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Cope (NM_021538) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR204352
Cope (Myc-DDK-tagged) - Mouse coatomer protein complex, subunit epsilon (Cope)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cope
Synonyms 1110005D17Rik; Cope1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR204352 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTCCTCCGGTTCCTGGCGCGGTCTCTGGCGGCTCCGGAGAGGTAGATGAGCTGTTCGACGTGAAGA
ACGCTTTCTACATCGGCAGCTACCAGCAGTGCATCAACGAGGCTCAGCGCGTGAAGCTCTCCAGTCCTGA
GCGGGAAGTAGAGAGGGATGTCTTCCTATACAGAGCATACCTCGCACAGAGGAAGTATGGCGTGGTCCTG
GATGAGATCAAACCCTCCTCGGCCCCAGAACTCCAGGCTGTGCGCATGTTTGCTGAGTACCTTGCCAGTG
AGAACCAGAGGGACAGCATCGTGCTGGAGCTGGATCGGGAGATGAGCAGGAGTGTGGATGTGACCAATAC
CACTTTCCTGCTCATGGCTGCCTCCATCTACTTCCACGACCAGAACCCGGATGCAGCCCTGCGAACCCTG
CACCAGGGTGACGGCCTTGAGTGCATGGCCATGACGATTCAGATCCTCCTCAAGCTGGACAGGCTGGACC
TAGCCCGGAAGGAGCTGAAGAAGATGCAGGACCAAGATGAGGACGCCACCCTTACCCAGCTAGCCACTGC
CTGGGTCAACCTGGCTGTGGGTGGTGAGAAGCTACAAGAAGCCTACTACATATTCCAAGAGCTGGCCGAC
AAGTGCTCCCCCACACTGCTGCTGCTCAATGGCCAGGCAGCCTGCCACTCGGCACAGGGCCGCTGGGAGA
CTGCAGAGGGTGTGCTGCAAGAGGCACTGGACAAGGACAGCGGCCACCCTGAGACCCTCATCAATCTCAT
TGTACTGTCACAGCACCTGGGCAAGCCCCCTGAGGTGACAAACCGATACTTGTCACAGCTGAAGGATGCA
CACAGGGCCCACCCCTTCATCAAGGAGTACCAGGCCAAGGAGAACGATTTCGATCGCCTGGCAATGCAGT
ATGCGCCCAGTGCT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR204352 protein sequence
Red=Cloning site Green=Tags(s)

MAPPVPGAVSGGSGEVDELFDVKNAFYIGSYQQCINEAQRVKLSSPEREVERDVFLYRAYLAQRKYGVVL
DEIKPSSAPELQAVRMFAEYLASENQRDSIVLELDREMSRSVDVTNTTFLLMAASIYFHDQNPDAALRTL
HQGDGLECMAMTIQILLKLDRLDLARKELKKMQDQDEDATLTQLATAWVNLAVGGEKLQEAYYIFQELAD
KCSPTLLLLNGQAACHSAQGRWETAEGVLQEALDKDSGHPETLINLIVLSQHLGKPPEVTNRYLSQLKDA
HRAHPFIKEYQAKENDFDRLAMQYAPSA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_021538
ORF Size 924 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_021538.1, NP_067513.1
RefSeq Size 1043 bp
RefSeq ORF 927 bp
Locus ID 59042
UniProt ID O89079
Cytogenetics 8 B3.3
MW 34.6 kDa
Summary The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. The coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated with ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors (By similarity).UniProtKB/Swiss-Prot Function
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"NM_021538" in other vectors (4)
SKU Description Size Price
MC203696 Cope (untagged) - Mouse coatomer protein complex, subunit epsilon (Cope), (10ug) 10 ug
$300.00
MG204352 Cope (tGFP-tagged) - Mouse coatomer protein complex, subunit epsilon (Cope) 10 ug
$500.00
MR204352L3 Lenti ORF clone of Cope (Myc-DDK-tagged) - Mouse coatomer protein complex, subunit epsilon (Cope) 10 ug
$600.00
MR204352L4 Lenti ORF clone of Cope (mGFP-tagged) - Mouse coatomer protein complex, subunit epsilon (Cope) 10 ug
$600.00

Plasmid Map for MR204352

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR204352
Circular map for MR204352

file-alt Citations

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