Casp3 (NM_009810) Mouse Tagged ORF Clone
$450.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Casp3 |
| Synonyms | A830040C14Rik; AC-; AC-3; Casp; CASP-3; Caspase-3; CC3; CPP; CPP-32; CPP32; Lice; mld; mldy; SCA-1; Ya; Yama |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_009810 |
| ORF Size | 831 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_009810.3 |
| RefSeq Size | 1466 bp |
| RefSeq ORF | 834 bp |
| Locus ID | 12367 |
| UniProt ID | P70677 |
| Cytogenetics | 8 26.39 cM |
| MW | 31.9 kDa |
| Summary | This gene encodes a protein that belongs to a highly conserved family of cysteinyl aspartate-specific proteases that function as essential regulators of programmed cell death through apoptosis. Members of this family contain an N-terminal pro-domain and require cleavage at specific aspartate residues to become mature. The protein encoded by this gene belongs to a subgroup of cysteinyl aspartate-specific proteases that are activated by initiator caspases and that perform the proteolytic cleavage of apoptotic target proteins. Mice defective for this gene exhibit a variety of phenotypes including reduced neuronal apoptosis resulting in hyperplasias, hearing loss, attenuated osteogenic differentiation of bone marrow stromal stem cells, and pre- and post-natal lethality. Alternative splicing results in multiple transcript variants. provided by RefSeq, Sep 2015 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_009810" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC208217 | Casp3 (untagged) - Mouse caspase 3 (Casp3), (10ug) | 10 ug |
$450.00
|
|
| MG203736 | Casp3 (tGFP-tagged) - Mouse caspase 3 (Casp3) | 10 ug |
$650.00
|
|
| MR203736L3 | Lenti ORF clone of Casp3 (Myc-DDK-tagged) - Mouse caspase 3 (Casp3) | 10 ug |
$750.00
|
|
| MR203736L4 | Lenti ORF clone of Casp3 (mGFP-tagged) - Mouse caspase 3 (Casp3) | 10 ug |
$750.00
|
|
Citations
Cutting Edge: Mitochondrial Assembly of the NLRP3 Inflammasome Complex Is Initiated at Priming
Elliott, Miller, Banoth et al
J. Immunol. (2018) 200 (9), 3047-3052 DOI: 10.4049/jimmunol.1701723
Elliott, Miller, Banoth et al
J. Immunol. (2018) 200 (9), 3047-3052 DOI: 10.4049/jimmunol.1701723
AI Summary: OriGene Technologies provided pCMV6 entry plasmids expressing murine caspase-1 and caspase-3 with C-terminal Myc and DDK (FLAG) tags, as well as empty and His-tagged vector controls, which were essential for protein expression and functional studies in this paper.
Snippet: Plasmids expressing murine caspase-1 and caspase-3 with C-terminal Myc and DDK (FLAG) tags (pCMV6 entry) and empty vectors for expression of N-terminal Myc-DDK (pCMV6-AN-Myc-DDK) and N- and C-terminal His-tagged proteins (pCMV6-AN-His and pCMV6-AC-His, respectively) were obtained from OriGene Technologies. …
Showing 1-1 of 1 papers.
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