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Eif2s1 (NM_026114) Mouse Tagged Lenti ORF Clone

  • LentiORF®
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SKU
MR204498L3
Lenti ORF clone of Eif2s1 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 2, subunit 1 alpha (Eif2s1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $600.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Eif2s1
Synonyms 35kDa; 0910001O23Rik; 2410026C18Rik; Eif2a; eIF2alpha
Vector pLenti-C-Myc-DDK-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(MR204498).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_026114
ORF Size 945 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026114.2
RefSeq Size 3152 bp
RefSeq ORF 948 bp
Locus ID 13665
UniProt ID Q6ZWX6
Cytogenetics 12 C3
Summary Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B (By similarity).UniProtKB/Swiss-Prot Function
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"NM_026114" in other vectors (4)
SKU Description Size Price
MC201485 Eif2s1 (untagged) - Mouse eukaryotic translation initiation factor 2, subunit 1 alpha (Eif2s1), (10ug) 10 ug
$300.00
MG204498 Eif2s1 (tGFP-tagged) - Mouse eukaryotic translation initiation factor 2, subunit 1 alpha (Eif2s1) 10 ug
$500.00
MR204498 Eif2s1 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 2, subunit 1 alpha (Eif2s1) 10 ug
$289.00 $300.00
MR204498L4 Lenti ORF clone of Eif2s1 (mGFP-tagged) - Mouse eukaryotic translation initiation factor 2, subunit 1 alpha (Eif2s1) 10 ug
$600.00

Plasmid Map for MR204498L3

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Cloning scheme for MR204498L3
Circular map for MR204498L3

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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