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Vsig4 (NM_177789) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR203780
Vsig4 (Myc-DDK-tagged) - Mouse V-set and immunoglobulin domain containing 4 (Vsig4)
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Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Vsig4
Synonyms A530061A11; BC025105; CRIg; Z39IG
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR203780 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGATCTCATCAGGCTTGCTGTTCCTGGGCCACCTAATAGTGCTCACCTATGGCCACCCCACCCTAA
AAACACCTGAGAGTGTGACAGGGACCTGGAAAGGAGATGTGAAGATTCAGTGCATCTATGATCCCCTGAG
AGGCTACAGGCAAGTTTTGGTGAAATGGCTGGTAAGACACGGCTCTGACTCCGTCACCATCTTCCTACGT
GACTCCACTGGAGACCATATCCAGCAGGCAAAGTACAGAGGCCGCCTGAAAGTGAGCCACAAAGTTCCAG
GAGATGTGTCCCTCCAAATAAATACCCTGCAGATGGATGACAGGAATCACTATACATGTGAGGTCACCTG
GCAGACTCCTGATGGAAACCAAGTAATAAGAGATAAGATCATTGAGCTCCGTGTTCGGAAATATAATCCA
CCTAGAATCAATACTGAAGCACCTACAACCCTGCACTCCTCTTTGGAAGCAACAACTATAATGAGTTCAA
CCTCTGACTTGACCACTAATGGGACTGGAAAACTTGAGGAGACCATTGCTGGTTCAGGGAGGAACCTGCC
AATCTTTGCCATAATCTTCATCATCTCCCTTTGCTGCATAGTAGCTGTCACCATACCTTATATCTTGTTC
CGCTGCAGGACATTCCAACAAGAGTATGTCTATGGAGTGAGCAGGGTGTTTGCCAGGAAGACAAGCAACT
CTGAAGAAACCACAAGGGTGACTACCATCGCAACTGATGAACCAGATTCCCAGGCTCTGATTAGTGACTA
CTCTGATGATCCTTGCCTCAGCCAGGAGTACCAAATAACCATCAGATCAACAATGTCTATTCCTGCCTGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR203780 protein sequence
Red=Cloning site Green=Tags(s)

MEISSGLLFLGHLIVLTYGHPTLKTPESVTGTWKGDVKIQCIYDPLRGYRQVLVKWLVRHGSDSVTIFLR
DSTGDHIQQAKYRGRLKVSHKVPGDVSLQINTLQMDDRNHYTCEVTWQTPDGNQVIRDKIIELRVRKYNP
PRINTEAPTTLHSSLEATTIMSSTSDLTTNGTGKLEETIAGSGRNLPIFAIIFIISLCCIVAVTIPYILF
RCRTFQQEYVYGVSRVFARKTSNSEETTRVTTIATDEPDSQALISDYSDDPCLSQEYQITIRSTMSIPAC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_177789
ORF Size 840 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_177789.5
RefSeq Size 1432 bp
RefSeq ORF 843 bp
Locus ID 278180
Cytogenetics X C3
MW 31.5 kDa
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"NM_177789" in other vectors (3)
SKU Description Size Price
MC207949 Vsig4 (untagged) - Mouse V-set and immunoglobulin domain containing 4 (Vsig4), (10ug) 10 ug
$330.00
MR203780L3 Lenti ORF clone of Vsig4 (Myc-DDK-tagged) - Mouse V-set and immunoglobulin domain containing 4 (Vsig4) 10 ug
$600.00
MR203780L4 Lenti ORF clone of Vsig4 (mGFP-tagged) - Mouse V-set and immunoglobulin domain containing 4 (Vsig4) 10 ug
$600.00

Plasmid Map for MR203780

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file-download TXT (FASTA format)

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Cloning scheme for MR203780
Circular map for MR203780

file-alt Citations

VSIG4 inhibits proinflammatory macrophage activation by reprogramming mitochondrial pyruvate metabolism
Li, Diao, Guo et al
Nat Commun (2017) 8 (1), 1322 DOI: 10.1038/s41467-017-01327-4
AI Summary: OriGene products, specifically the mouse Vsig4 cDNA ORF clone (#MR203780) and Pdk2 cDNA ORF clone (#MG206400), were used in this study to enable gene expression and functional analyses of VSIG4 and PDK2 in macrophage immunometabolism.
Snippet: The mouseVsig4 (NM_177789) cDNA ORF clone (#MR203780) and Pdk2 (NM_133667) cDNA ORF clone (#MG206400) were purchased from OriGene Technologies, Inc. (Rockville, MD, USA). The whole gene expression cDNA for Vsig4, Pdk2, the truncation mutants of Vsig4, and Vsig4 site-directed mutagenesis were further amplified with specific primers (Supplementary Table 3)
FigureFigureFigure
Showing 1-1 of 1 papers.
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