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Sfxn3 (NM_053197) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR204636
Sfxn3 (Myc-DDK-tagged) - Mouse sideroflexin 3 (Sfxn3), transcript variant 1
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Sfxn3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR204636 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGTGACCTGCCCTTAAATATCAACATCCAGGAACCTCGGTGGGACCAAAGCACATTTCTAGGCAGAG
CCCGGCATTTCTTCACAGTCACTGATCCCCGAAATCTGCTGCTGTCCGGGGAACAGCTGGAAGCTTCCCG
GAACATCGTGCAGAATTACAGGGCTGGTGTGGCAACCCCGGGTCTCACTGAGGACCAGCTATGGCGAGCC
AAATACGTGTATGACTCAGCATTCCATCCGGACACGGGGGAGAAGGTGGTCTTGATTGGCCGTATGTCAG
CCCAGGTGCCCATGAACATGACCATTACTGGCTGCATGCTCACCTTCTACAGGAAGACTCCGACTGTGGT
GTTCTGGCAGTGGGTCAATCAGTCCTTCAATGCTATTGTGAATTACTCTAATCGCAGCGGCGATGCTCCC
ATCACTGTGCAGCAGTTGGGGACAGCCTATGTGAGTGCCACCACTGGGGCTGTGGCTACTGCTCTGGGAC
TCAAGTCTCTCACCAAGCACCTGCCCCCGCTAGTCGGTCGATTCGTGCCCTTTGCAGCTGTGGCCGCTGC
CAACTGCATCAACATCCCCCTGATGAGGCAGAGGGAGCTGCAGGTGGGCATCCCAGTGACTGATGAGGCT
GGTCAGAGGCTTGGCCACTCGGTGACTGCTGCCAAACAGGGAATCTTCCAGGTGGTGATATCAAGAATCG
GAATGGCGATTCCCGCCATGGCCATTCCCCCGGTGATCATGAACACTCTGGAGAAGAAAGACTTCCTGAA
GCGCCGTCCCTGGCTGGGGGCGCCCCTGCAGGTGGGACTGGTAGGCTTCTGCTTGGTATTTGCCACACCC
CTGTGCTGCGCTCTGTTCCCTCAGAGAAGCTCCATACATGTGACCAGGCTGGAGCCGGAGCTGAGAGCTC
AGATCCAAGCACAAAACCCCAGCATCGATGTGGTTTACTACAACAAGGGGCTT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR204636 protein sequence
Red=Cloning site Green=Tags(s)

MGDLPLNINIQEPRWDQSTFLGRARHFFTVTDPRNLLLSGEQLEASRNIVQNYRAGVATPGLTEDQLWRA
KYVYDSAFHPDTGEKVVLIGRMSAQVPMNMTITGCMLTFYRKTPTVVFWQWVNQSFNAIVNYSNRSGDAP
ITVQQLGTAYVSATTGAVATALGLKSLTKHLPPLVGRFVPFAAVAAANCINIPLMRQRELQVGIPVTDEA
GQRLGHSVTAAKQGIFQVVISRIGMAIPAMAIPPVIMNTLEKKDFLKRRPWLGAPLQVGLVGFCLVFATP
LCCALFPQRSSIHVTRLEPELRAQIQAQNPSIDVVYYNKGL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_053197
ORF Size 963 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_053197.4
RefSeq Size 2974 bp
RefSeq ORF 966 bp
Locus ID 94280
UniProt ID Q91V61
Cytogenetics 19 C3
MW 35.4 kDa
Summary Mitochondrial serine transporter that mediates transport of serine into mitochondria, an important step of the one-carbon metabolism pathway. Mitochondrial serine is converted to glycine and formate, which then exits to the cytosol where it is used to generate the charged folates that serve as one-carbon donors.UniProtKB/Swiss-Prot Function
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"NM_053197" in other vectors (4)
SKU Description Size Price
MC203802 Sfxn3 (untagged) - Mouse sideroflexin 3 (Sfxn3), transcript variant 1, (10ug) 10 ug
$300.00
MG204636 Sfxn3 (tGFP-tagged) - Mouse sideroflexin 3 (Sfxn3) 10 ug
$500.00
MR204636L3 Lenti ORF clone of Sfxn3 (Myc-DDK-tagged) - Mouse sideroflexin 3 (Sfxn3), transcript variant 1 10 ug
$600.00
MR204636L4 Lenti ORF clone of Sfxn3 (mGFP-tagged) - Mouse sideroflexin 3 (Sfxn3), transcript variant 1 10 ug
$600.00

Plasmid Map for MR204636

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR204636
Circular map for MR204636

file-alt Citations

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