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Mrpl2 (NM_025302) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR204304
Mrpl2 (Myc-DDK-tagged) - Mouse mitochondrial ribosomal protein L2 (Mrpl2), nuclear gene encoding mitochondrial protein
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mrpl2
Synonyms CGI-22; L2mt; MRP-L2; MRP-L14; Rpml14
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR204304 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGCTGTGTGCGCTGACGAGTGCTCTGCGCTCTTTGAGCCTGGCATCCGCGGCCATTACCGCCCGGG
TTCCGACTCTGCTCCCTGCTGCCCAGATACAGAGCAATGTCCTCCTTCAGCTGCCCCCTGCCTTGGTGTC
GCCCTCTTACCGCCCAGTGCATATGTCTGCGGACCGCAGTGCCAAGTTTGTGTCCTGGAAGAGTCGTACC
AAGTACACAGTTAAACCAGTGAAGATGAGGAAGTCTGGGGGTCGAGACCATACAGGCCGCATCCGAGTGC
ATGGTATCGGTGGAGGCCACAAACAGAATTATCGCATGATTGACTTTCTACGGTTCCGGCCAGAAAAGGA
AAAGGCACCAGAACCGTTTGAGGAGAAGGTTGTGGTAGTCCGCTATGATCCTTGTAGGTCTGCAGACATA
GCTCTGGTTGCTGGGGGTAGCCGGAAGCGCTGGATCATTGCCACGGAAAACATGAAGGCTGGAGATACAA
TCCTGAACTCTAATCACATAGGCAGGATGGCAGTGGCCGCCCAGGAGGGGGATGCACATCCACTTGGGGC
GCTGCCTGTGGGGACCCTCATCAACAACGTAGAGAGCGAGCCTGGCCGGGGAGCCCAGTATATCCGAGCT
GCAGGGACATGCGGTGTGCTGCTTCGGAAGGTGAACGGGACAGCCATTATCCAGCTGCCCTCGAAGAGGC
AAATGCAGGTGCTGGAGTCATGCACTGCAACTGTAGGCCGAGTATCCAACGTTAACCATAACCAGCGGGT
CATCGGCAAGGCAGGAAGGAACCGCTGGCTGGGCAAGAGGCCTAACAGTGGGCTGTGGCAACGCAAGGGA
GGCTGGGCCGGCCGAAAGATTCGGCCACTACCACCCATGAAGAGTTATGTGAAGCTGCCCTCCGCTGCTG
CCCAAAAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR204304 protein sequence
Red=Cloning site Green=Tags(s)

MALCALTSALRSLSLASAAITARVPTLLPAAQIQSNVLLQLPPALVSPSYRPVHMSADRSAKFVSWKSRT
KYTVKPVKMRKSGGRDHTGRIRVHGIGGGHKQNYRMIDFLRFRPEKEKAPEPFEEKVVVVRYDPCRSADI
ALVAGGSRKRWIIATENMKAGDTILNSNHIGRMAVAAQEGDAHPLGALPVGTLINNVESEPGRGAQYIRA
AGTCGVLLRKVNGTAIIQLPSKRQMQVLESCTATVGRVSNVNHNQRVIGKAGRNRWLGKRPNSGLWQRKG
GWAGRKIRPLPPMKSYVKLPSAAAQN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025302
ORF Size 918 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025302.4
RefSeq Size 1012 bp
RefSeq ORF 921 bp
Locus ID 27398
UniProt ID Q9D773
Cytogenetics 17 C
MW 33.3 kDa
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Documents
Resources
"NM_025302" in other vectors (3)
SKU Description Size Price
MG204304 Mrpl2 (tGFP-tagged) - Mouse mitochondrial ribosomal protein L2 (Mrpl2) 10 ug
$500.00
MR204304L3 Lenti ORF clone of Mrpl2 (Myc-DDK-tagged) - Mouse mitochondrial ribosomal protein L2 (Mrpl2), nuclear gene encoding mitochondrial protein 10 ug
$600.00
MR204304L4 Lenti ORF clone of Mrpl2 (mGFP-tagged) - Mouse mitochondrial ribosomal protein L2 (Mrpl2), nuclear gene encoding mitochondrial protein 10 ug
$600.00

Plasmid Map for MR204304

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR204304
Circular map for MR204304

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