Hmox2 (NM_010443) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR204499
Hmox2 (Myc-DDK-tagged) - Mouse heme oxygenase (decycling) 2 (Hmox2), transcript variant 2
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Hmox2
Synonyms HO-2; HO2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR204499 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTTCAGAGGTGGAGACCTCGGAGGGGGTAGATGAGTCAGAGAAGAACTCTATGGCACCAGAAAAGG
AAAACCATACCAAAATGGCAGACCTTTCTGAGCTCCTGAAGGAAGGGACCAAGGAAGCACATGACCGAGC
AGAAAATACCCAGTTTGTCAAAGACTTCTTGAAAGGAAACATTAAGAAGGAGCTATTTAAGCTGGCCACC
ACTGCACTTTACTTCACATACTCAGCCCTTGAGGAGGAAATGGACCGCAACAAGGACCACCCAGCCTTCG
CCCCCTTATATTTCCCCACGGAGCTACACCGGAAGGCAGCACTGATCAAGGACATGAAGTATTTCTTTGG
TGAAAACTGGGAGGAGCAGGTGAAGTGCTCTGAGGCTGCCCAGAAGTATGTGGATCGGATTCACTATGTA
GGGCAAAATGAGCCAGAGCTGCTGGTGGCCCATGCTTATACTCGTTACATGGGGGACCTTTCAGGAGGCC
AGGTACTGAAGAAGGTTGCCCAGAGGGCACTAAAACTTCCCAGCACTGGGGAAGGGACCCAATTCTACCT
GTTTGAGCATGTGGACAATGCCCAGCAATTCAAGCAGTTCTACCGCGCTAGAATGAATGCCTTGGACCTG
AATTTGAAGACCAAAGAGAGGATTGTGGAGGAGGCCAATAAAGCCTTTGAATATAACATGCAGATATTCA
GTGAACTGGACCAGGCTGGCTCCATGCTAGCAAGAGAAACCCTGGAGGATGGGCTCCCGGTACATGATGG
GAAGGGAGATATACGTAAATGCCCCTTTTATGCTGCTCAGCCAGACAAAGGTACACTAGGAGGCAGCAAC
TGCCCCTTCCAGACAACCGTGGCTGTGCTGAGGAAGCCTAGCCTGCAGCTCATTCTGGCTGCCAGTGTGG
CCTTGGTAGCTGGACTCTTGGCCTGGTACTACATG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR204499 protein sequence
Red=Cloning site Green=Tags(s)

MSSEVETSEGVDESEKNSMAPEKENHTKMADLSELLKEGTKEAHDRAENTQFVKDFLKGNIKKELFKLAT
TALYFTYSALEEEMDRNKDHPAFAPLYFPTELHRKAALIKDMKYFFGENWEEQVKCSEAAQKYVDRIHYV
GQNEPELLVAHAYTRYMGDLSGGQVLKKVAQRALKLPSTGEGTQFYLFEHVDNAQQFKQFYRARMNALDL
NLKTKERIVEEANKAFEYNMQIFSELDQAGSMLARETLEDGLPVHDGKGDIRKCPFYAAQPDKGTLGGSN
CPFQTTVAVLRKPSLQLILAASVALVAGLLAWYYM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_010443
ORF Size 945 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_010443.2, NP_034573.2
RefSeq Size 1274 bp
RefSeq ORF 948 bp
Locus ID 15369
UniProt ID O70252
Cytogenetics 16 2.46 cM
MW 35.7 kDa
Summary Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Heme oxygenase 2 could be implicated in the production of carbon monoxide in brain where it could act as a neurotransmitter.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_010443" in other vectors (4)
SKU Description Size Price
MC207302 Hmox2 (untagged) - Mouse heme oxygenase (decycling) 2 (Hmox2), transcript variant 2, (10ug) 10 ug
$300.00
MG204499 Hmox2 (tGFP-tagged) - Mouse heme oxygenase (decycling) 2 (Hmox2) 10 ug
$500.00
MR204499L3 Lenti ORF clone of Hmox2 (Myc-DDK-tagged) - Mouse heme oxygenase (decycling) 2 (Hmox2), transcript variant 2 10 ug
$600.00
MR204499L4 Lenti ORF clone of Hmox2 (mGFP-tagged) - Mouse heme oxygenase (decycling) 2 (Hmox2), transcript variant 2 10 ug
$600.00

Plasmid Map for MR204499

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Cloning scheme for MR204499

Circular map for MR204499

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