Asgr2 (NM_007493) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR204202
Asgr2 (Myc-DDK-tagged) - Mouse asialoglycoprotein receptor 2 (Asgr2)
$289.00 $450.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Asgr2
Synonyms A; AS; Asg; ASGPR2; Asgr; Asgr-2; HL-2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR204202 representing NM_007493
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGAAGGACTGTCAAGACATCCAGCAGCTGGACTCTGAGGAGAATGACCATCAGCTCAGTGGCGATG
ATGAACATGGCTCTCATGTGCAGGATCCTAGGATAGAAAATCCACACTGGAAAGGGCAGCCTCTTTCCAG
GCCCTTTCCTCAGCGCCTCTGCTCCACGTTCCGCCTCAGTCTGCTCGCCCTGGCCTTCAACATCCTCCTG
TTGGTGGTCATCTGTGTGGTTTCATCTCAAAGCATACAGCTGCAAGAAGAGTTTCGGACCCTGAAAGAAA
CCTTCAGCAACTTTTCCTCCAGCACCCTGATGGAGTTCGGGGCTCTGGACACCCTCGGAGGTAGCACAAA
CGCCATACTGACTTCCTGGTTAGCCCAACTGGAGGAGAAGCAGCAACAGCTAAAGGCAGACCATTCCACA
TTGCTCTTTCACCTGAAGCACTTCCCAATGGATCTGCGAACCCTGACCTGTCAGCTGGCGTACTTCCAGA
GCAATGGCACGGAATGCTGCCCTGTTAACTGGGTGGAGTTCGGGGGAAGCTGCTACTGGTTTTCTCGGGA
TGGGCTCACCTGGGCTGAGGCTGACCAGTACTGCCAGCTGGAGAACGCCCACCTGCTGGTCATCAACTCC
AGGGAGGAGCAGGACTTTGTTGTAAAGCACAGGAGCCAGTTTCATATTTGGATAGGTCTTACCGACAGGG
ATGGCTCCTGGAAATGGGTGGATGGAACTGATTATAGAAGCAACTACAGGAATTGGGCCTTCACTCAGCC
AGATAACTGGCAGGGCCATGAACAGGGTGGAGGTGAAGACTGTGCTGAAATCCTGTCAGATGGCCATTGG
AATGACAACTTCTGCCAGCAGGTGAACCGCTGGGTATGCGAAAAGAGACGGAACATCACCCAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR204202 representing NM_007493
Red=Cloning site Green=Tags(s)

MEKDCQDIQQLDSEENDHQLSGDDEHGSHVQDPRIENPHWKGQPLSRPFPQRLCSTFRLSLLALAFNILL
LVVICVVSSQSIQLQEEFRTLKETFSNFSSSTLMEFGALDTLGGSTNAILTSWLAQLEEKQQQLKADHST
LLFHLKHFPMDLRTLTCQLAYFQSNGTECCPVNWVEFGGSCYWFSRDGLTWAEADQYCQLENAHLLVINS
REEQDFVVKHRSQFHIWIGLTDRDGSWKWVDGTDYRSNYRNWAFTQPDNWQGHEQGGGEDCAEILSDGHW
NDNFCQQVNRWVCEKRRNITH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_007493
ORF Size 903 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007493.3, NP_031519.1
RefSeq Size 1373 bp
RefSeq ORF 906 bp
Locus ID 11890
UniProt ID P24721
Cytogenetics 11 42.99 cM
MW 35.4 kDa
Summary This gene encodes a subunit of the asialoglycoprotein receptor. This receptor is a transmembrane protein that plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins with exposed terminal galactose or N-acetylgalactosamine residues. The asialoglycoprotein receptor is a hetero-oligomeric protein composed of major and minor subunits, which are encoded by different genes. The protein encoded by this gene is the less abundant minor subunit. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene provided by RefSeq, Sep 2015
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Documents
Resources
"NM_007493" in other vectors (4)
SKU Description Size Price
MC205737 Asgr2 (untagged) - Mouse asialoglycoprotein receptor 2 (Asgr2), (10ug) 10 ug
$450.00
MG204202 Asgr2 (tGFP-tagged) - Mouse asialoglycoprotein receptor 2 (Asgr2) 10 ug
$489.00 $650.00
MR204202L3 Lenti ORF clone of Asgr2 (Myc-DDK-tagged) - Mouse asialoglycoprotein receptor 2 (Asgr2) 10 ug
$750.00
MR204202L4 Lenti ORF clone of Asgr2 (mGFP-tagged) - Mouse asialoglycoprotein receptor 2 (Asgr2) 10 ug
$750.00

Plasmid Map for MR204202

Download sequence for

Cloning scheme for MR204202

Circular map for MR204202

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