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Mpst (NM_138670) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR204112
Mpst (Myc-DDK-tagged) - Mouse mercaptopyruvate sulfurtransferase (Mpst), nuclear gene encoding mitochondrial protein, transcript variant 1
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (5)

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Other products for "Mpst or NM_138670"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mpst
Synonyms Mst
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR204112 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGCCCCGCAACTTTTCCGAGCTCTGGTGTCTGCGCAGTGGGTGGCGGAGGCTCTGAAGGCCCCGC
GCTCCTCACAGCCGCTGAAGTTACTGGACGCGTCCTGGTACCTACCCAAGCTGGGCCGCGATGCACGACG
CGAGTTTGAGGAGCGCCACATCCCGGGCGCCGCCTTCTTCGACATCGATCGTTGCAGTGACCACACGTCC
CCCTACGACCACATGCTGCCTAACGCCACGCACTTTGCCGACTACGCGGGCAGCTTGGGCGTGAGCGCCG
CCACGCACGTCGTGATCTACGACGGCAGTGACCAGGGCCCCTATTCCGCTCCGCGGGTCTGGTGGATGTT
CCGCGCCTTCGGCCACCACTCTGTGTCATTGCTGGACGGCGGCTTCCGCCACTGGCTGAATCAGAACCTG
CCAATCAGCTCCGGCAAAAGCCACTCGGAGCCCGCGGAGTTCAGCGCGCAGCTCGATCCCTCTTTCATCA
AGACCCACGAGGACATCCTGGAGAACTTGGATGCCCGGCGCTTCCAGGTGGTGGACGCCCGCGCAGCTGG
CCGTTTCCAAGGCACCCAGCCAGAACCCCGAGATGGCATCGAACCTGGCCACATCCCTGGCTCAGTAAAC
ATCCCGTTCACTGAATTCCTGACCAACGAAGGCCTGGAGAAGAGCCCAGAGGAGATCAAACGCCTGTTTA
AGGAGAAGAAGGTGGACCTGTCTAAGCCCTTGGTAGCCACGTGTGGCTCCGGTGTCACAGCCTGCCACGT
GGTCCTGGGGGCCTTCCTCTGTGGCAAGTCAGACGTGCCTGTCTATGATGGCTCCTGGGTAGAGTGGTAC
ATGCGTGCCCAGCCAGAGCATATCATCTCTGAGGGCCGAGGGAAGACCCAG

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR204112 protein sequence
Red=Cloning site Green=Tags(s)

MAAPQLFRALVSAQWVAEALKAPRSSQPLKLLDASWYLPKLGRDARREFEERHIPGAAFFDIDRCSDHTS
PYDHMLPNATHFADYAGSLGVSAATHVVIYDGSDQGPYSAPRVWWMFRAFGHHSVSLLDGGFRHWLNQNL
PISSGKSHSEPAEFSAQLDPSFIKTHEDILENLDARRFQVVDARAAGRFQGTQPEPRDGIEPGHIPGSVN
IPFTEFLTNEGLEKSPEEIKRLFKEKKVDLSKPLVATCGSGVTACHVVLGAFLCGKSDVPVYDGSWVEWY
MRAQPEHIISEGRGKTQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-RsrII Cloning Scheme for this gene |
ACCN NM_138670
ORF Size 891 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_138670.1
RefSeq Size 1426 bp
RefSeq ORF 894 bp
Locus ID 246221
UniProt ID Q99J99
Cytogenetics 15 E1
MW 33 kDa
Summary Transfer of a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Detoxifies cyanide and is required for thiosulfate biosynthesis. Acts as an antioxidant. In combination with cysteine aminotransferase (CAT), contributes to the catabolism of cysteine and is an important producer of hydrogen sulfide in the brain, retina and vascular endothelial cells. Hydrogen sulfide H(2)S is an important synaptic modulator, signaling molecule, smooth muscle contractor and neuroprotectant. Its production by the 3MST/CAT pathway is regulated by calcium ions.UniProtKB/Swiss-Prot Function
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"NM_138670" in other vectors (4)
SKU Description Size Price
MC207936 Mpst (untagged) - Mouse mercaptopyruvate sulfurtransferase (Mpst), nuclear gene encoding mitochondrial protein, transcript variant 1, (10ug) 10 ug
$330.00
MG204112 Mpst (tGFP-tagged) - Mouse mercaptopyruvate sulfurtransferase (Mpst) 10 ug
$500.00
MR204112L3 Lenti ORF clone of Mpst (Myc-DDK-tagged) - Mouse mercaptopyruvate sulfurtransferase (Mpst), nuclear gene encoding mitochondrial protein, transcript variant 1 10 ug
$600.00
MR204112L4 Lenti ORF clone of Mpst (mGFP-tagged) - Mouse mercaptopyruvate sulfurtransferase (Mpst), nuclear gene encoding mitochondrial protein, transcript variant 1 10 ug
$600.00

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