTACGGGAGCT
CCTGGGCAAAGGGCTGAGCGGGGCAAAGGTGCTGCTGTTGGCCGGGAGCAGCGCTGGGGGCACCGGGGTG
CTGTTGAATGTAGACCGCGTGGCTGAGCTGCTGGAGGAGTTGGGCTACCCGTCTATCCAGGTGCGGGGCC
TGGCTGACTCAGGCTGGTTCCTGGACAACAAGCAGTACCGGCGATCAGACTGCATTGATACTATCAACTG
TGCACCCACAGACGCCATCCGCCGTGGCATCAGGTACTGGAGTGGGATGGTCCCAGAGCGTTGCCAGCGC
CAGTTCAAGGAAGGAGAGGAATGGAATTGCTTCTTTGGCTACAAAGTATATCCGACTCTGCGCTGTCCGG
TGTTCGTGGTACAGTGGCTTTTCGATGAGGCCCAGCTTACCGTGGATAACGTGCATCTCACCGGACAGCC
GGTTCAGGAAGGCCAGTGGCTATACATCCAGAATCTGGGCCGAGAACTGCGTGGTACACTCAAGGATGTG
CAGGCCAGCTTTGCCCCCGCCTGCCTGTCTCATGAGATCATCATACGGAGCTATTGGACGGACGTCCAGG
TGAAGGGGACCTCACTGCCCAGGGCACTGCACTGCTGGGACCGGAGCTTCCATGACAGCCATAAGGCCAG
CAAAACCCCCATGAAGGGCTGCCCTTTCCACCTGGTAGACAGCTGCCCCTGGCCCCACTGCAATCCCTCA
TGCCCTACCATCCGGGACCAGTTCACAGGGCAAGAGATGAACGTGGCACAGTTCCTTATGCATATGGGCT
TCGATGTGCAGATGGTGGCTCAGCAGCAGGGGATGGAGCCCAGCAAGCTGCTAGGGATGCTGAGTAATGG
GAAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR204813 protein sequence
Red=Cloning site Green=Tags(s)

MVGSPDPCYSFIPYCSSDVWSGASPKSDKNEYAFMGSLIIQEVVRELLGKGLSGAKVLLLAGSSAGGTGV
LLNVDRVAELLEELGYPSIQVRGLADSGWFLDNKQYRRSDCIDTINCAPTDAIRRGIRYWSGMVPERCQR
QFKEGEEWNCFFGYKVYPTLRCPVFVVQWLFDEAQLTVDNVHLTGQPVQEGQWLYIQNLGRELRGTLKDV
QASFAPACLSHEIIIRSYWTDVQVKGTSLPRALHCWDRSFHDSHKASKTPMKGCPFHLVDSCPWPHCNPS
CPTIRDQFTGQEMNVAQFLMHMGFDVQMVAQQQGMEPSKLLGMLSNGN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC025832
ORF Size 984 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC025832, AAH25832
RefSeq Size 1447 bp
RefSeq ORF 986 bp
Locus ID 77583
Cytogenetics 11 E2
MW 36.8 kDa
Summary Carboxylesterase that acts as a key negative regulator of the Wnt signaling pathway by specifically mediating depalmitoleoylation of WNT proteins. Serine palmitoleoylation of WNT proteins is required for efficient binding to frizzled receptors.UniProtKB/Swiss-Prot Function
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"BC025832" in other vectors (4)
SKU Description Size Price
MC217920 5730593N15Rik (untagged) - Mouse RIKEN cDNA 5730593N15 gene (cDNA clone MGC:37936 IMAGE:5125723), (10ug) 10 ug
$330.00
MG204813 5730593N15Rik (tGFP-tagged) - Mouse RIKEN cDNA 5730593N15 gene (cDNA clone MGC:37936 IMAGE:5125723) 10 ug
$500.00
MR204813L3 Lenti ORF clone of 5730593N15Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 5730593N15 gene (cDNA clone MGC:37936 IMAGE:5125723) 10 ug
$600.00
MR204813L4 Lenti ORF clone of 5730593N15Rik (mGFP-tagged) - Mouse RIKEN cDNA 5730593N15 gene (cDNA clone MGC:37936 IMAGE:5125723) 10 ug
$600.00

Plasmid Map for MR204813

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR204813
Circular map for MR204813

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