Cln8 (NM_012000) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR203926
Cln8 (Myc-DDK-tagged) - Mouse ceroid-lipofuscinosis, neuronal 8 (Cln8)
  $450.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cln8
Synonyms mnd
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR203926 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACTCCTGTGAGCAGCCACGGCTTGGCGGAGAGCATTTTTGACCTGGACTACGCTTCGTGGAAGATTC
GGTCGACTCTAGCGGTTGCTGGCTTTGTCTTCTACCTGGGCGTCTTTGTAGTCTGCCATCAGCTCTCATC
GTCCCTGAATGCCACCTACCGCTCCCTGGCGGCCAAAGAGAAGGTCTTCTGGAACCTGGCGGCGACGCGT
GCTGTCTTCGGCGTCCAGAGCACAACTGCAGGCCTGTGGGCCCTGCTGGGAGACCCCGTGCTCTATGCCG
ACAAAGCTCTCGGGCAGCAGAACTGGTGTTGGTTTCACATCACCACGGCCACTGGCTTCTTCTTCTTTGA
GAATGTGGCCGTTCACCTGTCCAACCTGTTCTTCCGCACCTTTGACTTGTTTCTGGTTGTCCACCACCTC
TTTGCCTTTCTTGGGTTCCTGGGTTCAGCGATCAATCTCAGAGCTGGCCACTATCTAGCCATGACCACGT
TGCTCCTAGAGATGAGCACGCCCTTCACCTGCATTTCCTGGATGCTCCTGAAGGCTGGGTGGTCAGACTC
CCTGTTCTGGAAGGCCAACCAGTGGCTAATGATCCACATGTTTCACTGTCGAATGATCCTCACCTACCAC
ATGTGGTGGGTGTGCTTCTGTCACTGGGACGCCCTCACCAGCAGCCTGCACCTGCCCCACTGGGCACTGT
TCCTTTTCGGGCTGGCCCTGCTCACGGCCGTCATTAACCCTTACTGGACACACAAGAAGACCCAGCAGCT
CCTCCATCCCGTGGATTGGAACTTTGCACAGGAGGAAGCCAAGGGCAGCAGGCAAGAAAGGACCAATGGC
CAGGTGCCTCGGAAAAAGAGGTTG


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR203926 protein sequence
Red=Cloning site Green=Tags(s)

MTPVSSHGLAESIFDLDYASWKIRSTLAVAGFVFYLGVFVVCHQLSSSLNATYRSLAAKEKVFWNLAATR
AVFGVQSTTAGLWALLGDPVLYADKALGQQNWCWFHITTATGFFFFENVAVHLSNLFFRTFDLFLVVHHL
FAFLGFLGSAINLRAGHYLAMTTLLLEMSTPFTCISWMLLKAGWSDSLFWKANQWLMIHMFHCRMILTYH
MWWVCFCHWDALTSSLHLPHWALFLFGLALLTAVINPYWTHKKTQQLLHPVDWNFAQEEAKGSRQERTNG
QVPRKKRL

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_012000
ORF Size 864 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_012000.3, NP_036130.1
RefSeq Size 6432 bp
RefSeq ORF 867 bp
Locus ID 26889
UniProt ID Q9QUK3
Cytogenetics 8 7.59 cM
MW 33.1 kDa
Summary Could play a role in cell proliferation during neuronal differentiation and in protection against cell death.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_012000" in other vectors (4)
SKU Description Size Price
MC200906 Cln8 (untagged) - Mouse ceroid-lipofuscinosis, neuronal 8 (Cln8), (10ug) 10 ug
$450.00
MG203926 Cln8 (tGFP-tagged) - Mouse ceroid-lipofuscinosis, neuronal 8 (Cln8) 10 ug
$650.00
MR203926L3 Lenti ORF clone of Cln8 (Myc-DDK-tagged) - Mouse ceroid-lipofuscinosis, neuronal 8 (Cln8) 10 ug
$750.00
MR203926L4 Lenti ORF clone of Cln8 (mGFP-tagged) - Mouse ceroid-lipofuscinosis, neuronal 8 (Cln8) 10 ug
$750.00

Plasmid Map for MR203926

Download sequence for

Cloning scheme for MR203926

Circular map for MR203926

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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