Nubp1 (BC055436) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR204626
Nubp1 (Myc-DDK-tagged) - Mouse nucleotide binding protein 1 (cDNA clone MGC:65267 IMAGE:3660324)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Nubp1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR204626 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGAGGCGCCCCACGGCTGCCCCGGGGCCGACAGCGCCCAGGCCGGCCGAGGAGCCTCGTGCCAGG
GATGCCCCAACCAGAGGCTGTGCGCATCCGGAGCCGGCGCTGCTCCGGATCCCGCTGTGGAGGAAATCAG
AGAGAAGATGAAGACGGTGAGACACAAGCTCTTGGTGCTGTCTGGAAAAGGCGGCGTTGGGAAAAGCACG
TTCAGTGCACACCTCGCCCACGGCCTGGCGGAGGATGGAGACACACAGGTCGCTCTTCTGGACATCGATA
TCTGTGGGCCATCGATTCCCAAGATCATGGGCTTAGAAGGAGAGCAGGTTCACCAGAGCGGCTCCGGCTG
GTCTCCAGTGTACGTGGATGACAACCTGGGGGTGATGTCGGTGGGCTTTCTGCTTAGCAGCCCAGATGAT
GCTGTCATCTGGAGGGGACCAAAGAAAAATGGTATGATCAAGCAGTTCCTCCGTGATGTAGACTGGGGAG
ACGTGGACTACCTCATCATAGACACCCCGCCTGGCACGTCTGATGAACACCTCTCAGTGGTCCAGTACCT
GGCTGCGGCACACATTGATGGGGCAGTGATCCTCACCACCCCTCAGGAGGTGGCCCTCCAGGATGTCCGG
AAAGAGATCAGCTTCTGCCACAAGGTGAAGCTGCCCATTATTGGTGTGGTAGAGAACATGAGCGGTTTCA
TCTGCCCCAAGTGCAAGAAAGAGTCTCAGATCTTCCCTCCCACGACTGGGGGTGCAGAGGCCATGTGCCA
GGATCTGAAGATTCCACTTCTGGGCAAAGTGCCGCTGGATCCACACATAGGCAAGAGCTGTGACAAAGGC
CAGTCTTTTTTTGTCGAAGCTCCAGATTCACCAGCCACAGCAGCCTACAGAAGTATAATTCAGAGGATCC
GAGACTTTTGTAATTCTCGTCAGTCACATGCTGAGACCCTCATCAGTCCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR204626 protein sequence
Red=Cloning site Green=Tags(s)

MEEAPHGCPGADSAQAGRGASCQGCPNQRLCASGAGAAPDPAVEEIREKMKTVRHKLLVLSGKGGVGKST
FSAHLAHGLAEDGDTQVALLDIDICGPSIPKIMGLEGEQVHQSGSGWSPVYVDDNLGVMSVGFLLSSPDD
AVIWRGPKKNGMIKQFLRDVDWGDVDYLIIDTPPGTSDEHLSVVQYLAAAHIDGAVILTTPQEVALQDVR
KEISFCHKVKLPIIGVVENMSGFICPKCKKESQIFPPTTGGAEAMCQDLKIPLLGKVPLDPHIGKSCDKG
QSFFVEAPDSPATAAYRSIIQRIRDFCNSRQSHAETLISP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC055436
ORF Size 960 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC055436, AAH55436
RefSeq Size 1264 bp
RefSeq ORF 962 bp
Locus ID 26425
Cytogenetics 16 5.61 cM
MW 34.1 kDa
Summary Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The NUBP1-NUBP2 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins (By similarity). Implicated in the regulation of centrosome duplication (PubMed:16638812, PubMed:23807208). Negatively regulates cilium formation and structure (PubMed:23807208).UniProtKB/Swiss-Prot Function
Reviews
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"BC055436" in other vectors (3)
SKU Description Size Price
MG204626 Nubp1 (tGFP-tagged) - Mouse nucleotide binding protein 1 (cDNA clone MGC:65267 IMAGE:3660324) 10 ug
$500.00
MR204626L3 Lenti ORF clone of Nubp1 (Myc-DDK-tagged) - Mouse nucleotide binding protein 1 (cDNA clone MGC:65267 IMAGE:3660324) 10 ug
$600.00
MR204626L4 Lenti ORF clone of Nubp1 (mGFP-tagged) - Mouse nucleotide binding protein 1 (cDNA clone MGC:65267 IMAGE:3660324) 10 ug
$600.00

Plasmid Map for MR204626

Download sequence for

Cloning scheme for MR204626

Circular map for MR204626

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products