Pqlc2 (NM_145384) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR203868
Pqlc2 (Myc-DDK-tagged) - Mouse PQ loop repeat containing 2 (Pqlc2)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pqlc2
Synonyms BC019216
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR203868 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACCAGGCCCTATCACTGTGGTTCCTGCTGGGCTGGATCGGTGGAGATTCCTGCAACCTCATTGGCT
CCTTTCTGGCAGACCAGCTACCCCTGCAGACCTACACGGCTGTGTATTATGTCCTGGCCGACCTGATGAT
GCTGACGCTGTACTTCCATTATAAGTTCAAGAAGCGACCTTCTCCGTTGTCTGCCCCTATCAATTCTGTG
CTCTTGTTCATCTTGGGGACGGTGTGTATCACACCACTGTTGAGCAGCACTGATCCTGTGGCTGTCCCCA
GGGAAGGCTTCCGGGGGCGGACACTCCTGTCTGTGGAGCCAGGCAATAAGCCCTTCACAAAGAAGGAGGT
CATCGGCTTTGTCATCGGCTCCGCCTCCAGCTTGCTGTACCTGCTCTCGAGGCTGCCTCAGATTCGCACC
AATTTCATACGGCAGTCAACCCAAGGCATCTCCTACTCGCTGTTCGCACTGGTGATGCTGGGGAACACGC
TGTACGGGCTCAGCGTGCTGCTCAAGAACCCCGAGGTGGGCCAGAGTGAGGGCAGCTACCTGCTGCACCA
CCTGCCCTGGCTCGTGGGCAGCCTGGGGGTGCTGCTGCTCGACACCATCGTATCCTTCAAAGCGTGTGAT
GTTGGCACCGTTGTGTGGGCAAGACGGCCTCTGCTTCAGTGTGCACATCCCTGCCAGCCCACCCCTCTGT
CTACATACTCATCCACCTGCCCATTAGCACATTCACCTATTCGTCCGTGTGTCCTTCCATCTACAATCCA
GCCTTTAACCACCCTTCATTTACCCATCCAACCTCATCCACCCACCCATCACCCACTTAGACACCTATCC
ATCTATGCACTCACC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR203868 protein sequence
Red=Cloning site Green=Tags(s)

MDQALSLWFLLGWIGGDSCNLIGSFLADQLPLQTYTAVYYVLADLMMLTLYFHYKFKKRPSPLSAPINSV
LLFILGTVCITPLLSSTDPVAVPREGFRGRTLLSVEPGNKPFTKKEVIGFVIGSASSLLYLLSRLPQIRT
NFIRQSTQGISYSLFALVMLGNTLYGLSVLLKNPEVGQSEGSYLLHHLPWLVGSLGVLLLDTIVSFKACD
VGTVVWARRPLLQCAHPCQPTPLSTYSSTCPLAHSPIRPCVLPSTIQPLTTLHLPIQPHPPTHHPLRHLS
IYALT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_145384
ORF Size 855 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_145384.1, NP_663359.1
RefSeq Size 2047 bp
RefSeq ORF 882 bp
Locus ID 212555
UniProt ID Q8C4N4
Cytogenetics 4 D3
MW 31.4 kDa
Summary Amino acid transporter that specifically mediates the pH-dependent export of the cationic amino acids arginine, histidine and lysine from lysosomes.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_145384" in other vectors (4)
SKU Description Size Price
MC202975 Pqlc2 (untagged) - Mouse PQ loop repeat containing 2 (Pqlc2), (10ug) 10 ug
$300.00
MG203868 Pqlc2 (tGFP-tagged) - Mouse PQ loop repeat containing 2 (Pqlc2) 10 ug
$500.00
MR203868L3 Lenti ORF clone of Pqlc2 (Myc-DDK-tagged) - Mouse PQ loop repeat containing 2 (Pqlc2) 10 ug
$600.00
MR203868L4 Lenti ORF clone of Pqlc2 (mGFP-tagged) - Mouse PQ loop repeat containing 2 (Pqlc2) 10 ug
$600.00

Plasmid Map for MR203868

Download sequence for

Cloning scheme for MR203868

Circular map for MR203868

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