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Gtf2b (NM_145546) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR204535
Gtf2b (Myc-DDK-tagged) - Mouse general transcription factor IIB (Gtf2b)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gtf2b
Synonyms MGC6859
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR204535 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGTCGACCAGCCGTTTGGATGCTCTCCCAAGAGTCACATGTCCGAATCATCCAGATGCAATTTTAG
TTGAAGACTACAGAGCTGGCGATATGATCTGTCCCGAATGTGGCCTAGTTGTAGGGGACCGAGTTATTGA
TGTGGGATCTGAATGGAGAACTTTCAGCAATGACAAAGCAACAAAAGACCCATCTAGAGTTGGAGACTCT
CAGAATCCTCTTCTGAGTGATGGAGATTTGTCCACCATGATTGGCAAGGGTACAGGAGCTGCAAGTTTTG
ATGAGTTTGGCAATTCTAAGTATCAGAACCGGAGAACAATGAGTAGCTCTGATCGAGCAATGATGAACGC
ATTCAAGGAAATCACGACCATGGCGGACAGAATCAACCTCCCTCGCAATATAGTTGATCGAACAAATAAT
TTATTCAAGCAAGTGTATGAGCAGAAGAGCCTGAAGGGCAGAGCTAATGACGCGATAGCCTCTGCTTGTC
TGTACATCGCCTGTAGACAAGAAGGCGTTCCCAGGACATTTAAAGAAATATGTGCTGTGTCTCGAATCTC
TAAGAAAGAAATTGGCCGCTGTTTTAAACTGATTTTGAAAGCTCTGGAAACCAGCGTGGATCTGATCACA
ACTGGGGACTTCATGTCCAGGTTCTGCTCCAACCTTTGCCTCCCCAAGCAAGTGCAGATGGCAGCTACAC
ACATAGCCCGCAAGGCAGTGGAGCTGGACTTGGTTCCTGGCAGGAGCCCGATCTCTGTGGCGGCAGCAGC
TATTTACATGGCTTCCCAGGCTTCAGCTGAGAAGCGAACACAGAAAGAAATCGGGGATATTGCTGGTGTT
GCTGATGTTACAATCCGACAGTCCTACAGACTGATCTACCCTCGGGCTCCGGATCTCTTCCCTTCAGACT
TCAAGTTTGACACCCCAGTGGACAAATTACCCCAGCTA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR204535 protein sequence
Red=Cloning site Green=Tags(s)

MASTSRLDALPRVTCPNHPDAILVEDYRAGDMICPECGLVVGDRVIDVGSEWRTFSNDKATKDPSRVGDS
QNPLLSDGDLSTMIGKGTGAASFDEFGNSKYQNRRTMSSSDRAMMNAFKEITTMADRINLPRNIVDRTNN
LFKQVYEQKSLKGRANDAIASACLYIACRQEGVPRTFKEICAVSRISKKEIGRCFKLILKALETSVDLIT
TGDFMSRFCSNLCLPKQVQMAATHIARKAVELDLVPGRSPISVAAAAIYMASQASAEKRTQKEIGDIAGV
ADVTIRQSYRLIYPRAPDLFPSDFKFDTPVDKLPQL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_145546
ORF Size 948 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_145546.1, NP_663521.1
RefSeq Size 1267 bp
RefSeq ORF 951 bp
Locus ID 229906
UniProt ID P62915
Cytogenetics 3 H1
MW 34.8 kDa
Summary General transcription factor that plays a role in transcription initiation by RNA polymerase II (Pol II). Involved in the pre-initiation complex (PIC) formation and Pol II recruitment at promoter DNA. Together with the TATA box-bound TBP forms the core initiation complex and provides a bridge between TBP and the Pol II-TFIIF complex. Released from the PIC early following the onset of transcription during the initiation and elongation transition and reassociates with TBP during the next transcription cycle. Associates with chromatin to core promoter-specific regions. Binds to two distinct DNA core promoter consensus sequence elements in a TBP-independent manner; these IIB-recognition elements (BREs) are localized immediately upstream (BREu), 5'-GCGCGACGCC-3', and downstream (BREd), 5'-GATTGATGGTTGTG-3', of the TATA box element. Modulates transcription start site selection. Exhibits also autoacetyltransferase activity that contributes to the activated transcription.UniProtKB/Swiss-Prot Function
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"NM_145546" in other vectors (4)
SKU Description Size Price
MC203695 Gtf2b (untagged) - Mouse general transcription factor IIB (Gtf2b), (10ug) 10 ug
$300.00
MG204535 Gtf2b (tGFP-tagged) - Mouse general transcription factor IIB (Gtf2b) 10 ug
$500.00
MR204535L3 Lenti ORF clone of Gtf2b (Myc-DDK-tagged) - Mouse general transcription factor IIB (Gtf2b) 10 ug
$600.00
MR204535L4 Lenti ORF clone of Gtf2b (mGFP-tagged) - Mouse general transcription factor IIB (Gtf2b) 10 ug
$600.00

Plasmid Map for MR204535

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR204535
Circular map for MR204535

file-alt Citations

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