Mlx (NM_011550) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR204137
Mlx (Myc-DDK-tagged) - Mouse MAX-like protein X (Mlx), transcript variant 3
  $450.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mlx
Synonyms bHLHd13; Tcfl4; Tf4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR204137 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACGGAGCCGGGCGCCTCTCCGGAGGACCCTTGGGTCAAGGCAAGCTTCGCGGACGCGCACGCCGGCG
AGGGGAGGGCGGGTCGGGCTCGTGCACGTAGGGGGTCCGGAAGACGCGGGGCTCCCCAACTATCCCCAGA
GTCTCCCCTGCTGTCCGGGGCCCGGGGCTGCAGAGAAGACAGCTCTCACCCGGCCTGTGCCAAGGTCGAG
TATGCCTACAGTGACAACAGCCTGGATCCTGGGCTTTTTGTAGAAAGCACCCACAAGGGGAGTGTAGTGT
CCAGAGCTAATAGCATCGGCTCCACCAGTGCCTCTTCTGTCCCCAACACAGATGAGGAGGACAGTGATTA
TCAGCAGGAGTCCTACAAGGAGTCCTACAAAGACCGGAGGCGGCGTGCGCACACTCAGGCTGAACAGAAG
AGAAGGGATGCTATTAAGAGAGGCTATGATGACCTTCAGACCATTGTCCCTACCTGCCAGCAGCAGGATT
TCTCCATTGGCTCCCAAAAACTCAGCAAAGCCATCGTTCTACAGAAGACCATTGACTACATCCAGTTTTT
ACACAAGGAGAAGAAAAAGCAGGAGGAAGAAGTATCCACACTGCGCAAGGACGTCACAGCCTTGAAGATA
ATGAAAGTGAACTATGAGCAAATCGTGAAGGCACATCAGGACAACCCTCATGAGGGAGAGGACCAGGTCT
CTGACCAGGTCAAGTTCAACGTCTTTCAAGGCATCATGGACTCCCTCTTCCAGTCTTTCAACGCCTCTAT
CTCTGTGGCCAGCTTCCAGGAGCTCTCAGCTTGTGTCTTCAGCTGGATTGAGGAACACTGTGAGCCACAG
ACTCTACGAGAGATTGTGATCGGTGTCCTTCATCAGTTGAAAAACCAACTCTAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR204137 protein sequence
Red=Cloning site Green=Tags(s)

MTEPGASPEDPWVKASFADAHAGEGRAGRARARRGSGRRGAPQLSPESPLLSGARGCREDSSHPACAKVE
YAYSDNSLDPGLFVESTHKGSVVSRANSIGSTSASSVPNTDEEDSDYQQESYKESYKDRRRRAHTQAEQK
RRDAIKRGYDDLQTIVPTCQQQDFSIGSQKLSKAIVLQKTIDYIQFLHKEKKKQEEEVSTLRKDVTALKI
MKVNYEQIVKAHQDNPHEGEDQVSDQVKFNVFQGIMDSLFQSFNASISVASFQELSACVFSWIEEHCEPQ
TLREIVIGVLHQLKNQLY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_011550
ORF Size 894 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_011550.2, NP_035680.2
RefSeq Size 1992 bp
RefSeq ORF 897 bp
Locus ID 21428
UniProt ID O08609
Cytogenetics 11 D
MW 33.4 kDa
Summary Transcription regulator. Forms a sequence-specific DNA-binding protein complex with MAD1, MAD4, MNT, WBSCR14 and MLXIP which recognizes the core sequence 5'-CACGTG-3'. The TCFL4-MAD1, TCFL4-MAD4, TCFL4-WBSCR14 complexes are transcriptional repressors. Plays a role in transcriptional activation of glycolytic target genes. Involved in glucose-responsive gene regulation.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_011550" in other vectors (4)
SKU Description Size Price
MC201659 Mlx (untagged) - Mouse MAX-like protein X (Mlx), transcript variant 3, (10ug) 10 ug
$480.00
MG204137 Mlx (tGFP-tagged) - Mouse MAX-like protein X (Mlx) 10 ug
$650.00
MR204137L3 Lenti ORF clone of Mlx (Myc-DDK-tagged) - Mouse MAX-like protein X (Mlx), transcript variant 3 10 ug
$750.00
MR204137L4 Lenti ORF clone of Mlx (mGFP-tagged) - Mouse MAX-like protein X (Mlx), transcript variant 3 10 ug
$750.00

Plasmid Map for MR204137

Download sequence for

Cloning scheme for MR204137

Circular map for MR204137

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