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Tpm2 (BC014809) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR204354
Tpm2 (Myc-DDK-tagged) - Mouse tropomyosin 2, beta (cDNA clone MGC:18587 IMAGE:3497670)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
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Other products for "Tpm2 or BC014809"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tpm2
Synonyms Tpm-2; Trop-2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR204354 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACGCCATCAAGAAGAAGATGCAGATGCTGAAACTGGACAAGGAGAATGCCATCGACCGCGCGGAGC
AGGCCGAAGCCGACAAAAAGCAAGCTGAAGACCGATGCAAGCAGCTGGAGGAAGAGCAGCAGGCCCTCCA
GAAGAAGCTGAAGGGGACAGAGGACGAGGTGGAAAAGTATTCCGAGTCCGTGAAGGATGCCCAGGAGAAA
CTGGAGCAGGCTGAGAAGAAGGCCACCGACGCTGAAGCAGATGTGGCCTCTCTGAACCGCCGCATTCAGC
TCGTAGAGGAGGAGTTGGATCGGGCACAGGAGCGCCTGGCTACAGCCTTGCAAAAGCTGGAGGAGGCTGA
GAAAGCCGCGGATGAGAGCGAGAGAGGAATGAAGGTCATTGAAAACCGGGCCATGAAGGATGAGGAAAAG
ATGGAGCTGCAGGAGATGCAGCTGAAGGAAGCCAAGCACATCGCTGAGGACTCAGACCGCAAATATGAGG
AGGTGGCCAGGAAGCTGGTGATCCTGGAAGGGGAGCTGGAGCGCTCGGAAGAGAGAGCCGAGGTGGCTGA
GAGCCGAGCCAGACAGCTGGAGGAGGAGCTTCGAACCATGGACCAAGCCCTCAAGTCGCTGATAGCCTCA
GAGGAGGAGGTAGTAGCCTCTCTGGGCCTTTCTAGGCAATGGCACCTTTTCTCACTCACCTCACGTCCCT
CCCCAGTGCAGCGTCTCAGGCTACTGTCTCTCTCCTGCAGCAGGAGCGGGGCGGGGGCAGGGCTTCCAAA
CTCAGCCTGCTGTCACTCTTCTCTCACCACTTTGCTCTTCTCTTTTCTCCTCCGCCCCTCCCCCACTGTG
CCACCCCATCCCCCCCCCCCCCGCCCGCCCCACTGCCTCACCTCACTGTGCCCTCACGCTCCATCCTGCC
ACACGCCCCTGCAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR204354 protein sequence
Red=Cloning site Green=Tags(s)

MDAIKKKMQMLKLDKENAIDRAEQAEADKKQAEDRCKQLEEEQQALQKKLKGTEDEVEKYSESVKDAQEK
LEQAEKKATDAEADVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIENRAMKDEEK
MELQEMQLKEAKHIAEDSDRKYEEVARKLVILEGELERSEERAEVAESRARQLEEELRTMDQALKSLIAS
EEEVVASLGLSRQWHLFSLTSRPSPVQRLRLLSLSCSRSGAGAGLPNSACCHSSLTTLLFSFLLRPSPTV
PPHPPPPRPPHCLTSLCPHAPSCHTPLQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC014809
ORF Size 924 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC014809, AAH14809
RefSeq Size 1561 bp
RefSeq ORF 926 bp
Locus ID 22004
Cytogenetics 4 A5
MW 34.8 kDa
Summary This gene belongs to the tropomyosin family which encodes proteins that bind to actin filaments and stabilize them by regulating access to actin modifying proteins. The encoded protein is a high molecular weight tropomyosin expressed in slow skeletal muscle. In humans, mutations in this gene are associated with nemaline myopathy, cap disease and distal arthrogryposis syndromes. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. provided by RefSeq, Apr 2013
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"BC014809" in other vectors (4)
SKU Description Size Price
MC200845 Tpm2 (untagged) - Mouse tropomyosin 2, beta (cDNA clone MGC:18587 IMAGE:3497670), (10ug) 10 ug
$300.00
MG204354 Tpm2 (tGFP-tagged) - Mouse tropomyosin 2, beta (cDNA clone MGC:18587 IMAGE:3497670) 10 ug
$500.00
MR204354L3 Lenti ORF clone of Tpm2 (Myc-DDK-tagged) - Mouse tropomyosin 2, beta (cDNA clone MGC:18587 IMAGE:3497670) 10 ug
$600.00
MR204354L4 Lenti ORF clone of Tpm2 (mGFP-tagged) - Mouse tropomyosin 2, beta (cDNA clone MGC:18587 IMAGE:3497670) 10 ug
$600.00

Plasmid Map for MR204354

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR204354
Circular map for MR204354

file-alt Citations

Amelioration of Congenital Tufting Enteropathy in EpCAM (TROP1)-Deficient Mice via Heterotopic Expression of TROP2 in Intestinal Epithelial Cells
Nakato, Morimura, Lu et al
Cells (2020) 9 (8) DOI: 10.3390/cells9081847
Cited by 14
AI Summary: OriGene’s pCMV6 backbone expression plasmids containing full-length murine TROP2 and human EpCAM cDNA were used to generate transgenic mice for studying the functional roles of these proteins in intestinal epithelial cells.
Snippet: Transgenic mice expressing murine TROP2 and human EpCAM in IEC were generated using a villin promotor [12] and full-length murine TROP2 and human EpCAM cDNA prepared from corresponding pCMV6 backbone expression plasmids (Origene, Rockville, MD Cached
Showing 1-1 of 1 papers.
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