Glod4 (NM_026029) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR204147
Glod4 (Myc-DDK-tagged) - Mouse glyoxalase domain containing 4 (Glod4)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Glod4
Synonyms 1700082G03Rik; 2700085E05Rik; C81254
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR204147 representing NM_026029
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCACTCGTCGAGCTCTGCACTTCGTTTTCAAAGTGAAGAACCGCTTCCAGACAGTGCATTTCTTTC
GAGATGTCCTGGGGATGCAGGTTCTCCGCCATGAGGAATTTGAAGAAGGCTGCAAAGCTGCATGCAATGG
GCCTTATGATGGGAAGTGGAGTAAAACAATGGTGGGATTTGGGCCCGAGGATGATCATTTTGTTGCAGAA
TTGACATACAATTATGGCATCGGAGACTACAAGCTTGGCAATGACTTCATGGGAATCACGCTTGCTTCTA
GCCAAGCTGTCAGCAATGCCAGGAAGCTGGAGTGGCCACTGAGTAAAGTTGCAGAAGGGATTTTTGAAAC
TGAAGCCCCAGGAGGATATAAGTTCTACTTACAGGATAGAAGCCCATCTCAGTCAGATCCTGTATTAAAG
GTGACTCTAGCAGTGTCTGATCTCCAGAAGTCCTTGAACTACTGGTCTAATCTCCTGGGAATGAAAATTT
ATGAGCAAGATGAGGAAAAGCAACGAGCTTTGCTGGGCTACGCTGATAACCAGTGTAAGCTAGAGCTACA
GGGCATCCAGGGTGCAGTTGATCATGCAGCGGCCTTTGGAAGAATTGCCTTTTCTTGTCCTCAGAAAGAG
TTGCCAGACTTAGAAGACTTGATGAAAAGGGAGAGCCACTCGATCCTGACTCCGTTGGTGAGTCTGGACA
CCCCAGGGAAAGCCACAGTGCAAGTGGTCATCCTGGCTGACCCTGATGGACATGAAATTTGCTTTGTTGG
GGATGAAGCTTTTCGAGAACTCTCTAAGATGGATCCAAAAGGAAGCAAATTATTGGATGATGCAATGGAA
GCAGACAAAAGTGACGAGTGGTTTGCTACGAGGAATAAACCAAAGGCTTCGGGT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR204147 representing NM_026029
Red=Cloning site Green=Tags(s)

MATRRALHFVFKVKNRFQTVHFFRDVLGMQVLRHEEFEEGCKAACNGPYDGKWSKTMVGFGPEDDHFVAE
LTYNYGIGDYKLGNDFMGITLASSQAVSNARKLEWPLSKVAEGIFETEAPGGYKFYLQDRSPSQSDPVLK
VTLAVSDLQKSLNYWSNLLGMKIYEQDEEKQRALLGYADNQCKLELQGIQGAVDHAAAFGRIAFSCPQKE
LPDLEDLMKRESHSILTPLVSLDTPGKATVQVVILADPDGHEICFVGDEAFRELSKMDPKGSKLLDDAME
ADKSDEWFATRNKPKASG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_026029
ORF Size 894 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026029.3, NP_080305.2
RefSeq Size 2523 bp
RefSeq ORF 897 bp
Locus ID 67201
UniProt ID Q9CPV4
Cytogenetics 11 B5
MW 33.3 kDa
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Documents
Resources
"NM_026029" in other vectors (4)
SKU Description Size Price
MC204909 Glod4 (untagged) - Mouse glyoxalase domain containing 4 (Glod4), (10ug) 10 ug
$300.00
MG204147 Glod4 (tGFP-tagged) - Mouse glyoxalase domain containing 4 (Glod4) 10 ug
$500.00
MR204147L3 Lenti ORF clone of Glod4 (Myc-DDK-tagged) - Mouse glyoxalase domain containing 4 (Glod4) 10 ug
$600.00
MR204147L4 Lenti ORF clone of Glod4 (mGFP-tagged) - Mouse glyoxalase domain containing 4 (Glod4) 10 ug
$600.00

Plasmid Map for MR204147

Download sequence for

Cloning scheme for MR204147

Circular map for MR204147

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