Ppp1cb (NM_172707) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR204795
Ppp1cb (Myc-DDK-tagged) - Mouse protein phosphatase 1, catalytic subunit, beta isoform (Ppp1cb)
  $450.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ppp1cb
Synonyms 1200010B19
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR204795 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGACGGGGAGCTGAACGTGGACAGCCTCATCACCCGCCTGCTGGAGGTACGAGGATGTCGTCCAG
GAAAGATTGTACAAATGACTGAAGCAGAAGTCCGAGGGTTGTGTATCAAGTCTCGTGAAATCTTTCTCAG
CCAGCCTATTCTTTTGGAATTGGAAGCACCACTGAAGATTTGTGGAGACATTCATGGACAATATACAGAC
TTACTAAGATTATTTGAATATGGAGGTTTTCCACCAGAAGCCAACTATCTTTTCTTAGGAGATTATGTGG
ACAGAGGAAAGCAGTCTTTGGAAACCATCTGTTTGCTATTGGCTTACAAAATCAAATACCCGGAGAACTT
CTTTCTTCTAAGAGGAAACCATGAGTGTGCTAGCATCAATCGCATTTATGGATTCTACGATGAGTGTAAA
CGAAGATTTAATATTAAATTGTGGAAGACATTCACTGATTGTTTTAACTGTCTGCCTATAGCTGCTATTG
TTGATGAGAAAATCTTCTGTTGTCATGGAGGACTGTCACCAGACCTACAATCTATGGAACAGATTCGGAG
AATTATGAGACCCACTGACGTACCTGATACAGGTTTGCTTTGTGACTTACTGTGGTCCGACCCAGATAAG
GATGTGCAAGGCTGGGGAGAAAATGACCGTGGTGTTTCTTTTACTTTTGGAGCTGATGTAGTCAGTAAAT
TTCTGAATCGTCATGATTTAGACTTGATTTGTCGAGCTCATCAGGTGGTGGAAGACGGATATGAATTTTT
TGCTAAACGACAGTTGGTAACCTTATTTTCTGCCCCAAATTACTGTGGCGAGTTTGACAATGCTGGTGGT
ATGATGAGTGTGGATGAGACTTTGATGTGTTCATTCCAGATATTGAAACCATCTGAAAAGAAAGCTAAGT
ACCAGTATGGTGGGCTGAATTCTGGACGTCCTGTCACTCCGCCTCGAACAGCTAATCCACCGAAGAAAAG
G


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR204795 protein sequence
Red=Cloning site Green=Tags(s)

MADGELNVDSLITRLLEVRGCRPGKIVQMTEAEVRGLCIKSREIFLSQPILLELEAPLKICGDIHGQYTD
LLRLFEYGGFPPEANYLFLGDYVDRGKQSLETICLLLAYKIKYPENFFLLRGNHECASINRIYGFYDECK
RRFNIKLWKTFTDCFNCLPIAAIVDEKIFCCHGGLSPDLQSMEQIRRIMRPTDVPDTGLLCDLLWSDPDK
DVQGWGENDRGVSFTFGADVVSKFLNRHDLDLICRAHQVVEDGYEFFAKRQLVTLFSAPNYCGEFDNAGG
MMSVDETLMCSFQILKPSEKKAKYQYGGLNSGRPVTPPRTANPPKKR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_172707
ORF Size 981 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_172707.3
RefSeq Size 4024 bp
RefSeq ORF 984 bp
Locus ID 19046
UniProt ID P62141
Cytogenetics 5 B1
MW 37.2 kDa
Summary Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase (PP1) is essential for cell division, it participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. In balance with CSNK1D and CSNK1E, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. May dephosphorylate CSNK1D and CSNK1E.UniProtKB/Swiss-Prot Function
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"NM_172707" in other vectors (4)
SKU Description Size Price
MC202635 Ppp1cb (untagged) - Mouse protein phosphatase 1, catalytic subunit, beta isoform (Ppp1cb), (10ug) 10 ug
$450.00
MG204795 Ppp1cb (tGFP-tagged) - Mouse protein phosphatase 1, catalytic subunit, beta isoform (Ppp1cb) 10 ug
$650.00
MR204795L3 Lenti ORF clone of Ppp1cb (Myc-DDK-tagged) - Mouse protein phosphatase 1, catalytic subunit, beta isoform (Ppp1cb) 10 ug
$750.00
MR204795L4 Lenti ORF clone of Ppp1cb (mGFP-tagged) - Mouse protein phosphatase 1, catalytic subunit, beta isoform (Ppp1cb) 10 ug
$750.00

Plasmid Map for MR204795

Download sequence for

Cloning scheme for MR204795

Circular map for MR204795

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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