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Myoz1 (NM_021508) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR204094
Myoz1 (Myc-DDK-tagged) - Mouse myozenin 1 (Myoz1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Myoz1
Synonyms 2310001N11Rik; AV090278; FATZ; Myoz
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR204094 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCACTCTCAGGAACCCCGGCCCCTAACAAGAGGAGGAAGTCAAGCAAACTGATTATGGAGCTCACTG
GAGGTGGCCGGGAGAGCTCAGGCCTGAACCTGGGCAAGAAGATCAGTGTCCCAAGGGATGTGATGTTGGA
GGAGCTGTCCCTTCTTACCAACCGAGGCTCCAAGATGTTCAAGCTACGGCAGATGCGGGTGGAGAAATTT
ATCTATGAGAATCACCCCGATGTTTTCTCTGACAGCTCAATGGATCACTTCCAGAAGTTTCTTCCCACAG
TGGGAGGACAGCTGGAGACAGCTGGTCAGGGCTTCTCATATGGCAAGGGCAGCAGTGGAGGCCAGGCTGG
CAGCAGTGGCTCTGCTGGACAGTATGGCTCTGACCGTCATCAGCAGGGCTCTGGGTTTGGAGCTGGGGGT
TCAGGTGGTCCTGGGGGCCAGGCTGGTGGAGGAGGAGCTCCTGGCACAGTAGGGCTTGGAGAGCCCGGAT
CAGGTGACCAGGCAGGTGGAGATGGAAAACATGTCACTGTGTTCAAGACTTATATTTCCCCATGGGATCG
GGCCATGGGGGTTGATCCTCAGCAAAAAGTGGAACTTGGCATTGACCTACTGGCATACGGTGCCAAAGCT
GAACTCCCCAAATATAAGTCCTTCAACAGGACAGCAATGCCCTACGGTGGATATGAGAAGGCCTCCAAAC
GCATGACCTTCCAGATGCCCAAGTTTGACCTGGGGCCTCTGCTGAGTGAACCCCTGGTCCTCTACAACCA
GAACCTCTCCAACAGGCCTTCTTTCAATCGAACCCCTATTCCCTGGTTGAGCTCTGGGGAGCATGTAGAC
TACAACGTGGATGTTGGTATCCCCTTGGATGGAGAGACAGAGGAGCTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR204094 protein sequence
Red=Cloning site Green=Tags(s)

MPLSGTPAPNKRRKSSKLIMELTGGGRESSGLNLGKKISVPRDVMLEELSLLTNRGSKMFKLRQMRVEKF
IYENHPDVFSDSSMDHFQKFLPTVGGQLETAGQGFSYGKGSSGGQAGSSGSAGQYGSDRHQQGSGFGAGG
SGGPGGQAGGGGAPGTVGLGEPGSGDQAGGDGKHVTVFKTYISPWDRAMGVDPQQKVELGIDLLAYGAKA
ELPKYKSFNRTAMPYGGYEKASKRMTFQMPKFDLGPLLSEPLVLYNQNLSNRPSFNRTPIPWLSSGEHVD
YNVDVGIPLDGETEEL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_021508
ORF Size 888 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_021508.3, NP_067483.1
RefSeq Size 1294 bp
RefSeq ORF 891 bp
Locus ID 59011
UniProt ID Q9JK37
Cytogenetics 14 A3
MW 31.5 kDa
Summary Myozenins may serve as intracellular binding proteins involved in linking Z-disk proteins such as alpha-actinin, gamma-filamin, TCAP/telethonin, LDB3/ZASP and localizing calcineurin signaling to the sarcomere. Plays an important role in the modulation of calcineurin signaling. May play a role in myofibrillogenesis.UniProtKB/Swiss-Prot Function
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"NM_021508" in other vectors (4)
SKU Description Size Price
MC204093 Myoz1 (untagged) - Mouse myozenin 1 (Myoz1), (10ug) 10 ug
$300.00
MG204094 Myoz1 (tGFP-tagged) - Mouse myozenin 1 (Myoz1) 10 ug
$500.00
MR204094L3 Lenti ORF clone of Myoz1 (Myc-DDK-tagged) - Mouse myozenin 1 (Myoz1) 10 ug
$600.00
MR204094L4 Lenti ORF clone of Myoz1 (mGFP-tagged) - Mouse myozenin 1 (Myoz1) 10 ug
$600.00

Plasmid Map for MR204094

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR204094
Circular map for MR204094

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