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Tmem163 (NM_028135) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR203920
Tmem163 (Myc-DDK-tagged) - Mouse transmembrane protein 163 (Tmem163)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tmem163
Synonyms 2610024A01Rik; SV31
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR203920 representing NM_028135
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCCGGCTCTGGGCAGCGAGCGCCGCAGCCCCCCTGGCCCCGGAGTCCCGCGGCCGCCACCGCGGG
GCCACGCGCCCTCGACCGCCGCCCCCGCCCCCAGCCCGGCTCCGATGAGCTCCTCTGTGCAGTCTGATGA
GGAAAGGCAGCCACGGATCAGCGAGAGCGGCCAGTTCAGCGACGGGTTGGAAGACCGAGGTTTACTAGAA
AGCAGCACCCGGCTGAAACCTCATGAAGCCCAGAACTACAGGAAGAAGGCATTGTGGGTATCCTGGCTGT
CCATTATTGTCACCCTGGCCCTGGCAGTGGCCGCCTTCACTGTCTCCGTCATGAGGTACAGCGCCTCTGC
CTTCGGGTTTGCATTTGATGCCATCCTGGATGTGCTGTCCTCGGCCATTGTCCTCTGGCGTTACAGCAAT
GCGGCTGCTGTGCACTCTGCAAACAGGGAGTATATAGCCTGTGTCATCTTGGGGGTGATCTTTCTTCTGT
CATCTATATGCATAGTGGTCAAAGCCATCCATGACCTCTCAACTAGGCTGCTCCCTGAAGTGGATGATTT
CCTGTTCAGCGTCTCCATTCTAAGCGGGATCCTTTGCAGCGTACTGGCTGTGCTGAAGTTTATGCTGGGG
AAGGTCCTGACAAGCAGGGCTCTCATCACAGACGGGTTCAACTCCCTTGTTGGGGGCGTGATGGGCTTCT
CCATCCTTCTAAGTGCAGAAGTGTTCAAACACAACGCAGCAGTCTGGTACCTGGACGGCAGCATTGGTGT
CCTGATTGGTCTCACCATCTTTGCCTACGGGGTCAAACTCCTCATCGACATGGTGCCCAGAGTGAGACAG
ACGCGTCATTATGAAATGTTTGAG

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR203920 representing NM_028135
Red=Cloning site Green=Tags(s)

MEPALGSERRSPPGPGVPRPPPRGHAPSTAAPAPSPAPMSSSVQSDEERQPRISESGQFSDGLEDRGLLE
SSTRLKPHEAQNYRKKALWVSWLSIIVTLALAVAAFTVSVMRYSASAFGFAFDAILDVLSSAIVLWRYSN
AAAVHSANREYIACVILGVIFLLSSICIVVKAIHDLSTRLLPEVDDFLFSVSILSGILCSVLAVLKFMLG
KVLTSRALITDGFNSLVGGVMGFSILLSAEVFKHNAAVWYLDGSIGVLIGLTIFAYGVKLLIDMVPRVRQ
TRHYEMFE

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_028135
ORF Size 864 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028135.2, NP_082411.1
RefSeq Size 2517 bp
RefSeq ORF 867 bp
Locus ID 72160
UniProt ID Q8C996
Cytogenetics 1 E3
MW 31.6 kDa
Summary May bind zinc and other divalent cations and recruit them to vesicular organelles.UniProtKB/Swiss-Prot Function
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Documents
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"NM_028135" in other vectors (4)
SKU Description Size Price
MC211298 Tmem163 (untagged) - Mouse transmembrane protein 163 (Tmem163), (10ug) 10 ug
$330.00
MG203920 Tmem163 (tGFP-tagged) - Mouse transmembrane protein 163 (Tmem163) 10 ug
$500.00
MR203920L3 Lenti ORF clone of Tmem163 (Myc-DDK-tagged) - Mouse transmembrane protein 163 (Tmem163) 10 ug
$600.00
MR203920L4 Lenti ORF clone of Tmem163 (mGFP-tagged) - Mouse transmembrane protein 163 (Tmem163) 10 ug
$600.00

Plasmid Map for MR203920

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR203920
Circular map for MR203920

file-alt Citations

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