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Pim3 (NM_145478) Mouse Tagged Lenti ORF Clone

  • LentiORF®
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SKU
MR204756L4
Lenti ORF clone of Pim3 (mGFP-tagged) - Mouse proviral integration site 3 (Pim3)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
mGFP tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $600.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pim3
Synonyms BC026639; Kid1
Vector pLenti-C-mGFP-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(MR204756).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_145478
ORF Size 978 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_145478.2, NP_663453.1
RefSeq Size 2419 bp
RefSeq ORF 981 bp
Locus ID 223775
UniProt ID P58750
Cytogenetics 15 E3
Summary Proto-oncogene with serine/threonine kinase activity that can prevent apoptosis and promote cell survival and protein translation. May contribute to tumorigenesis through: the delivery of survival signaling through phosphorylation of BAD which induces release of the anti-apoptotic protein Bcl-X(L), the regulation of cell cycle progression and protein synthesis and by regulation of MYC transcriptional activity. Additionally to this role on tumorigenesis, can also negatively regulate insulin secretion by inhibiting the activation of MAPK1/3 (ERK1/2), through SOCS6. Involved also in the control of energy metabolism and regulation of AMPK activity in modulating MYC and PPARGC1A protein levels and cell growth.UniProtKB/Swiss-Prot Function
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"NM_145478" in other vectors (4)
SKU Description Size Price
MC205349 Pim3 (untagged) - Mouse proviral integration site 3 (Pim3), (10ug) 10 ug
$300.00
MG204756 Pim3 (tGFP-tagged) - Mouse proviral integration site 3 (Pim3) 10 ug
$500.00
MR204756 Pim3 (Myc-DDK-tagged) - Mouse proviral integration site 3 (Pim3) 10 ug
$289.00 $300.00
MR204756L3 Lenti ORF clone of Pim3 (Myc-DDK-tagged) - Mouse proviral integration site 3 (Pim3) 10 ug
$600.00

Plasmid Map for MR204756L4

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Cloning scheme for MR204756L4
Circular map for MR204756L4

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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