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Aagab (NM_025857) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR204528
Aagab (Myc-DDK-tagged) - Mouse alpha- and gamma-adaptin binding protein (Aagab)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Aagab or NM_025857"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Aagab
Synonyms 2310007F21Rik; p34
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR204528 representing NM_025857
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGCCGGTGTACCCTGCGCGCTGGTTACCAGCTGCTCCGCCACCTTCACCGGGGACCGGCTGGTGC
AACATATCCTTGGGACAGAAGATGCTGTTGTGGAAGCAACTTCCAGCGATGCTGTGAGGTTTTACCCCTG
GACCATCGATAACAAGTACTATTCCGCAGAGATCAACCTGTGCGTGGTGCCAAGCAAGTTCCTGGTCACT
GCTGAGATTGCCGAGTCAGTCCAGGCGTTTGTAGTTTACTTTGACAGCACACAGAAATCAGGTCTTGACA
GCGTCTCCTCGTGGCTCCCTCTGGCAGAAGCATGGCTAGCTGAGGTGATGATCCTGGTCTGTGATAGAGT
GTGTGACGATGGAATAAACCGACAACAGGCTCAAGAGTGGTGCATCAAACATGGCTTTGAGTTGGTGGAA
CTCAACCCAGAGGAGCTGCCCGAGGAGGACGATGACTTCCCTGAATCTACAGGTGTAAAGCGGATTGTAC
AAGCATTGAATGCCAATGTCTGGTCCAATGTCGTGATGAAGAGTGACAGGAGCCAGGGCTTCAGCCTTCT
CAACTCACTGGCTGGGGCAAACCGCAGAGTTGCATCAGCAGAGAGCTGTCACTCTGAGCAGCAGGAGCCG
TCGCCAACAGCTGAGAGGACAGAGTCCCTCCCTGGCCATCACAGTGGTGCGTGTGGCTCAGCAGGCGCAC
AGGTTGACAGCATTGTCGACCCCATGTTAGACCTGGATATTCAAGAACTAGCCAGTCTTACCACTGGAGG
AGGTGATCTGGAGAACTTCGAGAGACTCTTTTCAAAGTTAAAAGAAATGAAAGACAAGGCTGCGACGCTT
CCTCATGAGCAAAGAAAGTTGCATGCAGAAAAGGTGGCCAAAGCCTTCTGGATGGCAATAGGAGGGGACC
GAGATGAGATCGAGGGCCTTTCTTCTGATGACGAGCAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR204528 representing NM_025857
Red=Cloning site Green=Tags(s)

MAAGVPCALVTSCSATFTGDRLVQHILGTEDAVVEATSSDAVRFYPWTIDNKYYSAEINLCVVPSKFLVT
AEIAESVQAFVVYFDSTQKSGLDSVSSWLPLAEAWLAEVMILVCDRVCDDGINRQQAQEWCIKHGFELVE
LNPEELPEEDDDFPESTGVKRIVQALNANVWSNVVMKSDRSQGFSLLNSLAGANRRVASAESCHSEQQEP
SPTAERTESLPGHHSGACGSAGAQVDSIVDPMLDLDIQELASLTTGGGDLENFERLFSKLKEMKDKAATL
PHEQRKLHAEKVAKAFWMAIGGDRDEIEGLSSDDEH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025857
ORF Size 948 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025857.3
RefSeq Size 2600 bp
RefSeq ORF 951 bp
Locus ID 66939
UniProt ID Q8R2R3
Cytogenetics 9 C
MW 34.5 kDa
Summary May be involved in endocytic recycling of growth factor receptors such as EGFR.UniProtKB/Swiss-Prot Function
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"NM_025857" in other vectors (3)
SKU Description Size Price
MG204528 Aagab (tGFP-tagged) - Mouse RIKEN cDNA 2310007F21 gene (2310007F21Rik) 10 ug
$500.00
MR204528L3 Lenti ORF clone of Aagab (Myc-DDK-tagged) - Mouse alpha- and gamma-adaptin binding protein (Aagab) 10 ug
$600.00
MR204528L4 Lenti ORF clone of Aagab (mGFP-tagged) - Mouse alpha- and gamma-adaptin binding protein (Aagab) 10 ug
$600.00

Plasmid Map for MR204528

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR204528
Circular map for MR204528

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