2410042D21Rik (BC012402) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR204161
2410042D21Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 2410042D21 gene (cDNA clone MGC:7119 IMAGE:3157982)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol 2410042D21Rik
Synonyms 2410042D21Rik; AA166487; AA473307; AI467442
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR204161 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCATTTGATACTCACCATGTTAAAAAACGGAACTTCTCTAATAGTATTGATCTTCCTAGAAAGAGGA
TCTCTAACTTCACCAGTAAGAACATGAAGGAGGTTAAGAGATCTCCAAAACAGTTGGCTGCTTACATAAG
TAGAACAGTTGCACAGGCAGTGAAAAGCCCAGAAAAACTGCGCAAGGTGCTCTACCACAGAAAGTTAGTT
CGTCGTTCCTTCCCAAATCCTTGTTACAAAACTAAGCAGTCCCCAAAAAGTGGGGGCTGTGACATGGCAA
ATAAAGAAAATGAACTGGCTTGTGCAGGCCATCTACCTGAAAATTTACGCCACGACAGTCGGACATTTGT
CATTAATACCAGTGATTCTGGGTCTTCACAGACAGAAAGCCCATCATCAAAATATAGTGGTTTCTTTTCT
GAAGTTTCTCAGGACCATGAGACAATGGCCCAGGTTCTATTCAGCAGGAATTTGAGATTGAATGTAGCTT
TGACTTTCTGGAGAAAGAGAAGTATAAGTGAACTTGTAGCTTATTTAGTGAGGATAGAAGACCTTGGAGT
TGTGGTAGATTGCCTTCCTGTTCTAACCAATAGTTTACAGGAAGAAAAGCAGTATATCTCACTTGGCTGC
TGTGTAGACTTACTTCCTCTGGTAAAGTCTTTACTTCAAAGCAGATTTGAAGAATATGTAATTGTTGGTT
TAAACTGGCTTCAAGCAGTAATAAAAAGGTGGTGGTCAGAACTCTCATCTACGTCAGAAATTATAAGTGA
TGGAAATATTAAGATTTTAAAGCAACAGTTGAGTGGACTATGGGAACAGGAAAGCCATCTTACTTTGGTT
CCAGGATATACTGGTAATATAGCTAAGGATGTAGATGCTTATTTATTACAGCTGCAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR204161 protein sequence
Red=Cloning site Green=Tags(s)

MAFDTHHVKKRNFSNSIDLPRKRISNFTSKNMKEVKRSPKQLAAYISRTVAQAVKSPEKLRKVLYHRKLV
RRSFPNPCYKTKQSPKSGGCDMANKENELACAGHLPENLRHDSRTFVINTSDSGSSQTESPSSKYSGFFS
EVSQDHETMAQVLFSRNLRLNVALTFWRKRSISELVAYLVRIEDLGVVVDCLPVLTNSLQEEKQYISLGC
CVDLLPLVKSLLQSRFEEYVIVGLNWLQAVIKRWWSELSSTSEIISDGNIKILKQQLSGLWEQESHLTLV
PGYTGNIAKDVDAYLLQLH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC012402
ORF Size 897 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC012402, AAH12402
RefSeq Size 1953 bp
RefSeq ORF 899 bp
Locus ID 72425
Cytogenetics 2 E3
MW 34 kDa
Summary Regulates microtubule-severing activity of KATNAL1 in a concentration-dependent manner in vitro.UniProtKB/Swiss-Prot Function
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Documents
Resources
"BC012402" in other vectors (3)
SKU Description Size Price
MG204161 2410042D21Rik (tGFP-tagged) - Mouse RIKEN cDNA 2410042D21 gene (cDNA clone MGC:7119 IMAGE:3157982) 10 ug
$500.00
MR204161L3 Lenti ORF clone of 2410042D21Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 2410042D21 gene (cDNA clone MGC:7119 IMAGE:3157982) 10 ug
$600.00
MR204161L4 Lenti ORF clone of 2410042D21Rik (mGFP-tagged) - Mouse RIKEN cDNA 2410042D21 gene (cDNA clone MGC:7119 IMAGE:3157982) 10 ug
$600.00

Plasmid Map for MR204161

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Cloning scheme for MR204161

Circular map for MR204161

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