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Tbc1d22a (BC023106) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR203922
Tbc1d22a (Myc-DDK-tagged) - Mouse TBC1 domain family, member 22a (cDNA clone MGC:28337 IMAGE:4016550)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tbc1d22a
Synonyms MGC28337
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR203922 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACATGGAAACTTCTCTCAGGTTACCTTCCTGCCAATGTTGACCGAAGACCAGCCACGCTGCAGAGGA
AACAGAAGGAATACTTTGCTTTCATTGAGCATTATTACAGCTCTAGGAATGACGAAGTTCACCAGGACAC
GTATCGCCAGATCCATATCGACATCCCTCGGATGAGCCCGGAAGCACTGATTCTTCAGCCCAAGGTGACA
GAGATTTTCGAAAGGATCTTGTTTATATGGGCAATCCGACATCCTGCCAGTGGATACGTTCAGGGAATAA
ATGACCTGGTCACTCCGTTCTTCGTGGTCTTCATTTGCGAGTACACAGACCGGGAGGACGTGGACAAGGT
CGATGTCTCCAGTGTGCCTGCAGAGGTGCTTCGCAACATTGAGGCTGACACCTACTGGTGCATGAGCAAG
CTTCTGGATGGCATTCAGGACAACTACACCTTTGCCCAACCTGGGATTCAGATGAAAGTGAAGATGCTGG
AAGAGCTTGTGAGCCGGATTGATGAGCGAGTCCACCGGCATCTGGACGGGCACGAGGTGCGGTACCTGCA
GTTTGCCTTCCGCTGGATGAACAACCTGCTGATGAGGGAGCTGCCCCTGCGCTGCACCATCCGCCTGTGG
GACACTTACCAGTCTGAACCCGAGGGCTTTTCTCATTTCCACTTATATGTTTGTGCTGCTTTCCTCGTGA
GATGGAGGAGAGAAATTCTAGAAGAAAGAGATTTTCAAGAGTTGCTGCTCTTCCTCCAGAACCTGCCTAC
TGCCCGCTGGGATGACCAGGATGTCAGCCTACTGCTGGCTGAGGCCTATCGCCTCAAGTTTGCCTTCGCA
GACGCCCCCAATCACTACAAGAAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR203922 protein sequence
Red=Cloning site Green=Tags(s)

MTWKLLSGYLPANVDRRPATLQRKQKEYFAFIEHYYSSRNDEVHQDTYRQIHIDIPRMSPEALILQPKVT
EIFERILFIWAIRHPASGYVQGINDLVTPFFVVFICEYTDREDVDKVDVSSVPAEVLRNIEADTYWCMSK
LLDGIQDNYTFAQPGIQMKVKMLEELVSRIDERVHRHLDGHEVRYLQFAFRWMNNLLMRELPLRCTIRLW
DTYQSEPEGFSHFHLYVCAAFLVRWRREILEERDFQELLLFLQNLPTARWDDQDVSLLLAEAYRLKFAFA
DAPNHYKK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC023106
ORF Size 864 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC023106, AAH23106
RefSeq Size 2651 bp
RefSeq ORF 866 bp
Locus ID 223754
Cytogenetics 15 40.56 cM
MW 34.5 kDa
Summary May act as a GTPase-activating protein for Rab family protein(s).UniProtKB/Swiss-Prot Function
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"BC023106" in other vectors (4)
SKU Description Size Price
MC217994 Tbc1d22a (untagged) - Mouse TBC1 domain family, member 22a (cDNA clone MGC:28337 IMAGE:4016550), (10ug) 10 ug
$330.00
MG203922 Tbc1d22a (tGFP-tagged) - Mouse TBC1 domain family, member 22a (cDNA clone MGC:28337 IMAGE:4016550) 10 ug
$500.00
MR203922L3 Lenti ORF clone of Tbc1d22a (Myc-DDK-tagged) - Mouse TBC1 domain family, member 22a (cDNA clone MGC:28337 IMAGE:4016550) 10 ug
$600.00
MR203922L4 Lenti ORF clone of Tbc1d22a (mGFP-tagged) - Mouse TBC1 domain family, member 22a (cDNA clone MGC:28337 IMAGE:4016550) 10 ug
$600.00

Plasmid Map for MR203922

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR203922
Circular map for MR203922

file-alt Citations

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