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Cyb5r1 (NM_028057) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR204299
Cyb5r1 (Myc-DDK-tagged) - Mouse cytochrome b5 reductase 1 (Cyb5r1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cyb5r1
Synonyms 1500005G05Rik; B5R.1; C80155; Nqo3a2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR204299 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGATCCAGCCGAGCCCGGTCTTGCTAGCCTCTCTGGGGGTGGGGCTGCTTACTCTGCTCGGCCTGG
CTCTGGGTACCTACTTGGTTCGAAGATCCCGCCGGCCGCAGGTTACTCTCCAGGACCCGGATGAGAAGTA
CCTGCTGCGATTGCTAGACAAGACGACAGTGAGCCACAACACCAGGAGGTTCCGCTTTGCCTTGCCCACC
GCCCACCACATTCTGGGGCTGCCTGTGGGTAAGCACGTCTACCTCTCTGCCCGAATTGATGGCAGTCTGG
TCATCAGGCCTTACACCCCTGTCACCAGTGATGAAGACCAAGGCTATGTAGATCTTGTCATCAAGGTTTA
TCTGAAAGGTGTGCACCCCAAATTTCCTGAAGGAGGGAAGATGTCTCAATACCTGGATAGCCTGAAGATT
GGGGATATGGTGGAGTTCCGGGGGCCGAGCGGGTTGCTCAGTTATGCTGGGAAAGGGAATTTTAATATTC
AGCCCAACAAAAAATCTCCACCTGAACTGCGAGTGGCGAAGAAATTGGGAATGATTGCTGGTGGGACAGG
AATCACCCCAATGCTGCAGCTGATCCGGGCCATCTTGAAAGTCCCTGAAGATCCAACCCAGTGCTTTCTG
CTTTTTGCCAACCAGACTGAAAGGGATATAATCCTGCGGGAGGACCTAGAGGAACTTCAGGCCCAGTATC
CTAATCGCTTTAAGCTCTGGTTCACTCTGGACTCTCCTCCAGAAGACTGGACCTACAGCAAGGGCTTTGT
TACGGCTGACATGATCCAGGAGCACCTGCCTGCCCCAGCAGAGGATGTGCTGCTGCTGCTCTGTGGACCA
CCACCCATGGTGCAGCTGGCCTGCCACCCTAACTTGGATAAGCTGGGCTATTCACAAAAGATGCGATTCA
CCTAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR204299 protein sequence
Red=Cloning site Green=Tags(s)

MGIQPSPVLLASLGVGLLTLLGLALGTYLVRRSRRPQVTLQDPDEKYLLRLLDKTTVSHNTRRFRFALPT
AHHILGLPVGKHVYLSARIDGSLVIRPYTPVTSDEDQGYVDLVIKVYLKGVHPKFPEGGKMSQYLDSLKI
GDMVEFRGPSGLLSYAGKGNFNIQPNKKSPPELRVAKKLGMIAGGTGITPMLQLIRAILKVPEDPTQCFL
LFANQTERDIILREDLEELQAQYPNRFKLWFTLDSPPEDWTYSKGFVTADMIQEHLPAPAEDVLLLLCGP
PPMVQLACHPNLDKLGYSQKMRFTY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_028057
ORF Size 915 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028057.3
RefSeq Size 1626 bp
RefSeq ORF 918 bp
Locus ID 72017
UniProt ID Q9DB73
Cytogenetics 1 E4
MW 34.1 kDa
Summary NADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction.UniProtKB/Swiss-Prot Function
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"NM_028057" in other vectors (4)
SKU Description Size Price
MC207712 Cyb5r1 (untagged) - Mouse cytochrome b5 reductase 1 (Cyb5r1), (10ug) 10 ug
$330.00
MG204299 Cyb5r1 (tGFP-tagged) - Mouse cytochrome b5 reductase 1 (Cyb5r1) 10 ug
$500.00
MR204299L3 Lenti ORF clone of Cyb5r1 (Myc-DDK-tagged) - Mouse cytochrome b5 reductase 1 (Cyb5r1) 10 ug
$600.00
MR204299L4 Lenti ORF clone of Cyb5r1 (mGFP-tagged) - Mouse cytochrome b5 reductase 1 (Cyb5r1) 10 ug
$600.00

Plasmid Map for MR204299

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR204299
Circular map for MR204299

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