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Apof (NM_133997) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR204777
Apof (Myc-DDK-tagged) - Mouse apolipoprotein F (Apof)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Apof
Synonyms AI255964
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR204777 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGGACTGTATGGGTGCTCAGCTCCTGACATGCATAGCCTCCGACTCATCCTGATGTCAATCCAGC
TGCTTTGCTATCTCCTGCTGTGTCCTGTGGATGCCACTTCACACGGAGAGGCAACCAGTGTCTCTACGGT
GCTCCCTCCCTCGAAGTTGGGATACCAGATGCAGACCTCAGACAAGCCCTTGTCCTGCCAGATGCTGCTC
CCCAAGTCCCTGCCTGGCTTCACTTACATGCCCCCTGTCTCCAAATTCCTGGTAGGCCTGGCTCTAAGGA
ATGCTCTGGAGGCAGCTGGCTGCCAGGCTGAGGTCTGGGCTCTTCAGCTTCAGCTTTACCGATTAGGAGG
TGTGGAGGCTACACAAGCCCTCATCCACCATCTCCAGGAGCTCCAGAAGAGTGGACACACAGACCGGGAA
GTGTCTGTGGATGCCCTGTCCTCTGCTCTGCAGCTCTTAGCCTGGGAGCAGCCAGGTCCAAAGAGGGCCA
AACGTTCGATTTCCAACACAGACTGCGACAATGATCAGGAACAGAGTGTCCACAATGTGGTTGACCTACT
ACCAGCAGTAGGCACCTACTACAACCTAGGCACAGCTTTGTATTACGCCATTAAGAACTGCTCTGACAAG
GCCAAGGAACGAGGTCGAGATGGGGCCATAGATCTGGGTTATGATCTCTTGATGGCCATGGTAGGCGCGT
CAGGGGGCCCAGCTGGTGCGGTGATCACGGCTGCTCTTAAGCCAGCGATGAAGGCTGGGGTTCAGCGGCT
GATCCAGTATTATTATGATGAGAAAGAGGTGACCACCCCTCAGCCAGAGGTGACCACCCATCAGCCAGAG
ACCGGAAAGGATGCCACCACAGATATCGGTGTTGTGGAAGAAATAGCCATGTCTAATTTTGTGTCAGAAG
TTGAAAGTACAACTTCTAACTGGGAGTGGCCTTTATTAAAGAACTATGGTGTCTTGGCGTACAAGAGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR204777 protein sequence
Red=Cloning site Green=Tags(s)

MAGLYGCSAPDMHSLRLILMSIQLLCYLLLCPVDATSHGEATSVSTVLPPSKLGYQMQTSDKPLSCQMLL
PKSLPGFTYMPPVSKFLVGLALRNALEAAGCQAEVWALQLQLYRLGGVEATQALIHHLQELQKSGHTDRE
VSVDALSSALQLLAWEQPGPKRAKRSISNTDCDNDQEQSVHNVVDLLPAVGTYYNLGTALYYAIKNCSDK
AKERGRDGAIDLGYDLLMAMVGASGGPAGAVITAALKPAMKAGVQRLIQYYYDEKEVTTPQPEVTTHQPE
TGKDATTDIGVVEEIAMSNFVSEVESTTSNWEWPLLKNYGVLAYKR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_133997
ORF Size 978 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_133997.2, NP_598758.1
RefSeq Size 1930 bp
RefSeq ORF 981 bp
Locus ID 103161
UniProt ID Q91V80
Cytogenetics 10 76.5 cM
MW 35.4 kDa
Summary Minor apolipoprotein that associates with LDL. Inhibits cholesteryl ester transfer protein (CETP) activity and appears to be an important regulator of cholesterol transport. Also associates to a lesser degree with VLDL, Apo-AI and Apo-AII.UniProtKB/Swiss-Prot Function
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"NM_133997" in other vectors (4)
SKU Description Size Price
MC201962 Apof (untagged) - Mouse apolipoprotein F (Apof), (10ug) 10 ug
$300.00
MG204777 Apof (tGFP-tagged) - Mouse apolipoprotein F (Apof) 10 ug
$500.00
MR204777L3 Lenti ORF clone of Apof (Myc-DDK-tagged) - Mouse apolipoprotein F (Apof) 10 ug
$600.00
MR204777L4 Lenti ORF clone of Apof (mGFP-tagged) - Mouse apolipoprotein F (Apof) 10 ug
$600.00

Plasmid Map for MR204777

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR204777
Circular map for MR204777

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