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Prss30 (NM_013921) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR204401
Prss30 (Myc-DDK-tagged) - Mouse protease, serine, 30 (Prss30)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Prss30
Synonyms 2010015P21Rik; Disp; Disp1; Tmprss8
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR204401 representing NM_013921
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGTCCCGGGCAAGGTGCATCTTTCTTCTTTTGCTGCAGATCCTGACAAGGGCACGTGGAGATATAT
TGCCCTCAGTATGTGGCCACTCCCGAGATGCTGGAAAGATCGTGGGTGGTCAAGATGCTTTGGAAGGACA
ATGGCCTTGGCAAGTCAGCCTGTGGATAACCGAGGATGGCCACATATGCGGGGGCTCCCTCATCCACGAG
GTGTGGGTGCTCACGGCTGCCCACTGCTTCCGCAGGTCTCTGAACCCTAGCTTCTACCATGTGAAGGTTG
GAGGGCTGACACTTTCCCTTCTGGAACCCCACTCAACTTTAGTGGCCGTGAGGAACATCTTCGTGCATCC
CACCTACCTCTGGGCAGATGCGTCTAGCGGAGACATTGCCCTGGTGCAGCTGGATACTCCTCTGCGGCCC
TCTCAGTTCACTCCTGTCTGCCTCCCAGCAGCCCAGACTCCTCTCACCCCTGGGACTGTCTGCTGGGTGA
CAGGCTGGGGAGCCACCCAGGAAAGAGACATGGCAAGTGTCCTCCAGGAGTTAGCTGTGCCTCTCTTGGA
CTCAGAGGACTGTGAAAAGATGTACCACACCCAGGGGAGCAGCCTGTCAGGGGAGCGAATCATCCAGTCG
GACATGCTCTGTGCAGGCTACGTAGAAGGCCAGAAGGACTCCTGCCAGGGTGACTCTGGAGGACCGCTCG
TTTGTTCCATCAACAGTTCTTGGACCCAGGTCGGGATCACCAGCTGGGGTATTGGCTGCGCTCGGCCATA
CAGGCCTGGTGTCTACACTCGGGTGCCCACCTATGTGGACTGGATTCAAAGGATCCTGGCAGAGAACCAC
TCTGATGCCTATGGGTACCACTCCAGTGCCTCTGCAGCATACCAAATGCTGCTGCCAGTGTTGCTGGCCG
TTGCCTTGCCAGGGTCCCTA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR204401 representing NM_013921
Red=Cloning site Green=Tags(s)

MESRARCIFLLLLQILTRARGDILPSVCGHSRDAGKIVGGQDALEGQWPWQVSLWITEDGHICGGSLIHE
VWVLTAAHCFRRSLNPSFYHVKVGGLTLSLLEPHSTLVAVRNIFVHPTYLWADASSGDIALVQLDTPLRP
SQFTPVCLPAAQTPLTPGTVCWVTGWGATQERDMASVLQELAVPLLDSEDCEKMYHTQGSSLSGERIIQS
DMLCAGYVEGQKDSCQGDSGGPLVCSINSSWTQVGITSWGIGCARPYRPGVYTRVPTYVDWIQRILAENH
SDAYGYHSSASAAYQMLLPVLLAVALPGSL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_013921
ORF Size 930 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013921.3, NP_038949.2
RefSeq Size 1469 bp
RefSeq ORF 933 bp
Locus ID 30943
UniProt ID Q9QYZ9
Cytogenetics 17 A3.3
MW 33.7 kDa
Summary Selectively cleaves synthetic peptide substrates of trypsin. Activates the epithelial sodium channel ENaC (By similarity).UniProtKB/Swiss-Prot Function
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"NM_013921" in other vectors (4)
SKU Description Size Price
MC204284 Prss30 (untagged) - Mouse protease, serine, 30 (Prss30), (10ug) 10 ug
$300.00
MG204401 Prss30 (tGFP-tagged) - Mouse transmembrane protease, serine 8 (intestinal) (Tmprss8) 10 ug
$500.00
MR204401L3 Lenti ORF clone of Prss30 (Myc-DDK-tagged) - Mouse protease, serine, 30 (Prss30) 10 ug
$600.00
MR204401L4 Lenti ORF clone of Prss30 (mGFP-tagged) - Mouse protease, serine, 30 (Prss30) 10 ug
$600.00

Plasmid Map for MR204401

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR204401
Circular map for MR204401

file-alt Citations

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