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Dppa4 (NM_028610) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR204087
Dppa4 (Myc-DDK-tagged) - Mouse developmental pluripotency associated 4 (Dppa4), transcript variant 1
Interest in protein/lysate? Submit request here!
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Dppa4
Synonyms 2410091M23Rik; C76608; ECAT15-1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR204087 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGACTGCTGGAGACAAGAAGTGGAGCGCAGAAGAGCCGAAGGAAGAAGTGGAATTGCAGATGTCTA
GTCAACCAGGCACGGCTCCTGCAAAGGCTAAAGCAACGGGGAAAAAACAAAAGAAGTCGGAGACAGATAA
TGGTTGTAAACCAAAGGAGGGAAGACCACAAGACACTGAGACGCCAGGACAGACTCGTAGGAAGGTACCA
ATTCCTCCTATTCCAGAGTATCTGCCCCCAGTGAACCTGATTCACCGAGATGTTTTGCGGGCATGGTGCC
AGAAGAAACGAGTGAGCAGCAAAGGCCAGAAATTAGATGCTTATAAACGACTCCTTGCAAGGGCTTTCCC
AGAACAAATGCTGGAGTTGAGGAACGTCCCTGACTCAGCCAAAGACGCCAGGTTGAAGACAGCTCACAAA
AAAATGAAGACTGAACCGGGGGAGGAGTCTGAGGTGACAGTTCCTCTGGAAATGGTCCCTGTGCCAGAGG
AGCAGATACCTGCCCTCATTGACCCTCCTATGCTCTATGAGGAAGTCAGCACCACCGTAGTGACTACACC
TGCCACTGAGGCCGTGTTAGCATCTTGGGCCAGAATTGCATCCAATGCTAAGAAGTACGAGGCAGTGCCA
GCCGATGCTTCGTCCTCATCAGAAGTCAAAGGGGAAATGTGGTGTGTGGTTCATGGGACCAGCCTTCCTG
GGAACTCGCGTGGTTGGGTTCGGCTGCAGTTCCACGCTGGACAAGCCTGGGTACCCGATAAGAAAGGAAA
AGCCATTGCCCTCTTCCTGCTTCCGGCCTGCACATTTCCACCCCCACACCTGGAGGACAACATGCTGTGC
CCCAAGTGTGTTCATAAGAACAAGATCTTGACTAAGAGCCTCGAAGGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR204087 protein sequence
Red=Cloning site Green=Tags(s)

METAGDKKWSAEEPKEEVELQMSSQPGTAPAKAKATGKKQKKSETDNGCKPKEGRPQDTETPGQTRRKVP
IPPIPEYLPPVNLIHRDVLRAWCQKKRVSSKGQKLDAYKRLLARAFPEQMLELRNVPDSAKDARLKTAHK
KMKTEPGEESEVTVPLEMVPVPEEQIPALIDPPMLYEEVSTTVVTTPATEAVLASWARIASNAKKYEAVP
ADASSSSEVKGEMWCVVHGTSLPGNSRGWVRLQFHAGQAWVPDKKGKAIALFLLPACTFPPPHLEDNMLC
PKCVHKNKILTKSLEG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_028610
ORF Size 888 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028610.2, NP_082886.2
RefSeq Size 1534 bp
RefSeq ORF 891 bp
Locus ID 73693
UniProt ID Q8CCG4
Cytogenetics 16 B5
MW 32.7 kDa
Summary May be involved in the maintenance of active epigenetic status of target genes. May inhibit differentiation of embryonic stem (ES) cells into a primitive ectoderm lineage.UniProtKB/Swiss-Prot Function
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Resources
"NM_028610" in other vectors (2)
SKU Description Size Price
MR204087L3 Lenti ORF clone of Dppa4 (Myc-DDK-tagged) - Mouse developmental pluripotency associated 4 (Dppa4), transcript variant 1 10 ug
$600.00
MR204087L4 Lenti ORF clone of Dppa4 (mGFP-tagged) - Mouse developmental pluripotency associated 4 (Dppa4), transcript variant 1 10 ug
$600.00

Plasmid Map for MR204087

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file-download TXT (FASTA format)

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Cloning scheme for MR204087
Circular map for MR204087

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