Timd2 (NM_134249) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR204271
Timd2 (Myc-DDK-tagged) - Mouse T-cell immunoglobulin and mucin domain containing 2 (Timd2), transcript variant 2
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Timd2
Synonyms AA816106; AU019358; AU019662; C78111; G9D3; TIM-2; Tim2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR204271 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATCAGATTCAAGTCTTCATTTCAGGCCTCATACTGCTTCTCCCAGGTGCCGTGGAGTCTCATACAG
CAGTGCAGGGGCTGGCGGGTCACCCTGTCACACTTCCATGTATTTATTCGACACACCTTGGTGGAATCGT
TCCTATGTGTTGGGGCCTAGGGGAATGCCGCCATTCTTATTGTATACGGTCACTTATCTGGACCAATGGA
TATACGGTCACACATCAGAGGAACAGTCTATACCAGCTAAAGGGGAATATTTCAGAAGGAAATGTGTCCT
TGACCATAGAGAACACTGTTGTGGGTGATGGTGGTCCCTATTGCTGTGTAGTGGAGATACCTGGAGCGTT
CCATTTTGTGGACTATATGTTGGAAGTTAAACCAGAAATTTCCACGAGTCCACCAACAAGGCCCACAGCT
ACAGGAAGACCCACAACTATTTCAACAAGATCCACACATGTACCAACATCAACCAGAGTCTCTACCTCTA
CTTCTCCAACACCAGCACACACAGAGACCTACAAACCAGAGGCCACTACATTTTATCCAGATCAGACTAC
AGCTGAGGTGACAGAAACCTTACCCGATACTCCTGCAGACTGGCATAACACTGTGACATCCTCAGATGAC
CCTTGGGATGATAACACTGAAGTAATCCCTCCACAGAAGCCACAGAAAAACCTGAATAAGGGCTTCTATG
TTGGCATCTCCATTGCAGCCCTGCTGATATTGATGCTTCTGAGCACCATGGTTATCACCAGGTACGTGGT
TATGAAAAGGAAGTCAGAATCTCTGAGCTTCGTTGCCTTCCCTATCTCTAAGATTGGAGCTTCCCCCAAA
AAAGTGGTCGAACGGACCAGATGTGAAGACCAGGTCTACATTATTGAAGACACTCCTTACCCTGAAGAAG
AGTCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR204271 protein sequence
Red=Cloning site Green=Tags(s)

MNQIQVFISGLILLLPGAVESHTAVQGLAGHPVTLPCIYSTHLGGIVPMCWGLGECRHSYCIRSLIWTNG
YTVTHQRNSLYQLKGNISEGNVSLTIENTVVGDGGPYCCVVEIPGAFHFVDYMLEVKPEISTSPPTRPTA
TGRPTTISTRSTHVPTSTRVSTSTSPTPAHTETYKPEATTFYPDQTTAEVTETLPDTPADWHNTVTSSDD
PWDDNTEVIPPQKPQKNLNKGFYVGISIAALLILMLLSTMVITRYVVMKRKSESLSFVAFPISKIGASPK
KVVERTRCEDQVYIIEDTPYPEEES

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_134249
ORF Size 915 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_134249.2
RefSeq Size 3035 bp
RefSeq ORF 918 bp
Locus ID 171284
UniProt ID Q8R183
Cytogenetics 11 B1.1
MW 33.5 kDa
Summary Probable receptor for SEMA4A involved in the regulation of T-cell function. The interaction with SEMA4A enhances T-cell activation.UniProtKB/Swiss-Prot Function
Reviews
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_134249" in other vectors (4)
SKU Description Size Price
MC201957 Timd2 (untagged) - Mouse T-cell immunoglobulin and mucin domain containing 2 (Timd2), transcript variant 2, (10ug) 10 ug
$300.00
MG204271 Timd2 (tGFP-tagged) - Mouse T-cell immunoglobulin and mucin domain containing 2 (Timd2) 10 ug
$500.00
MR204271L3 Lenti ORF clone of Timd2 (Myc-DDK-tagged) - Mouse T-cell immunoglobulin and mucin domain containing 2 (Timd2), transcript variant 2 10 ug
$600.00
MR204271L4 Lenti ORF clone of Timd2 (mGFP-tagged) - Mouse T-cell immunoglobulin and mucin domain containing 2 (Timd2), transcript variant 2 10 ug
$600.00

Plasmid Map for MR204271

Download sequence for

Cloning scheme for MR204271

Circular map for MR204271

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products