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Tef (NM_017376) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR204204
Tef (Myc-DDK-tagged) - Mouse thyrotroph embryonic factor (Tef), transcript variant 1
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Tef or NM_017376"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tef
Synonyms 2310028D20Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR204204 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCGACGCGGGCGGCGGGAAGAAGCCGCCTGTGGAGCCTCAGGCGGGGCCGGGCCCGGGGCGTGCAG
CTGGAGAACGGGGCCTGTCGGGCTCCTTCCCTCTGGTCCTGAAGAAGCTGATGGAGAACCCCCCGCGCGA
GACGCGCCTTGATAAGGAAAAGGGGAAGGAAAAGCTGGAGGAAGACGAGTCAGCAGCAGCCAGTACCATG
GCCGTCTCTGCCTCCCTCATGCCACCCATCTGGGACAAGACCATCCCCTACGATGGCGAGTCCTTCCACC
TCGAGTACATGGACCTGGATGAGTTCCTGCTGGAGAACGGCATCCCTGCCAGCCCCACCCACCTGGCACA
GAACCTGCTGCTGCCTGTGGCAGAGCTTGAAGGAAAGGAGTCTGCCAGCTCTTCCACGGCATCGCCACCA
TCTTCCTCTACTGCCATCTTTCAGCCCTCGGAAACCGTGTCCAGCACAGAATCGTCCTTGGAAAAGGAGA
GGGAGACCCCAAGTCCCATCGACCCCAGCTGTGTGGAGGTCGATGTGAACTTCAACCCTGACCCTGCCGA
CCTGGTCCTCTCTAGTGTACCAGGGGGGGAGCTCTTCAACCCTCGGAAGCACAGGTTTGCAGAGGAGGAC
CTGAAGCCCCAGCCCATGATCAAAAAAGCCAAGAAAGTCTTTGTCCCTGATGAACAGAAGGATGAAAAGT
ATTGGACAAGACGTAAGAAGAACAATGTGGCAGCTAAACGCTCCCGGGATGCCAGGCGCCTGAAGGAGAA
CCAGATCACCATCCGGGCAGCCTTCCTGGAGAAGGAGAACACAGCCCTGCGGACGGAGGTTGCCGAGCTT
CGCAAGGAGGTGGGCAAGTGCAAGACCATCGTGTCCAAGTACGAGACCAAGTACGGGCCCTTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR204204 protein sequence
Red=Cloning site Green=Tags(s)

MSDAGGGKKPPVEPQAGPGPGRAAGERGLSGSFPLVLKKLMENPPRETRLDKEKGKEKLEEDESAAASTM
AVSASLMPPIWDKTIPYDGESFHLEYMDLDEFLLENGIPASPTHLAQNLLLPVAELEGKESASSSTASPP
SSSTAIFQPSETVSSTESSLEKERETPSPIDPSCVEVDVNFNPDPADLVLSSVPGGELFNPRKHRFAEED
LKPQPMIKKAKKVFVPDEQKDEKYWTRRKKNNVAAKRSRDARRLKENQITIRAAFLEKENTALRTEVAEL
RKEVGKCKTIVSKYETKYGPL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_017376
ORF Size 903 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_017376.2, NP_059072.1
RefSeq Size 4267 bp
RefSeq ORF 906 bp
Locus ID 21685
UniProt ID Q9JLC6
Cytogenetics 15 38.25 cM
MW 33.1 kDa
Summary Transcription factor that binds to and transactivates the TSHB promoter. Binds to a minimal DNA-binding sequence 5'-TCAGAGTTATCAG-3' (By similarity). Also activates the telokin promoter in smooth muscle-specific and calcium-dependent manner.UniProtKB/Swiss-Prot Function
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"NM_017376" in other vectors (3)
SKU Description Size Price
MG204204 Tef (tGFP-tagged) - Mouse thyrotroph embryonic factor (Tef), transcript variant 1 10 ug
$500.00
MR204204L3 Lenti ORF clone of Tef (Myc-DDK-tagged) - Mouse thyrotroph embryonic factor (Tef), transcript variant 1 10 ug
$600.00
MR204204L4 Lenti ORF clone of Tef (mGFP-tagged) - Mouse thyrotroph embryonic factor (Tef), transcript variant 1 10 ug
$600.00

Plasmid Map for MR204204

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR204204
Circular map for MR204204

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