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Pitx1 (NM_011097) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR204502
Pitx1 (Myc-DDK-tagged) - Mouse paired-like homeodomain transcription factor 1 (Pitx1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pitx1
Synonyms Bft; P-OTX; Potx; Ptx1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR204502 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACGCCTTCAAGGGAGGCATGAGCCTGGAGAGGCTACCGGAGGGGCTGCGGCCACCGCCACCACCAC
CGCACGACATGGGGCCCAGCTTCCACCTGGCCCGCGCCGCCGACCCCCGGGAGCCGCTGGAGAACTCCGC
CAGCGAATCGTCCGACGCTGATCTGCCAGACAAGGAGCGCGGTGGGGAAGCCAAGGGGCCAGAGGATGGT
GGCGCGGGCAGTGCTGGCTGCGGCGGCGGTGCAGAGGACCCAGCTAAGAAGAAGAAACAGCGGCGGCAAC
GCACTCACTTCACAAGCCAGCAGTTGCAAGAGCTGGAGGCCACGTTCCAAAGGAACCGCTACCCCGACAT
GAGCATGAGAGAGGAGATCGCGGTGTGGACCAACCTCACTGAACCGCGAGTGCGGGTCTGGTTCAAGAAC
CGGCGAGCCAAATGGCGCAAGCGGGAGCGGAACCAGCAGTTGGACCTGTGCAAGGGCGGCTATGTGCCGC
AGTTCAGCGGCCTGGTGCAGCCCTACGAGGACGTGTACGCTGCCGGCTACTCCTACAACAACTGGGCGGC
CAAGAGCCTGGCCCCGGCGCCGCTGTCTACCAAGAGCTTTACCTTCTTCAACTCCATGAGCCCGCTCTCC
TCTCAATCCATGTTCTCGGCCCCCAGCTCCATCTCTTCCATGACCATGCCGTCCAGCATGGGCCCGGGCG
CCGTGCCCGGCATGCCCAACTCGGGCCTCAACAACATCAACAACCTCACCGGCTCCTCGCTCAACTCGGC
CATGTCGCCGGGCGCCTGCCCCTACGGCACCCCAGCCTCGCCCTACAGCGTCTACCGGGACACGTGCAAC
TCGAGCCTTGCCAGCCTGCGGCTCAAGTCCAAGCAGCACTCGTCGTTTGGCTATGGCGGTCTGCAGGGTC
CGGCGTCGGGGCTCAACGCTTGCCAGTACAACAGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR204502 protein sequence
Red=Cloning site Green=Tags(s)

MDAFKGGMSLERLPEGLRPPPPPPHDMGPSFHLARAADPREPLENSASESSDADLPDKERGGEAKGPEDG
GAGSAGCGGGAEDPAKKKKQRRQRTHFTSQQLQELEATFQRNRYPDMSMREEIAVWTNLTEPRVRVWFKN
RRAKWRKRERNQQLDLCKGGYVPQFSGLVQPYEDVYAAGYSYNNWAAKSLAPAPLSTKSFTFFNSMSPLS
SQSMFSAPSSISSMTMPSSMGPGAVPGMPNSGLNNINNLTGSSLNSAMSPGACPYGTPASPYSVYRDTCN
SSLASLRLKSKQHSSFGYGGLQGPASGLNACQYNS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_011097
ORF Size 945 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_011097.2, NP_035227.1
RefSeq Size 2448 bp
RefSeq ORF 948 bp
Locus ID 18740
UniProt ID P70314
Cytogenetics 13 30.06 cM
MW 34.1 kDa
Summary Sequence-specific transcription factor that binds gene promoters and activates their transcription. May play a role in the development of anterior structures, and in particular, the brain and facies and in specifying the identity or structure of hindlimb. Can independently activate and synergize with PIT-1 on pituitary-specific target gene promoters, thus may subserve functions in generating both precursor and specific cell phenotypes in the anterior pituitary gland and in several other organs. Can activate pituitary transcription of the proopiomelanocortin gene.UniProtKB/Swiss-Prot Function
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Documents
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"NM_011097" in other vectors (4)
SKU Description Size Price
MC200808 Pitx1 (untagged) - Mouse paired-like homeodomain transcription factor 1 (Pitx1), (10ug) 10 ug
$300.00
MG204502 Pitx1 (tGFP-tagged) - Mouse paired-like homeodomain transcription factor 1 (Pitx1) 10 ug
$500.00
MR204502L3 Lenti ORF clone of Pitx1 (Myc-DDK-tagged) - Mouse paired-like homeodomain transcription factor 1 (Pitx1) 10 ug
$600.00
MR204502L4 Lenti ORF clone of Pitx1 (mGFP-tagged) - Mouse paired-like homeodomain transcription factor 1 (Pitx1) 10 ug
$600.00

Plasmid Map for MR204502

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR204502
Circular map for MR204502

file-alt Citations

The Polycomb-Dependent Epigenome Controls β Cell Dysfunction, Dedifferentiation, and Diabetes
Lu, Heyne, Dror et al
Cell Metab. (2018) 27 (6), 1294-1308.e7 DOI: 10.1016/j.cmet.2018.04.013
AI Summary: OriGene’s mouse cDNA ORF clones for six different transcription factors were used to over-express these factors in Min6-B1 cells, enabling the study of their roles in β cell identity and dysfunction in the context of diabetes.
Snippet: Transcription Factor Over-Expression Min6-B1 cell line were electroporated using Nucleofector II device (Lonza, Kit T, Program O-017) with pMAXGFP (Lonza) or mouse cDNA ORF clones of the 6 different transcription factors (Origene). 4 days after electroporation, cells were harvested, trypsinized and single cells were sorted in 384-well plates containing 240 nL of primer mix and 1.2 μL of PCR encaps...
FigureFigureFigure
Showing 1-1 of 1 papers.
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