Sfxn1 (NM_027324) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR204649
Sfxn1 (Myc-DDK-tagged) - Mouse sideroflexin 1 (Sfxn1)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Sfxn1
Synonyms 2810002O05Rik; A930015P12Rik; f
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR204649 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTGGGGAAGTGCCACCCAACATTAACATCAAGGAGCCTCGATGGGACCAGAGCACGTTCATTGGCC
GAGCCAGTCACTTCTTCACGGTTACTGATCCCAGAAATATCCTTTTAACGAACGAACAGCTAGAGAATGC
GAGGAAAGTGGTGCATGACTACAGGCAAGGAATCGTTCCTGCCGGCCTCACGGAAAATGAGCTATGGAGA
GCGAAGTACGCGTACGACTCGGCCTTCCATCCTGACACCGGTGAAAAGATGACTCTGATAGGAAGAATGT
CAGCTCAGGTCCCGATGAACATGACCATCACAGGCTGCATGATGACCTTCTACCGGACCACACCGGCTGT
GCTTTTCTGGCAGTGGATAAACCAGTCCTTCAATGCCGTGGTCAACTACACCAACAGAAGCGGTGACGCT
CCCCTCACTGTGAATGAGCTGGGAACGGCTTATGTTTCTGCAACCACTGGCGCTGTGGCAACGGCCCTAG
GACTCAATGCCTTAACCAAGCGTGTCTCCCCACTGATAGGACGTTTTGTTCCCTTCGCTGCTGTAGCTGC
TGCTAACTGCATTAACATCCCACTGATGAGACAAAGGGAGCTGAAGGTTGGTATTCCTGTCACTGATGAA
AATGGCACTCGCTTGGGAGAATCGACCAACGCAGCAAAGCAAGCCATCACGCAGGTGGTCATCTCCAGGA
TCCTCATGGCAGCCCCGGGCATGGCCATCCCTCCGTTTATCATGAACACCTTGGAAAAGAAAGCCTTTTT
AAAGAGGTTCCCGTGGATGAGTGCGCCAATCCAAGTCACGTTGGTTGGCTTTTGTTTGGTATTCGCCACC
CCCCTGTGCTGTGCTCTGTTTCCTCAGAAGAGTTCCATGTCTGTGACAAGCTTGGAGGATGAGTTGCAAG
CCAGCATCCAAAGGACTCATCCTGAAATACGGCGTGTGTACTTTAACAAGGGCCTG


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR204649 protein sequence
Red=Cloning site Green=Tags(s)

MSGEVPPNINIKEPRWDQSTFIGRASHFFTVTDPRNILLTNEQLENARKVVHDYRQGIVPAGLTENELWR
AKYAYDSAFHPDTGEKMTLIGRMSAQVPMNMTITGCMMTFYRTTPAVLFWQWINQSFNAVVNYTNRSGDA
PLTVNELGTAYVSATTGAVATALGLNALTKRVSPLIGRFVPFAAVAAANCINIPLMRQRELKVGIPVTDE
NGTRLGESTNAAKQAITQVVISRILMAAPGMAIPPFIMNTLEKKAFLKRFPWMSAPIQVTLVGFCLVFAT
PLCCALFPQKSSMSVTSLEDELQASIQRTHPEIRRVYFNKGL

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_027324
ORF Size 966 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027324.5
RefSeq Size 2811 bp
RefSeq ORF 969 bp
Locus ID 14057
UniProt ID Q99JR1
Cytogenetics 13 28.4 cM
MW 35.6 kDa
Summary Mitochondrial serine transporter that mediates transport of serine into mitochondria, an important step of the one-carbon metabolism pathway (By similarity). Mitochondrial serine is converted to glycine and formate, which then exits to the cytosol where it is used to generate the charged folates that serve as one-carbon donors (By similarity). Transports both D-serine and L-serine (By similarity). Also able to transport other amino-acids, such as alanine (By similarity). May be indirectly involved in the transport of a component required for iron utilization into or out of the mitochondria (PubMed:11274051).UniProtKB/Swiss-Prot Function
Reviews
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_027324" in other vectors (4)
SKU Description Size Price
MC200419 Sfxn1 (untagged) - Mouse sideroflexin 1 (Sfxn1), (10ug) 10 ug
$300.00
MG204649 Sfxn1 (tGFP-tagged) - Mouse sideroflexin 1 (Sfxn1) 10 ug
$500.00
MR204649L3 Lenti ORF clone of Sfxn1 (Myc-DDK-tagged) - Mouse sideroflexin 1 (Sfxn1) 10 ug
$600.00
MR204649L4 Lenti ORF clone of Sfxn1 (mGFP-tagged) - Mouse sideroflexin 1 (Sfxn1) 10 ug
$600.00

Plasmid Map for MR204649

Download sequence for

Cloning scheme for MR204649

Circular map for MR204649

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products