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Slc9a3r2 (BC070458) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR204526
Slc9a3r2 (Myc-DDK-tagged) - Mouse solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 2 (cDNA clone MGC:96425 IMAGE:30603578)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Slc9a3r2
Synonyms E3karp, Sip-1, Tka-1, Octs2, NHERF-2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR204526 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGCGCTGGAATCGCTGCGGCCCCGCTTGTGTCGCCTGGTGCGCGGAGAGCAAGGGTACGGCTTTC
ACCTGCATGGCGAGAAAGGCCGCCGTGGCCAGTTCATCCGGCGCGTGGAGCCAGGCTCCCCAGCCGAGGC
GGCCGCGCTGCGCGCCGGGGACCGCCTGGTTGAGGTCAACGGCGTCAACGTAGAGGGCGAGACGCATCAC
CAGGTGGTACAGAGGATCAAGGCTGTGGAGGGGCAGACCCAGCTGCTTGTGGTGGACAAGGAGACCGATG
AGGAGCTCTGCCGGCGGCAACTGACCTGCACTGAGGAGATGGCCCATCGAGGGCTTCCCCCAGCCCACAA
CCCCTGGGAGCCAAAGCCGGACTGGGCATGCTCGGGCAGCCTTGGCTCGGACACTGGCCAGAAGGATGTC
AATGGGCCCCCAAGAGAGCTGCGTCCCCGGCTCTGCCACTTGCGAAGGGGACCCCAGGGCTACGGGTTCA
ACCTGCATAGTGACAAGTCCCGGCCTGGCCAGTACATCCGCTCTGTGGACCCTGGCTCTCCTGCTTCTCA
CTCTGGCCTCCGTGCCCAGGATCGGCTCATTGAGGTGAATGGGCAGAACGTGGAGGGGCTGCGACATGCC
GAGGTTGTCGCCAGAATCAAGGCACAGGAGGACGAGGCCCGGCTGCTGGTAGTCGATCCTGAGACTGATG
AACACTTCAAGCGGTTGAGGGTCATACCCACTGAGGAACATGTAGAAGGTCCACTGCCATCACCAGTCAC
GAACGGGACCAGTCCTGCCCAGGATGGCAGTACCTGGAAGCGAGACCCCTTCCAGGAAAGTGGCCTCCAC
CTGAGTCCCACAGCAGCTGAGGCCAAGGAGAAGGCTCGAGCTACTCGGGTCAACAAGCGGGCACCACAGA
TGGACTGGAACCGGAAGCGAGAGATCTTCAGCAACTTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR204526 protein sequence
Red=Cloning site Green=Tags(s)

MAALESLRPRLCRLVRGEQGYGFHLHGEKGRRGQFIRRVEPGSPAEAAALRAGDRLVEVNGVNVEGETHH
QVVQRIKAVEGQTQLLVVDKETDEELCRRQLTCTEEMAHRGLPPAHNPWEPKPDWACSGSLGSDTGQKDV
NGPPRELRPRLCHLRRGPQGYGFNLHSDKSRPGQYIRSVDPGSPASHSGLRAQDRLIEVNGQNVEGLRHA
EVVARIKAQEDEARLLVVDPETDEHFKRLRVIPTEEHVEGPLPSPVTNGTSPAQDGSTWKRDPFQESGLH
LSPTAAEAKEKARATRVNKRAPQMDWNRKREIFSNF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC070458
ORF Size 948 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC070458, AAH70458
RefSeq Size 2052 bp
RefSeq ORF 950 bp
Locus ID 65962
Cytogenetics 17 A3.3
MW 35.3 kDa
Summary Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May also act as scaffold protein in the nucleus (By similarity).UniProtKB/Swiss-Prot Function
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"BC070458" in other vectors (4)
SKU Description Size Price
MC217816 Slc9a3r2 (untagged) - Mouse solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 2 (cDNA clone MGC:96425 IMAGE:30603578),, (10ug) 10 ug
$330.00
MG204526 Slc9a3r2 (tGFP-tagged) - Mouse solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 2 (cDNA clone MGC:96425 IMAGE:30603578) 10 ug
$500.00
MR204526L3 Lenti ORF clone of Slc9a3r2 (Myc-DDK-tagged) - Mouse solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 2 (cDNA clone MGC:96425 IMAGE:30603578) 10 ug
$600.00
MR204526L4 Lenti ORF clone of Slc9a3r2 (mGFP-tagged) - Mouse solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 2 (cDNA clone MGC:96425 IMAGE:30603578) 10 ug
$600.00

Plasmid Map for MR204526

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR204526
Circular map for MR204526

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