Echdc1 (NM_025855) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR204651
Echdc1 (Myc-DDK-tagged) - Mouse enoyl Coenzyme A hydratase domain containing 1 (Echdc1), transcript variant 1
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Echdc1
Synonyms 1700028A24Rik; AI314462; AI930038; D10Ertd667e; MMCD
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR204651 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGAAGGTGTGAGGTCAATTCTAAGCCGATTTCTGAATATTTTGGAATCCCTTGTGAGAACCGAGAAA
TGGCAAAATGTCTTTTGACTTCATCTCTGTCTGTAAGAACAAAACTCTTACAAACAGGAGTGTCGCTTTA
TAATACGAGTCATGGCTTCCACGAGGAGGAAGTGAAGAAGATACTGGAGCAGTTTCCTGGTGGATCCATT
GACCTCCTAAAGAAGCAGAACGGCATTGGCATTCTGACGCTAAACAACCCCAATAAAATGAATGCCTTCT
CAGGTGTCATGATGCTACAACTTTTGGAAAGGGTAATTGAATTAGAAAATTGGACAGAAGGGAAAGGCCT
CATTATCCATGGAGCAAAAAATACTTTCTGCTCAGGATCTGATCTGAATGCTGTGAAGGCACTCTCCACT
CCAGAAAGTGGAGTGGCCTTATCTATGTTCATGCAAAACACGTTAACAAGATTTATGAGACTACCGTTAA
TAAGTGTTGCTCTGGTTCAAGGCTGGGCAATGGGTGGAGGAGCAGAACTTACTACAGCATGTGATTTCAG
GTTGATGACTGAGGAAAGTGTGATCAGATTTGTCCACAAGGAGATGGGTATAGTTCCAAGCTGGGGTGGT
ACCAGCCGTCTGGTTGAAATCATTGGCAGCAGACAAGCTCTCAAAGTGTTAAGTGGGACTCTCAAACTGG
ATTCTAAGGAAGCTTTAAATATAGGCTTGACTGATGAGGTCTTACAGCCCTCAGATGAAACTACGGCTCT
AGAACAGGCACAAGAGTGGCTGGAGAAATTTGTCAGTGGGCCTCCACAAGTCATTAGGGGTTTAAAAAAA
TCTGTCTGCTCTGCCCGAGAACTGTATATAGAGGAGGCGTTACAGAATGAACGAGACGTTTTGGAAACAC
TGTGGGGTGGACCTGCAAATTTAGAGGCTATTGCTAAGAAAGGAAAGCATACTAAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR204651 protein sequence
Red=Cloning site Green=Tags(s)

MRRCEVNSKPISEYFGIPCENREMAKCLLTSSLSVRTKLLQTGVSLYNTSHGFHEEEVKKILEQFPGGSI
DLLKKQNGIGILTLNNPNKMNAFSGVMMLQLLERVIELENWTEGKGLIIHGAKNTFCSGSDLNAVKALST
PESGVALSMFMQNTLTRFMRLPLISVALVQGWAMGGGAELTTACDFRLMTEESVIRFVHKEMGIVPSWGG
TSRLVEIIGSRQALKVLSGTLKLDSKEALNIGLTDEVLQPSDETTALEQAQEWLEKFVSGPPQVIRGLKK
SVCSARELYIEEALQNERDVLETLWGGPANLEAIAKKGKHTK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025855
ORF Size 966 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025855.1, NM_025855.2, NM_025855.3, NM_025855.4, NP_080131.3
RefSeq Size 3048 bp
RefSeq ORF 969 bp
Locus ID 52665
UniProt ID Q9D9V3
Cytogenetics 10 16.59 cM
MW 35.5 kDa
Summary Decarboxylases ethylmalonyl-CoA decarboxylase, a potentially toxic metabolite, to form butyryl-CoA, suggesting it might be involved in metabolite proofreading. Also has methylmalonyl-CoA decarboxylase activity at lower level.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_025855" in other vectors (4)
SKU Description Size Price
MC202725 Echdc1 (untagged) - Mouse enoyl Coenzyme A hydratase domain containing 1 (Echdc1), transcript variant 1, (10ug) 10 ug
$300.00
MG204651 Echdc1 (tGFP-tagged) - Mouse enoyl Coenzyme A hydratase domain containing 1 (Echdc1) 10 ug
$500.00
MR204651L3 Lenti ORF clone of Echdc1 (Myc-DDK-tagged) - Mouse enoyl Coenzyme A hydratase domain containing 1 (Echdc1), transcript variant 1 10 ug
$600.00
MR204651L4 Lenti ORF clone of Echdc1 (mGFP-tagged) - Mouse enoyl Coenzyme A hydratase domain containing 1 (Echdc1), transcript variant 1 10 ug
$600.00

Plasmid Map for MR204651

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Cloning scheme for MR204651

Circular map for MR204651

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